黎药裸花紫珠在小鼠体内止血活性部位的研究

易博; 张旻; 林海; 陈涛; 徐文彤; 冯世秀

doi:10.3969/j.issn.1006-0111.2015.03.011

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黎药裸花紫珠在小鼠体内止血活性部位的研究

易博, 张旻, 林海, 陈涛, 徐文彤, 冯世秀

文章导航 > 药学实践与服务 > 2015 > 33(3): 235-237,241

易博, 张旻, 林海, 陈涛, 徐文彤, 冯世秀. 黎药裸花紫珠在小鼠体内止血活性部位的研究[J]. 药学实践与服务, 2015, 33(3): 235-237,241. doi: 10.3969/j.issn.1006-0111.2015.03.011

引用本文:

YI Bo, ZHANG Min, LIN Hai, CHEN Tao, XU Wentong, FENG Shixiu. Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 235-237,241. doi: 10.3969/j.issn.1006-0111.2015.03.011

Citation:

YI Bo, ZHANG Min, LIN Hai, CHEN Tao, XU Wentong, FENG Shixiu. Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 235-237,241. doi: 10.3969/j.issn.1006-0111.2015.03.011

黎药裸花紫珠在小鼠体内止血活性部位的研究

doi: 10.3969/j.issn.1006-0111.2015.03.011

易博¹,
张旻²,
林海¹,
陈涛³,
徐文彤^1,,
冯世秀³

1.
解放军187医院药剂科, 海南海口 571159
2.
深圳市中科院仙湖植物园, 深圳市南亚热带植物多样性重点实验室, 广东深圳 518004;西北农林科技大学生命科学学院, 陕西杨凌 712100
3.
深圳市中科院仙湖植物园, 深圳市南亚热带植物多样性重点实验室, 广东深圳 518004

基金项目: 2013年海南省中药现代化专项基金资助(ZY201328)

Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo

1.
Department of Pharmacy, No. 187 Hospital of PLA, Haikou 571159, China
2.
Key Laboratory of Southern Subtropical Plant Diversity, Shenzhen Fairy Lake Botanical Garden, Chinese Academy of Sciences, Shenzhen 518004, China;College of Life Science, Northwest A&F University, Yangling 712100, China
3.
Key Laboratory of Southern Subtropical Plant Diversity, Shenzhen Fairy Lake Botanical Garden, Chinese Academy of Sciences, Shenzhen 518004, China

摘要: 目的确定黎药裸花紫珠的止血活性部位,为其止血活性成分的研究提供依据。方法裸花紫珠经醇提获得醇提物(EtOH-extract)、醇提物经大孔吸附树脂处理分别获得水洗脱物(HP-H₂O-elution)和乙醇洗脱物(HP-EtOH-elution),将EtOH-extract、HP-EtOH-elution和HP-H₂O-elution分别配制成高、中、低剂量的供试药物,云南白药为阳性对照。每日给小鼠灌胃1次,在连续给药7 d后,记录小鼠体质量的变化,采用断尾法和玻片法测定小鼠的出血时间和凝血时间,并以此为筛选指标对各部位止血效果进行评价。结果与模型对照组相比,HP-EtOH-elution对缩短小鼠出血时间的作用效果显著,并且其低剂量组和中剂量组的效果均优于阳性对照组。EtOH-extract、HP-EtOH-elution和HP-H₂O-elution在缩短小鼠的凝血时间和增加小鼠的体质量方面,效果均不显著。结论裸花紫珠的止血活性成分主要集中在醇提物经大孔树脂处理后的乙醇洗脱部位,并且可能是通过影响内源性凝血途径发挥止血作用。
- 裸花紫珠 /
- 活性部位 /
- 出血时间 /
- 凝血时间 /
- 小鼠
Abstract: Objective To screen the hemostatic active extracts from Callicarpa nudiflora. Methods The powdered Callicarpa nudiflora was extracted with 70% EtOH and concentrated to give EtOH-extract. The EtOH-extract was further chromatographed over HP-20 macroporous resin column, eluting with aqua and 95% EtOH to get HP-H₂O-elution and HP-EtOH-elution, respectively. The obtained three extracts of EtOH-extrract, HP-EtOH-elution and HP-H₂O-elution were set as large, middle and small dosage groups for drug preparation, respectively. Yunnan Baiyao was used as a positive control group.The weight increment, bleeding time and clotting time of fed mice were detected by cutting tail and grass slide Methods after intragastric administration for 7 days. Results As compared with blank model and positive control, each dosage groups of HP-EtOH-elution could significantly shortened the bleeding time, of which the small dosage group and middle dosage group even exhibited better Results than the positive control group.Whereas the EtOH-extract, HP-EtOH-elution and HP-H₂O-elution didn't demonstrated significant effect on clotting time as well as the weight increment. Conclusion The HP-EtOH-elution was suggested to be the major active extract possessing hemostatic activity to mice under tested dosages.The hemostatic mechanism may through intrinsic coagulation pathway.This study would be helpful for further phytochemical investigation of C.nudiflora.
- Callicarpa nudiflora /
- active extracts /
- bleeding time /
- clotting time /
- mice

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黎药裸花紫珠在小鼠体内止血活性部位的研究

doi: 10.3969/j.issn.1006-0111.2015.03.011

1. 解放军187医院药剂科, 海南海口 571159

2. 深圳市中科院仙湖植物园, 深圳市南亚热带植物多样性重点实验室, 广东深圳 518004;西北农林科技大学生命科学学院, 陕西杨凌 712100

3. 深圳市中科院仙湖植物园, 深圳市南亚热带植物多样性重点实验室, 广东深圳 518004

基金项目: 2013年海南省中药现代化专项基金资助(ZY201328)

收稿日期: 2013-10-27
修回日期: 2014-05-22

关键词:

摘要: 目的确定黎药裸花紫珠的止血活性部位,为其止血活性成分的研究提供依据。方法裸花紫珠经醇提获得醇提物(EtOH-extract)、醇提物经大孔吸附树脂处理分别获得水洗脱物(HP-H₂O-elution)和乙醇洗脱物(HP-EtOH-elution),将EtOH-extract、HP-EtOH-elution和HP-H₂O-elution分别配制成高、中、低剂量的供试药物,云南白药为阳性对照。每日给小鼠灌胃1次,在连续给药7 d后,记录小鼠体质量的变化,采用断尾法和玻片法测定小鼠的出血时间和凝血时间,并以此为筛选指标对各部位止血效果进行评价。结果与模型对照组相比,HP-EtOH-elution对缩短小鼠出血时间的作用效果显著,并且其低剂量组和中剂量组的效果均优于阳性对照组。EtOH-extract、HP-EtOH-elution和HP-H₂O-elution在缩短小鼠的凝血时间和增加小鼠的体质量方面,效果均不显著。结论裸花紫珠的止血活性成分主要集中在醇提物经大孔树脂处理后的乙醇洗脱部位,并且可能是通过影响内源性凝血途径发挥止血作用。

English Abstract

Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo

1. Department of Pharmacy, No. 187 Hospital of PLA, Haikou 571159, China

2. Key Laboratory of Southern Subtropical Plant Diversity, Shenzhen Fairy Lake Botanical Garden, Chinese Academy of Sciences, Shenzhen 518004, China;College of Life Science, Northwest A&F University, Yangling 712100, China

3. Key Laboratory of Southern Subtropical Plant Diversity, Shenzhen Fairy Lake Botanical Garden, Chinese Academy of Sciences, Shenzhen 518004, China

Received Date: 2013-10-27
Rev Recd Date: 2014-05-22

Keywords:

Abstract: Objective To screen the hemostatic active extracts from Callicarpa nudiflora. Methods The powdered Callicarpa nudiflora was extracted with 70% EtOH and concentrated to give EtOH-extract. The EtOH-extract was further chromatographed over HP-20 macroporous resin column, eluting with aqua and 95% EtOH to get HP-H₂O-elution and HP-EtOH-elution, respectively. The obtained three extracts of EtOH-extrract, HP-EtOH-elution and HP-H₂O-elution were set as large, middle and small dosage groups for drug preparation, respectively. Yunnan Baiyao was used as a positive control group.The weight increment, bleeding time and clotting time of fed mice were detected by cutting tail and grass slide Methods after intragastric administration for 7 days. Results As compared with blank model and positive control, each dosage groups of HP-EtOH-elution could significantly shortened the bleeding time, of which the small dosage group and middle dosage group even exhibited better Results than the positive control group.Whereas the EtOH-extract, HP-EtOH-elution and HP-H₂O-elution didn't demonstrated significant effect on clotting time as well as the weight increment. Conclusion The HP-EtOH-elution was suggested to be the major active extract possessing hemostatic activity to mice under tested dosages.The hemostatic mechanism may through intrinsic coagulation pathway.This study would be helpful for further phytochemical investigation of C.nudiflora.