Anti-hyperlipi-demic effects of polysaccharide MA from Mytilus coruscus on experimental atherosclerosis in rabbits
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摘要: 目的 探讨贻贝多糖MA对实验性动脉粥样硬化兔的降血脂作用及其机制,并观察其对肝脏和主动脉的影响。 方法 采用喂养高脂饲料的方法建立实验性动脉粥样硬化兔模型,连续给予MA 4周后,测定兔血脂水平,如血清三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL)、高密度脂蛋白胆固醇(HDL)水平,以及与血脂代谢相关酶如脂蛋白酯酶(LPL)、肝酯酶(HL)、羟甲基戊二酸单酰辅酶-A还原酶(HMG-CoAR)活性;并进行肝脏的病理组织学和超微结构检查;油红染色观察兔主动脉粥样硬化斑块的脂质沉积情况。 结果 贻贝多糖MA能显著降低实验性动脉粥样硬化兔血清TG、TC、LDL水平,并显著增加HL和 LPL的活性(P<0.05)。MA能减轻肝脏脂质沉积,减少主动脉粥样硬化斑块的脂质沉积和斑块形成。 结论 贻贝多糖MA通过增加HL和 LPL活性发挥调血脂功能,可用于防治家兔动脉粥样硬化和脂肪肝。Abstract: Objective To explore the anti-hyperlipidemic effects and mechanism of polysaccharide MA from Mytilus coruscus in the experimental atherosclerosis rabbits and to observe the effects of MA on liver and aorta. Methods The atherosclerosis rabbit model was established by high-fat diet. The blood lipid such as triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL) in serum and enzymes metabolizing blood lipid such as lipoprteinlipase (LPL), hepaticlipase (HL) and hydroxymethylglutaryl CoA reductase (HMG-CoAR) were measured after atherosclerosis rabbits fed MA for 4 weeks. Histopathology and ultramicroscopic structure of hepatic tissue was observed. Lipid storage in aortic atherosclerotic plaque was observed by the oil red staining method. Results MA significantly reduced the level of TG, TC, LDL in serum and increased HL, LPL (P<0.05). MA reduced lipid storage in liver and aortic atherosclerotic plaque. Conclusion MA might play regulating effects in serum lipid by enhancing the activities of LPL and HL, and inhibit formation of atherosclerosis and fatty liver.
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Key words:
- atherosclerosis /
- Mytilus coruscus /
- polysaccharide /
- blood lipid
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