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适配体C2min介导的可靶向2种前列腺癌基因的递送系统

张晶,  顾永卫,  武鑫

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文章导航 > 药学实践与服务  > 2020 >  38(1): 47-51,66
张晶, 顾永卫, 武鑫. 适配体C2min介导的可靶向2种前列腺癌基因的递送系统[J]. 药学实践与服务, 2020, 38(1): 47-51,66. doi: 10.3969/j.issn.1006-0111.201906038
引用本文: 张晶, 顾永卫, 武鑫. 适配体C2min介导的可靶向2种前列腺癌基因的递送系统[J]. 药学实践与服务, 2020, 38(1): 47-51,66. doi: 10.3969/j.issn.1006-0111.201906038
shu
ZHANG Jing, GU Yongwei, WU Xin. The C2min aptamer-modified gene delivery system for targeting ADPC/AIPC prostate cancer[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 47-51,66. doi: 10.3969/j.issn.1006-0111.201906038
Citation: ZHANG Jing, GU Yongwei, WU Xin. The C2min aptamer-modified gene delivery system for targeting ADPC/AIPC prostate cancer[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 47-51,66. doi: 10.3969/j.issn.1006-0111.201906038
shu

适配体C2min介导的可靶向2种前列腺癌基因的递送系统

doi: 10.3969/j.issn.1006-0111.201906038
  • 1.

    内蒙古医科大学附属医院药剂部, 内蒙古 呼和浩特 010050

  • 2.

    上海维洱实验室, 上海 201712

  • 3.

    内蒙古医科大学附属医院药剂部, 内蒙古 呼和浩特 010050;上海维洱实验室, 上海 201712

基金项目: 国家自然科学基金（81072100）；上海市青年科技启明星计划资助（18QB1400400）

The C2min aptamer-modified gene delivery system for targeting ADPC/AIPC prostate cancer

  • 1.

    Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China

  • 2.

    Shanghai Wei Er Laboratory, shanghai 201712, China

  • 3.

    Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China;Shanghai Wei Er Laboratory, shanghai 201712, China

  • 摘要: 目的 通过合成可靶向两种前列腺癌的基因载体PAMAM-PEG-C2min，以提高基因的转染效率和肿瘤靶向性。 方法 将双功能聚乙二醇的一端与聚酰胺-胺（PAMAM）相连，另一端与适配体（C2min）连接，并利用1H NMR技术对合成的PAMAM-PEG-C2min基因载体进行结构鉴定。通过两种前列腺癌PC3和LNCaP细胞的体外摄取和基因转染实验（包载siR-M基因），考察纳米复合物的生物学特性。并利用动物活体成像技术考察合成的纳米复合物的体内分布特征。 结果 核磁共振结果表明，本研究成功合成了PAMAM-PEG-C2min。PC3和LNCaP细胞对PAMAM-PEG-C2min的摄取结果体现出浓度依赖性。且与不经C2min修饰的PAMA-PEGM相比，PAMAM-PEG-C2min递药系统的基因转染效率和肿瘤细胞靶向性明显提高。体内靶向性结果表明，PAMAM-PEG-C2min可实现同时靶向2种前列腺癌组织的作用。 结论 本研究合成的PAMAM-PEG-C2min递送载体具有良好的肿瘤靶向性，为前列腺癌的综合治疗和靶向治疗提供了新的技术平台。
    Abstract: Objective To synthesize a novel prostate cancer targeting gene vector PAMAM-PEG-C2min and improve gene transfection efficiency targeting on prostate cancer. Methods The aptamer (C2min) and polyamide-amine (PAMAM) were ligated by polyethylene glycol (PEG). The structure of the synthesized PAMAM-PEG-C2min was identified by NMR. The biological characteristics of the nanoparticles were examined by the uptake experiments and gene transfection experiments (the loaded gene was siR-M) with the prostate cancer cells (PC3 and LNCaP). Besides, the in vivo targeting was investigated using in vivo image system. The in vivo targeting results indicated that PAMAM-PEG-C2min can achieve the simultaneous targeting of two prostate cancer tissues. Results The PAMAM-PEG-C2min synthesis was confirmed by NMR. Cell uptake experiments showed that the cell uptake efficiency of PAMAM-PEG-C2min was concentration dependent. In vitro experiments showed that the PC3 and LNCaP cells transfection efficiency and targeting of PAMAM-PEG modified with C2min were significantly improved compared with the PEG modified PAMAM. Conclusion PAMAM-PEG-C2min is a potential targeted drug delivery vehicle. It provides a new technology platform for comprehensive and specific targeting treatment of prostate cancer.
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  • 收稿日期:  2019-06-14
  • 修回日期:  2019-07-26

适配体C2min介导的可靶向2种前列腺癌基因的递送系统

doi: 10.3969/j.issn.1006-0111.201906038
  • 1. 内蒙古医科大学附属医院药剂部, 内蒙古 呼和浩特 010050
  • 2. 上海维洱实验室, 上海 201712
  • 3. 内蒙古医科大学附属医院药剂部, 内蒙古 呼和浩特 010050;上海维洱实验室, 上海 201712
    • 基金项目:  国家自然科学基金（81072100）；上海市青年科技启明星计划资助（18QB1400400）
  • 收稿日期: 2019-06-14
  • 修回日期: 2019-07-26

摘要: 目的 通过合成可靶向两种前列腺癌的基因载体PAMAM-PEG-C2min，以提高基因的转染效率和肿瘤靶向性。 方法 将双功能聚乙二醇的一端与聚酰胺-胺（PAMAM）相连，另一端与适配体（C2min）连接，并利用1H NMR技术对合成的PAMAM-PEG-C2min基因载体进行结构鉴定。通过两种前列腺癌PC3和LNCaP细胞的体外摄取和基因转染实验（包载siR-M基因），考察纳米复合物的生物学特性。并利用动物活体成像技术考察合成的纳米复合物的体内分布特征。 结果 核磁共振结果表明，本研究成功合成了PAMAM-PEG-C2min。PC3和LNCaP细胞对PAMAM-PEG-C2min的摄取结果体现出浓度依赖性。且与不经C2min修饰的PAMA-PEGM相比，PAMAM-PEG-C2min递药系统的基因转染效率和肿瘤细胞靶向性明显提高。体内靶向性结果表明，PAMAM-PEG-C2min可实现同时靶向2种前列腺癌组织的作用。 结论 本研究合成的PAMAM-PEG-C2min递送载体具有良好的肿瘤靶向性，为前列腺癌的综合治疗和靶向治疗提供了新的技术平台。

English Abstract

张晶, 顾永卫, 武鑫. 适配体C2min介导的可靶向2种前列腺癌基因的递送系统[J]. 药学实践与服务, 2020, 38(1): 47-51,66. doi: 10.3969/j.issn.1006-0111.201906038
引用本文: 张晶, 顾永卫, 武鑫. 适配体C2min介导的可靶向2种前列腺癌基因的递送系统[J]. 药学实践与服务, 2020, 38(1): 47-51,66. doi: 10.3969/j.issn.1006-0111.201906038
shu
ZHANG Jing, GU Yongwei, WU Xin. The C2min aptamer-modified gene delivery system for targeting ADPC/AIPC prostate cancer[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 47-51,66. doi: 10.3969/j.issn.1006-0111.201906038
Citation: ZHANG Jing, GU Yongwei, WU Xin. The C2min aptamer-modified gene delivery system for targeting ADPC/AIPC prostate cancer[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 47-51,66. doi: 10.3969/j.issn.1006-0111.201906038
shu

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