留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
x 关闭 永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

双配体修饰聚合物泡囊的制备及细胞摄取

解方园,  俞媛,  耿雯倩,  钟延强

downloadPDF
文章导航 > 药学实践与服务  > 2015 >  33(1): 44-48
解方园, 俞媛, 耿雯倩, 钟延强. 双配体修饰聚合物泡囊的制备及细胞摄取[J]. 药学实践与服务, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
引用本文: 解方园, 俞媛, 耿雯倩, 钟延强. 双配体修饰聚合物泡囊的制备及细胞摄取[J]. 药学实践与服务, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
shu
XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
Citation: XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
shu

双配体修饰聚合物泡囊的制备及细胞摄取

doi: 10.3969/j.issn.1006-0111.2015.01.011

  • 第二军医大学药学院药剂学教研室, 上海 200433

基金项目: 国家自然科学基金(81302714);上海市科委纳米专项(11nm0504400)

The preparation and the cell uptake of polymer vesicles modified with dual ligands

  • Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 摘要: 目的 构建一种主动靶向的新型纳米药物载体——聚合物泡囊(polymer vesicles,PVs),并考察其细胞摄取。 方法 以马来酰亚胺-聚乙二醇-聚乳酸-羟基乙酸共聚物(MAL-PEG-PLGA)为载体材料,通过自组装制备PVs,用转铁蛋白(Tf)与Tet-1对PVs进行修饰,构建纳米药物载体(Tf/Tet-1-PVs)。以香豆素-6作为荧光探针包载于药物载体,考察脑微血管内皮细胞(BCEC)及神经细胞(Neuro-2a)对载体系统的摄取。 结果 PVs粒径约80 nm,形态圆整,电镜观察具有明显膜层结构。BCEC细胞和Neuro-2a细胞对Tf/Tet-1-PVs的摄取均显著优于空白对照组和单配体修饰对照组。 结论 PVs经双配体Tf及Tet-1修饰后可促进脑微血管内皮细胞和神经细胞的摄取。
    Abstract: Objective To construct an active targeting drug delivery system- polymer vesicles(PVs),and examined the cellular uptake. Methods Maleimide-polyethylene glycol-poly(lactic-co-glycolic acid)(MAL-PEG-PLGA)was used as carrier materials to prepare PVs by self-assembling.And then PVs was modified by Tf and Tet-1(Tf/Tet-1-PVs).To evaluate its active targeting,coumarin-6 was used as a fluorescent probe to analyze cellular uptake of PVs for both BCEC and Neuro-2a cells. Results PVs was about 80 nm with rounded shape and had obvious film structure.Tf/Tet-1-PVs exhibited a significant role in promoting cellular uptake for both BCEC and Neuro-2a cells compared with control and single ligand-modified group. Conclusion PVs modified with dual ligands could promote the cell uptake for both brain capillary cells and nerve cells.
  • [1] 吴 珍,杜士明,董永成.纳米粒作为脑靶向给药系统的研究进展[J].中国药房,2007,18(13):1029-1031.
    [2] Pardridge WM.Blood-brain barrier drug targeting:the future of brain drug development[J].Mol Interv,2003,3(2):90-105.
    [3] Overmoyer B,Silverman P,Holder LW,et al.Pegylated liposomal doxorubicin and cyclophosphamide as first-line therapy for patients with metastatic or recurrent breast cancer[J].Clin Breast Cancer,2005,6(2):150-157.
    [4] Vorobiof DA, Rapoport BL,Chasen MR,et al.First line therapy with paclitaxel (Taxol) and pegylated liposomal doxorubicin (Caelyx) in patients with metastatic breast cancer:a multicentre phase II study[J].Breast,2004,13(3):219-226.
    [5] Boulikas T.Clinical overview on lipoplatin:a successful liposomal formulation of cisplatin[J].Expert Opin Investig Drugs,2009,18(8):1197-1218.
    [6] Gentile E,Cilurzo F,Marzio LD,et al.Liposomal chemotherapeutics[J].Future Oncol,2013,9(12):1849-1859.
    [7] 陆 彬. 新型给药系统聚合物泡囊及其研究进展(上)[J].中国药师,2009,12(4):439-442,446.
    [8] Cheng JJ,Teply BA,Sherifi I,et al.Formulation of functionalized PLGA-PEG nanoparticles for in vivo targeted drug delivery[J].Biomaterials,2007,28(5):869-876.
    [9] Zhang PC,Hu LJ,Yin Q,et al.Transferrin-modified c[RGDfK]-paclitaxel loaded hybrid micelle for sequential blood-brain barrier penetration and glioma targeting therapy[J].Mol Pharm,2012,9(6):1590-1598.
    [10] Kwon EJ,Lasiene J,Jacobson BE,et al.Targeted nonviral delivery vehicles to neural progenitor cells in the mouse subventricularzone[J].Biomaterials,2010,31(8):2417-2424.
  • [1] 冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军.  雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055
    [2] 马兹芬, 许维恒, 金煜翔, 薛磊.  食管癌的靶向治疗与免疫治疗研究进展 . 药学实践与服务, 2024, 42(6): 231-237. doi: 10.12206/j.issn.2097-2024.202306008
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  2261
  • HTML全文浏览量:  194
  • PDF下载量:  80
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-10-11
  • 修回日期:  2014-11-25

双配体修饰聚合物泡囊的制备及细胞摄取

doi: 10.3969/j.issn.1006-0111.2015.01.011
  • 第二军医大学药学院药剂学教研室, 上海 200433
    • 基金项目:  国家自然科学基金(81302714);上海市科委纳米专项(11nm0504400)
  • 收稿日期: 2014-10-11
  • 修回日期: 2014-11-25

摘要: 目的 构建一种主动靶向的新型纳米药物载体——聚合物泡囊(polymer vesicles,PVs),并考察其细胞摄取。 方法 以马来酰亚胺-聚乙二醇-聚乳酸-羟基乙酸共聚物(MAL-PEG-PLGA)为载体材料,通过自组装制备PVs,用转铁蛋白(Tf)与Tet-1对PVs进行修饰,构建纳米药物载体(Tf/Tet-1-PVs)。以香豆素-6作为荧光探针包载于药物载体,考察脑微血管内皮细胞(BCEC)及神经细胞(Neuro-2a)对载体系统的摄取。 结果 PVs粒径约80 nm,形态圆整,电镜观察具有明显膜层结构。BCEC细胞和Neuro-2a细胞对Tf/Tet-1-PVs的摄取均显著优于空白对照组和单配体修饰对照组。 结论 PVs经双配体Tf及Tet-1修饰后可促进脑微血管内皮细胞和神经细胞的摄取。

English Abstract

解方园, 俞媛, 耿雯倩, 钟延强. 双配体修饰聚合物泡囊的制备及细胞摄取[J]. 药学实践与服务, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
引用本文: 解方园, 俞媛, 耿雯倩, 钟延强. 双配体修饰聚合物泡囊的制备及细胞摄取[J]. 药学实践与服务, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
shu
XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
Citation: XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
shu

    全文HTML

参考文献 (10)

目录

    /

    返回文章
    返回

    版权所有：《药学实践与服务》编辑委员会

    主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

    电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    本系统由 北京仁和汇智信息技术有限公司 开发    百度统计