Protective effect and mechanism of sodium dichloroacetate on BV2 cells damaged by OGD
-
摘要: 目的 研究二氯乙酸钠（dichloroacetate，DCA）对氧糖剥夺（oxygen glucose deprivation，OGD）损伤模型中小鼠小胶质细胞（BV2细胞）的保护作用，并探讨其作用机制。 方法 将BV2细胞分为3组：对照组、OGD组、DCA治疗组，通过OGD 4 h建立损伤模型。CCK-8和流式细胞仪检测细胞凋亡及ROS和NO的表达，Western blot检测NF-κB通路相关蛋白表达水平。 结果 CCK-8及流式细胞检测结果表明，DCA可显著降低OGD诱导的BV2细胞凋亡，并且减少OGD后细胞中活性氧（ROS）和一氧化氮（NO）的表达（P<0.05）。Western blot结果显示，DCA可显著影响OGD损伤介导的BV2细胞JNK、I-κB和NF-κB蛋白表达水平（P<0.05）。 结论 DCA对OGD损伤的BV2细胞具有保护作用，其机制与抗凋亡、抗氧化和抗炎作用有关。Abstract: Objective To study the protective effect and mechanism of dichloroacetate (DCA) on BV2 microglia in oxygen glucose deprivation (OGD) injury model. Methods BV2 cells were divided into 3 groups:CON group,OGD group and DCA intervention group.The injury model was established by OGD 4 h.CCK-8 and flow cytometry was used to detect apoptosis and expression of ROS and NO in cells.Western blot was used to detect the expression of proteins related to NF-κB pathway. Results CCK-8 and flow cytometry showed that the apoptosis of BV2 cells induced by OGD and the expression of ROS and NO in OGD cells were decreased significantly by DCA (P<0.05).Western blot analysis showed that the expression of I-κB,JNK and NF-κB protein in BV2 cells mediated by OGD injury were influenced significantly by DCA (P<0.05). Conclusion DCA had a protective effect on OGD-injured BV2 cells through anti-oxidation and anti-apoptosis.
-
Key words:
- dichloroacetate /
- OGD /
- ROS /
- NF-κB
-
[1] MOZAFFARIAN D,BENJAMIN E J,GO A S,et al.Heart disease and stroke statistics-2015 update:a report from the American Heart Association[J].Circulation,2015,131(4):e29-322. [2] JIN R,YANG G,LI G.Inflammatory mechanisms in ischemic stroke:role of inflammatory cells[J].J Leukoc Biol,2010,87(5)::779-789. [3] LAMKANFI M,DIXIT V M.Mechanisms and functions of inflammasomes[J].Cell,2014,157(5):1013-1022. [4] IADECOLA C,ANRATHER J.The immunology of stroke:from mechanisms to translation[J].Nat Med,2011,17(7):796-808. [5] MICHELAKIS E D,SUTENDRA G,DROMPARIS P,et al.Metabolic modulation of glioblastoma with dichloroacetate[J].Sci Transl Med,2010,2(31):31-34. [6] DEUSE T,HUA X,WANG D,et al.Dichloroacetate prevents restenosis in preclinical animal models of vessel injury[J].Nature,2014,509(7502):641-644. [7] HONG D K,KHO A R,CHOI B Y,et al.Combined treatment with dichloroacetic acid and pyruvate reduces hippocampal neuronal death after transient cerebral ischemia[J].Front Neurol,2018,9:137. [8] 洪倩,王实,陈昌秀,等.血塞通注射液对体外OGD/R损伤的BV2细胞炎症反应的影响[J].中国中药杂志,2017,42(1):140-145. [9] FAGAN S C,WALLER J L,NICHOLS F T,et al.Minocycline to improve neurologic outcome in stroke (MINOS):a dose-finding study[J].Stroke,2010,41(10):2283-2287. [10] YANG Y,SUN Y,CHEN J,et al.AKT-independent activation of p38 MAP kinase promotes vascular calcification[J].Redox Biol,2018,16:97-103. [11] TAKPP,FIRESTEIN G S.NF-kappaB:a key role in inflammatory diseases[J].J Clin Invest,2001,107(1):7-11. [12] 崔春英,申超,洪艳,等.柿叶黄酮提取物抑制BV2小胶质细胞活化在体外氧糖剥夺/再灌注损伤模型中发挥抗炎作用的研究[J].免疫学杂志,2018,34(7):593-599. -
点击查看大图
计量
- 文章访问数: 3408
- HTML全文浏览量: 405
- PDF下载量: 375
- 被引次数: 0
下载:
