Determination of silybin contents in Gan'an capsule by HPLC
-
摘要: 目的 建立高效液相色谱法测定肝安胶囊中水飞蓟宾的含量。 方法 使用Agilent Zorbax SB-C18柱(4.6 mm×250 mm,5 μm),流动相:1%冰醋酸-甲醇=50:50,流速:1.0 ml/min,柱温:30℃,检测波长:287 nm,测定10批肝安胶囊中水飞蓟宾的含量。 结果 水飞蓟宾在10.28~123.36 μg/ml(r=1)浓度范围内线性关系良好,回收率为97.00%(RSD=0.68%)。10批肝安胶囊中水飞蓟宾含量为11.11~16.65 mg/粒。 结论 该方法简便、准确、重复性好,可以用于肝安胶囊的质量控制。Abstract: Objective To establish a HPLC method for determination of silybin contents in Gan'an capsule. Methods Angilent Zorbax SB C18(4.6 mm×250 mm, 5 μm) column was used with 1% acetic acid:methanol=50:50 as the mobile phase. The flow rate was 1.0 ml/min, and the temperature was at 30℃.The detection wavelength was 287 nm.Determination of silybin contents in Gan'an capsule of 10 batches of preparations. Results The silybin calibration curve was at the range of 10.28~123.36 μg/ml(r=1)with average recovery of 97.00%(RSD=0.68%).10 batches of preparations silybin contents in Gan'an capsule was 11.11~16.65 mg/granule. Conclusion The method was simple accurate and reproducible which could be used for the quality control of Gan'an capsule.
-
Key words:
- HPLC /
- silybin /
- Gan'an capsule /
- determination
-
[1] 任冰如,赵友谊,徐柏衡,等.水飞蓟素提取和水飞蓟宾纯化的研究[J].时珍国医国药,2012,23(3):655. [2] 梁 敏,宋粉云,吕朝霞.HPLC法测定益肝灵片中水飞蓟宾的含量[J].广东药学院学报,2008,24 (5):472. [3] 周采菊,黄华瑞. HPLC法测定水飞蓟素及制剂中水飞蓟宾的含量[J].中国药品标准,2008,9(3):235. [4] Xin RL,Yan FL,Yan XZ,et al. Determination of silymarin in microemulsion by RP-HPLC[J]. J Chin Pharm Sci,2009,18(2):190. [5] 国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社, 2010:76.
计量
- 文章访问数: 2655
- HTML全文浏览量: 303
- PDF下载量: 227
- 被引次数: 0