Effect of different density microneedle-roller on transdermal delivery of triamcinolone acetonide
-
摘要: 目的 探讨不同密度滚轮微针对曲安奈德经皮渗透的影响。 方法 采用改良Franz扩散池法考察体外经皮渗透特性,以离体裸鼠皮肤为屏障,对照组、192针、540针滚轮微针处理组分别于2、4、6、8、10、12 h取接收液0.2 ml,用HPLC法测定曲安奈德含量,计算累积渗透量,并测定皮肤内曲安奈德滞留量。采用测定血药浓度法考察在体吸收特性,在体给药2h后HPLC法测定各组皮肤及血浆中曲安奈德的含量。 结果 两种密度滚轮微针对曲安奈德均有不同程度的促透作用。离体透皮实验结果显示,192针微针处理组和540针微针处理组的累积渗透量Q分别是对照组的1.3倍和2.2倍,相应的皮肤内滞留量也分别是对照组的1.9倍和2.8倍。在体实验结果显示,192针微针和540针微针组的皮肤滞留量是对照组的2.1和2.3倍,同时也将血药浓度提高了1.3倍和1.4倍。 结论 两种不同密度滚轮微针均能有效提高曲安奈德的经皮渗透量,提高皮肤内药物含量,同时也导致血药浓度的增加,且不同密度微针的促透作用有所不同。Abstract: Objective To study the effects of different density microneedle-roller arrays on transdermal delivery of triamcinolone acetonide. Methods The in vitro permeation characteristics through isolated nude mouse skin were studied by modified Franz diffusion cells. Levels in the receptor solution of the control group, the 192 needles microneedle rooler group and the 540 needles microneedle rooler group were measured by HPLC at 2、4、6、8、10 and 12 h, and sampling volume was 0.2 ml. The accumulative penetration amount were counted and the level of triamcinolone acetonide in skin were determined. Plasma concentrations of triamcinolone acetonide and skin volume were measured 2 hours later after administration by HPLC to observe the penetration of triamcinolone acetonide in vivo. Results Different density microneedle-roller could promote the transdermal delivery of triamcinolone acetonide respectively. Transdermal experiment results showed that the cumulative infiltration volume of 192 needles group was about 1.3 times of the control samples, and 540 needles was 2.2 times. Accordingly, skin retention volume were1.9times and 2.8 times of the control group respectively. In vivo results showed that skin retention volume were 2.1 times and 2.3 times of control group, while triamcinolone acetonide in plasma were 1.3 times and 1.4 times of control group. Conclusion Microneedles could effectively promote the skin permeability and deposition of triamcinolone acetonide, and triamcinolone acetonide in plasma was increased at the same time. The promoting of different density was diverse.
-
Key words:
- microneedle-roller /
- triamcinolone acetonide /
- percutaneous penetration
-
[1] 黄兴汉,费路华,陈春游.HPLC法测定复方醋酸曲安奈德乳膏的含量[J]. 中国药师,2011,14(1):90. [2] 胡斌,林彩,赵映兰,等.高效液相色谱法测定复方醋酸曲安奈德溶液中醋酸曲安奈德含量[J]. 中国药业,2010,19(21):26. [3] 谢俊,王宇,张晓龙,等.曲安奈德治疗增生性瘢痕的疗效观察[J]. 临床医学与护理研究,2006,5(6):16,33. [4] 董洪峰,刘倩,邹敏. 曲安奈德治疗瘢痕疙瘩28例[J]. 现代临床医学,2006,34(6):443. [5] 潘志斌.微针技术的研究进展[J]. 中国新药与临床杂志,2005,24(11):912. [6] You SK,Noh YW, Park HH,et al. Effect of applying modes of the polymer microneedle-roller on the permeation of L-ascorbic acid in rats[J]. J Drug Target,2010,18(1):15. [7] Zhou CP, Liu YL, Wang HL,et al. Transdermal delivery of insulin using microneedle rollers in vivo[J]. Int J Pharm,2010,392(1-2):127. [8] 胡守莲,范玉峰,李道明.高效液相色谱法测定醋酸曲安奈德尿素乳膏中醋酸曲安奈德的含量[J]. 中国医药导报,2009,6(27):53. [9] 陈浩,孙树茂,秦晓怡,等.高效液相色谱法检测兔血浆中曲安奈德的浓度[J]. 中国医院药学杂志,2009,29(19):1614. [10] Liu W, Hu ML, Liu WS, et al. Investigation of the carbopol gel of solid lipid nanoparticles for the transdermal iontophoretic delivery of triamcinolone acetonide acetate[J]. Int J Pharm,2008, 364:135. [11] Vieira MLT, Singha RP, Derendorf H. Simultaneous HPLC analysis of triamcinolone acetonide and budesonide in microdialysate and rat plasma:Application to a pharmacokinetic study[J]. J Chromatogr B, 2010,878:2967. [12] 邱凯锋,劳贺存,陈露诗,等. 曲安奈德经皮渗透特性研究[J]. 今日药学,2011,21(08):475. -
点击查看大图
计量
- 文章访问数: 2415
- HTML全文浏览量: 262
- PDF下载量: 273
- 被引次数: 0
下载:
