Identification and determination of tanshinone ⅡA in Compound Granule Tetrandra
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摘要: 目的 建立复方汉防己颗粒中丹参酮ⅡA的成分鉴别和含量测定方法。 方法 采用薄层色谱法对丹参酮ⅡA进行定性鉴别;采用高效液相色谱法对其进行含量测定。 结果 薄层鉴别斑点清晰,阴性对照无干扰;含量测定结果表明丹参酮ⅡA进样量在5.28~52.8 μg/ml范围内线性关系良好(r=0.999 9),平均加样回收率为97.97%,RSD为0.34%(n=9)。 结论 本法操作简便、专属性强、重现性好,可用于复方汉防己颗粒中丹参酮ⅡA的质量控制。Abstract: Objective To develop a method for identification and determination of tanshinone ⅡA in Compound Granule Tetrandra. Methods TLC method was used to identify tanshinone ⅡA, HPLC method was used to determine the content. Results The spot was clear in TLC identification without interference of negative control.The sample size of tanshinone ⅡA had a good linear relationship with peak area(r=0.999 9), when ranged from 5.28~52.8 μg/ml, the average recovery rate was 97.97%, RSD was 0.34%(n=9). Conclusion The method was simple, with good specificity and reproducibility, which could be used as the quality control method for this preparation.
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Key words:
- Compound Granule tetrandra /
- Tanshinone IIA /
- TLC /
- HPLC
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