Rapid identification of chemical components in Artemisiae argyi folium by RRLC-TOFMS
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摘要: 目的 应用快速液相色谱-高分辨飞行时间质谱(RRLC-TOFMS)技术对中药艾叶中化学成分进行快速鉴别。 方法 色谱分离采用 Agilent Eclipse C18色谱柱(2.1 mmü100 mm,1.8 μm);流动相:乙腈(A)-0.1%甲酸水溶液(B),梯度洗脱,A相随时间的变化:5%~30%(0~12 min),30%~80%(12~25 min),80%(25~30 min),进样量 1 μl,流速0.35 ml/min,柱温 40℃。TOFMS、电喷雾离子源(ESI)、正离子模式、质量数扫描范围m/z 100~1 500。 结果 一次性鉴别出艾叶中31种化学成分。 结论 建立基于RRLC-TOFMS技术对艾叶中化学成分快速鉴别的方法,为中药艾叶的质量控制及体内的深入研究奠定基础。Abstract: Objective To identify the chemical components in Artemisiae argyi folium by rapid resolution liquid chromatography-time of flight mass spectrometry (RRLC-TOFMS). Methods The separation was performed on an Agilent Eclipse C18 column (2.1 mmü100 mm,1.8 μm ). The mobile phase consisted of acetonitrile (A) and 0.1% formic acid (B) were in gradient elution.The flowing rate was 0.35 ml/min, the injection volume was 1 μl and the temperature of column was 40℃. Time of flight mass spectrometer (TOFMS) with electro spray ion source (ESI) was applied to qualitative analysis under the positive ion mode,and mass scan range was m/z 100-1 500. Results 31 chemical compounds in Artemisiae argyi folium were identified unequivocally. Conclusion A rapid and efficient RRLC-TOFMS approach for identifying the chemical constituents of Artemisiae argyi folium had been successfully established,which paved a way for quality control and further in vivo studies of Artemisiae argyi folium.
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Key words:
- RRLC /
- Artemisiae argyi folium /
- chemical components /
- TOFMS
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