反相高效液相色谱法测定毛鱼藤中鱼藤酮的含量
Determination of rotenone in Derris elliptica by reversed-phase high performance liquid chromatography
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摘要: 目的:建立以反相高效液相色谱法测定毛鱼藤中鱼藤酮含量的方法。方法:以ZORBAX Eclipse XDB-C18（5μm,4.6×150mm）色谱柱为固定相;乙腈:0.1%磷酸溶液（48:52,v/v,pH2.6）为流动相;流速1.0mL/min;柱温40.0℃;检测波长299nm。结果:鱼藤酮在浓度为30-50mg/L范围内,其峰面积与浓度线性关系良好,线性方程为A=20.7311C-0.3616（r=0.9998）,平均加样回收率为100.74%,RSD为0.86%。结论:本方法操作简便、快速,结果准确,可用于毛鱼藤中鱼藤酮的含量测定。Abstract: Objective:To establish a reversed-phase high performance liquid chromatography method for rotenone in Derris elliptica.Methods:The determination was performed on ZORBAX Eclipse XDB-C18 (5 μm,4.6×150 mm)column. The mobile phase consisted of acetonitrile and 0.1% phosphate acid solution(4852,v/v,pH 2.6)with flow rate at 1.0 mL/min and determination wavelength at 299 nm under 40.0 ℃.Results:Under the conditions described, the calibration curve of rotenone was linear over the concentration range of 30 to 50 mg/L. The regression equation was: A=20.731 1C-0.361 6, r=0.999 8(n=5,P<0.01). The average recovery for rotenone was 100.74%. The mean relative standard deviation(RSD) was 0.86%.Conclusion:The method is simple and accurate, and suitable for the determination of rotenone in Derris elliptica.
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