留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
x 关闭 永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

慢性不可预见性温和应激致抑郁对大鼠肝脏转运多肽mRNA和蛋白表达的影响

刘萃萃,  肖志军,  陆赛花,  徐峰

downloadPDF
文章导航 > 药学实践与服务  > 2019 >  37(2): 121-125
刘萃萃, 肖志军, 陆赛花, 徐峰. 慢性不可预见性温和应激致抑郁对大鼠肝脏转运多肽mRNA和蛋白表达的影响[J]. 药学实践与服务, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
引用本文: 刘萃萃, 肖志军, 陆赛花, 徐峰. 慢性不可预见性温和应激致抑郁对大鼠肝脏转运多肽mRNA和蛋白表达的影响[J]. 药学实践与服务, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
shu
LIU Cuicui, XIAO Zhijun, LU Saihua, XU Feng. Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
Citation: LIU Cuicui, XIAO Zhijun, LU Saihua, XU Feng. Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
shu

慢性不可预见性温和应激致抑郁对大鼠肝脏转运多肽mRNA和蛋白表达的影响

doi: 10.3969/j.issn.1006-0111.2019.02.005
  • 1.

    上海健康医学院附属第六人民医院南院, 上海 201499

  • 2.

    上海交通大学附属第六人民医院南院, 上海 201499

基金项目: 上海健康医学院种子基金项目（HMSF-16-22-023，SFP-18-20-15-002）；上海奉贤区科委科学技术发展基金（20161008）；上海市卫计委科研课题（20154Y0123）

Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats

  • 1.

    Shanghai University of Medicine & Health Sciences Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China

  • 2.

    Shanghai Jiaotong University Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China

  • 摘要: 目的 研究慢性不可预见性温和应激（chronic unpredicted mild stress，CUMS）致抑郁对大鼠肝脏有机阴离子转运多肽（organic anion transporting polypeptides，Oatps） mRNA和蛋白表达的影响。 方法 24只雄性SD大鼠随机分为抑郁模型组和对照组，模型组连续8周给予CUMS，对照组正常饲养。分别于造模前后称量体重、进行糖水偏好实验和旷场实验，并测定大鼠血浆去甲肾上腺素和皮质酮水平以验证抑郁模型的建立。采用实时荧光定量聚合酶链式反应（RT-qPCR）和蛋白质印迹法（Western blotting）分别检测大鼠肝脏Oatp1a1、Oatp1a4和Oatp1b2 mRNA和蛋白表达。 结果 与对照组相比，模型组大鼠体重减轻，糖水偏好百分比下降，垂直运动得分和水平运动得分减少，血浆去甲肾上腺素和皮质酮水平升高，肝脏Oatp1a1、Oatp1a4和Oatp1b2 mRNA和蛋白表达下降。 结论 CUMS致抑郁下调大鼠肝脏Oatps mRNA和蛋白表达。
    Abstract: Objective To explore the impact of chronic unpredicted mild stress (CUMS)induced depression on mRNA and protein expression of liver organic anion transporting polypeptides (Oatps) in rats. Methods Twenty-four male SD rats were divided into depression group and control group.Rats in depression group were exposed CUMS for 8 weeks consecutively.Weight,open-field test,sucrose preference,plasma concentration of noradrenaline and corticosterone were assayed before and after model establishment to evaluate the depression.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were measured by RT-qPCR and Western blotting,respectively. Results In CUMS induced depression group,weight,sucrose consumption,vertical score and horizontal score were decreased while levels of corticosterone and norepinephrine were increased compared with control group.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were reduced compared with control group. Conclusion The mRNA and protein expression of liver Oatps in rats were regulated down by CUMS induced depression.
  • [1] KOVACSICS D,PATIK I,ÖZVEGY-LACZKA C.The role of organic anion transporting polypeptides in drug absorption,distribution,excretion and drug-drug interactions[J].Expert Opin Drug Metab Toxicol,2017,13(4):409-424.
    [2] HAGENBUCH B,MEIER P J.Organic anion transporting polypeptides of the OATP/SLC21 family:phylogenetic classification as OATP/SLCO superfamily,new nomenclature and molecular/functional properties[J].Pflugers Arch,2004,447(5):653-665.
    [3] WEI H,ZHOU T,TAN B,et al. Impact of chronic unpredicted mild stress-induced depression on repaglinide fate via glucocorticoid signaling pathway[J].Oncotarget,2017,8(27):44351-44365.
    [4] XIA Z,WEI H,DUAN J,et al. Chronic unpredicted mild stress-induced depression alter saxagliptin pharmacokinetics and CYP450 activity in GK rats[J].PeerJ,2016,4:e1611.
    [5] ZENG Y,XIE X,DUAN J,et al. Perturbation of mitiglinide metabolism by chronic unpredicted mild stress in rats[J].Sci Rep,2014,4:3794.
    [6] YANG L,ZHAO Y,WANG Y,et al. The Effects of psychological stress on depression[J].Curr Neuropharmacol,2015,13(4):494-504.
    [7] WU G F,REN S,TANG R Y,et al. Antidepressant effect of taurine in chronic unpredictable mild stress-induced depressive rats[J].Sci Rep,2017,7(1):4989.
    [8] WANG B,WANG Y,WU Q,et al. Effects of α2A Adrenoceptors on Norepinephrine Secretion from the Locus Coeruleus during Chronic Stress-Induced Depression[J].Front Neurosci,2017,11:243.
    [9] GONG I Y,KIM R B.Impact of genetic variation in OATP transporters to drug disposition and response[J].Drug Metab Pharmacokinet,2013,28(1):4-18.
    [10] HAGENBUCH B,STIEGER B.The SLCO (former SLC21) superfamily of transporters[J].Mol Aspects Med,2013,34(2-3):396-412.
    [11] HARTMANN G,CHEUNG A K,PIQUETTE-MILLER M.Inflammatory cytokines,but not bile acids,regulate expression of murine hepatic anion transporters in endotoxemia[J].J Pharmacol Exp Ther,2002,303(1):273-281.
    [12] HAYLEY S.Toward an anti-inflammatory strategy for depression[J].Front Behav Neurosci,2011,5:19.
    [13] YOU Z,LUO C,ZHANG W,et al. Pro-and anti-inflammatory cytokines expression in rat's brain and spleen exposed to chronic mild stress:involvement in depression[J].Behav Brain Res,2011,225(1):135-141.
  • [1] 唐淑慧, 凤美娟, 薛智霞, 鲁桂华.  帕博利珠单抗治疗所致免疫相关不良反应与中医体质的相关性研究 . 药学实践与服务, 2024, 42(5): 217-222. doi: 10.12206/j.issn.2097-2024.202311029
    [2] 张晶晶, 索丽娜, 郑兆红.  89例细菌性肝脓肿的临床特征及抗感染治疗分析 . 药学实践与服务, 2024, 42(6): 267-272. doi: 10.12206/j.issn.2097-2024.202302039
    [3] 修建平, 杨朝爱, 刘禧澳, 潘乾禹, 韦广旭, 王卫星.  全反式维甲酸对肝星状细胞活化及氧化应激的作用和机制探索 . 药学实践与服务, 2024, 42(7): 1-6. doi: 10.12206/j.issn.2097-2024.202312054
    [4] 景凯, 杨慈荣, 张圳, 臧艺蓓, 刘霞.  黄芪甲苷衍生物治疗慢性心力衰竭小鼠的药效评价及作用机制研究 . 药学实践与服务, 2024, 42(5): 190-197. doi: 10.12206/j.issn.2097-2024.202310004
    [5] 王耀振, 徐灿, 吕顺莉, 田泾, 张东炜.  钾离子竞争性酸阻滞剂的药学特征研究进展 . 药学实践与服务, 2024, 42(7): 1-7. doi: 10.12206/j.issn.2097-2024.202306040
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  2501
  • HTML全文浏览量:  372
  • PDF下载量:  709
  • 被引次数: 0
出版历程
  • 收稿日期:  2018-06-11
  • 修回日期:  2018-10-15

慢性不可预见性温和应激致抑郁对大鼠肝脏转运多肽mRNA和蛋白表达的影响

doi: 10.3969/j.issn.1006-0111.2019.02.005
  • 1. 上海健康医学院附属第六人民医院南院, 上海 201499
  • 2. 上海交通大学附属第六人民医院南院, 上海 201499
    • 基金项目:  上海健康医学院种子基金项目（HMSF-16-22-023，SFP-18-20-15-002）；上海奉贤区科委科学技术发展基金（20161008）；上海市卫计委科研课题（20154Y0123）
  • 收稿日期: 2018-06-11
  • 修回日期: 2018-10-15

摘要: 目的 研究慢性不可预见性温和应激（chronic unpredicted mild stress，CUMS）致抑郁对大鼠肝脏有机阴离子转运多肽（organic anion transporting polypeptides，Oatps） mRNA和蛋白表达的影响。 方法 24只雄性SD大鼠随机分为抑郁模型组和对照组，模型组连续8周给予CUMS，对照组正常饲养。分别于造模前后称量体重、进行糖水偏好实验和旷场实验，并测定大鼠血浆去甲肾上腺素和皮质酮水平以验证抑郁模型的建立。采用实时荧光定量聚合酶链式反应（RT-qPCR）和蛋白质印迹法（Western blotting）分别检测大鼠肝脏Oatp1a1、Oatp1a4和Oatp1b2 mRNA和蛋白表达。 结果 与对照组相比，模型组大鼠体重减轻，糖水偏好百分比下降，垂直运动得分和水平运动得分减少，血浆去甲肾上腺素和皮质酮水平升高，肝脏Oatp1a1、Oatp1a4和Oatp1b2 mRNA和蛋白表达下降。 结论 CUMS致抑郁下调大鼠肝脏Oatps mRNA和蛋白表达。

English Abstract

刘萃萃, 肖志军, 陆赛花, 徐峰. 慢性不可预见性温和应激致抑郁对大鼠肝脏转运多肽mRNA和蛋白表达的影响[J]. 药学实践与服务, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
引用本文: 刘萃萃, 肖志军, 陆赛花, 徐峰. 慢性不可预见性温和应激致抑郁对大鼠肝脏转运多肽mRNA和蛋白表达的影响[J]. 药学实践与服务, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
shu
LIU Cuicui, XIAO Zhijun, LU Saihua, XU Feng. Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
Citation: LIU Cuicui, XIAO Zhijun, LU Saihua, XU Feng. Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
shu

    全文HTML

参考文献 (13)

目录

    /

    返回文章
    返回

    版权所有：《药学实践与服务》编辑委员会

    主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

    电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    本系统由 北京仁和汇智信息技术有限公司 开发    百度统计