Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC
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摘要: 目的 建立同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量的方法。 方法 采用高效液相色谱法。色谱柱为Agilent Extend-C18（4.6 mm×150 mm，5 μm），流动相为甲醇-乙酸铵缓冲溶液（20 mmol/L，pH=7.5），梯度洗脱，甲醇比例随时间变化为：0~10 min、甲醇→60%；10~12 min、甲醇60%→92%；12~20 min、甲醇→92%，采集时间20 min，平衡时间10 min，流速1 ml/min，紫外检测波长218 nm，柱温25℃，进样量15 μl。 结果 3种成分在30 min内基线分离，方法学验证表明，线性关系良好（r>0.9995），日内日间精密度均小于3.0%，回收率在90%~108%之间，稳定性和重复性RSD均<3.0%，符合方法学要求。按照新建立的方法测定了3个批次样品中3种成分的含量，结果符合要求。 结论 本方法简便可靠，可为多效防护霜的质量控制提供依据，也为其质量标准研究奠定了基础。Abstract: Objective To establish a HPLC method for the simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream. Methods The HPLC condition was as follows.The column was Agilent Extend-C18 (4.6 mm×150 mm,5 μm).The mobile phase was methanol and ammonium acetate buffer solution (20 mmol/L,pH=7.5).Gradient elution.The methanol ratio varies with time as follows:0-10 min,methanol→60%; 10-12 min,methanol 60%→92%;12-20 min,methanol→92%.Acquisition time was 20 minutes.Liquid phase equilibrium time was 10 min.Flow rate was 1 ml/min.UV detection wavelength was 218 nm.Column temperature was 25℃.Injection volume was 15 μl. Results The three components were separated at baseline within 30 min.The methodological verification showed that the standard curves had good linearity (r>0.9995).The inter-day precision were less than 3.0%.The recovery was between 90% and 108%.The RSD% of stability and repeatability were also less than 3.0%.The results showed that the method could meet the requirements of method validation.The contents of the three components in three batches were determined according to the newly established method. Conclusion The method is simple and reliable,can provide a basis for the quality control of multi-effect protective cream,and laid a foundation for its quality standard research.
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[1] 刘鹏,蔡杨.某特种部队海练期间伤病情况分析及对策[J].海军医学杂志,2016,37(4):296-298. [2] 卢畅,费文超,纪世召,等.海军某士官学校学员基层部队服役期间训练伤发生情况调查[J].解放军预防医学杂志,2017,35(1):16-18. [3] Lotan A.Method for inhibiting nematocyst discharge:US,US6406709[P].2002. [4] 张智.水母蜇伤皮肤防护剂研究[D].上海:第二军医大学,2010. [5] Ginestar G J,Panyella C D,Catalá E R,et al.Anti-jellyfish combinations:,EP 2380577 A1[P].2011. [6] 陈云霞,马强,白桦,等.祛痘化妆品中苯海拉明的高效液相色谱检测及质谱确证[J].分析科学学报,2014,30(4):521-524. [7] 肖庚鹏,方永培,王水生,等.化妆品中甲氧基肉桂酸辛酯和水杨酸乙基己酯含量的测定[J].日用化学工业,2009,39(1):68-70. [8] 徐惠金,林峰,吴映璇.高效液相色谱法测定化妆品中9种防晒剂[J].理化检验-化学分册,2015,51(5):621-624. -
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