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用HPLC法测定丹参素棕榈醇酯脂质体药物含量

王晶,  张川,  栗意,  任金妹,  唐扣明

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文章导航 > 药学实践与服务  > 2019 >  37(5): 450-452,480
王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
引用本文: 王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
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WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
Citation: WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
shu

用HPLC法测定丹参素棕榈醇酯脂质体药物含量

doi: 10.3969/j.issn.1006-0111.2019.05.012
  • 1.

    复旦大学附属中山医院青浦分院药剂科, 上海 201700

  • 2.

    上海大学医学院, 上海 200003

  • 3.

    上海健康医学院附属周浦医院, 上海 201318

基金项目: 上海市卫生和计划生育委员会科研项目资助（20164Y0101）

Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method

  • 1.

    Department of Pharmacy, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai 201700, China

  • 2.

    Department of Medicine, Shanghai University, Shanghai 200003, China

  • 3.

    Zhoupu Hospital Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai 201318, China

  • 摘要: 目的 建立HPLC法测定丹参素棕榈醇酯脂质体中药物含量的方法。 方法 采用Agilent ZORBAX Eclipse Plus C18柱（4.6 mm×200 mm，5 μm）；以乙腈-水（50∶50）含0.02%甲酸和0.02%三乙胺水、以pH 6~7的溶液为流动相，流速为1 ml/min，检测波长为280 nm，柱温为室温。 结果 本色谱条件下丹参素棕榈醇酯与辅料及溶剂分离度符合要求，在0.05~0.45 mg/ml范围内线性关系良好（r=0.999 5，n=5），回收率在96%~102%之间，日内及日间RSD均小于2%（n=5）。3批样品的含量分别为97.81%、101.20%、98.53%。 结论 该方法准确可靠、简单快速，可用于丹参素棕榈醇酯脂质体含量的测定。
    Abstract: Objective To establish a HPLC method for the assay of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate (PDSS) in liposomes. Methods The separation was performed with an Agilent ZORBAX Eclipse Plus C18 column (4.6 mm×200 mm,5 μm) at room temperature.The mobile phase was acetonitrile-aqueous solution (500 ml water + 0.1 ml triethylamine + 0.1 ml formic acid,pH=6-7) (50:50) with the flow rate of 1 ml/min.Zidovudine palmitate was detected at 280 nm. Results PDSS had a good linear recovery between 0.05-0.45 mg/ml.The intra-day and inter-day RSD were <2%.The extracted recovery was in the range of 96% to 102%.The assay results for three batches were 97.81%,101.20% and 98.53%. Conclusion This method is simple,accurate and reliable.It can be used for the determination of PDSS in liposomes.
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  • 收稿日期:  2019-02-14
  • 修回日期:  2019-05-06

用HPLC法测定丹参素棕榈醇酯脂质体药物含量

doi: 10.3969/j.issn.1006-0111.2019.05.012
  • 1. 复旦大学附属中山医院青浦分院药剂科, 上海 201700
  • 2. 上海大学医学院, 上海 200003
  • 3. 上海健康医学院附属周浦医院, 上海 201318
    • 基金项目:  上海市卫生和计划生育委员会科研项目资助（20164Y0101）
  • 收稿日期: 2019-02-14
  • 修回日期: 2019-05-06

摘要: 目的 建立HPLC法测定丹参素棕榈醇酯脂质体中药物含量的方法。 方法 采用Agilent ZORBAX Eclipse Plus C18柱（4.6 mm×200 mm，5 μm）；以乙腈-水（50∶50）含0.02%甲酸和0.02%三乙胺水、以pH 6~7的溶液为流动相，流速为1 ml/min，检测波长为280 nm，柱温为室温。 结果 本色谱条件下丹参素棕榈醇酯与辅料及溶剂分离度符合要求，在0.05~0.45 mg/ml范围内线性关系良好（r=0.999 5，n=5），回收率在96%~102%之间，日内及日间RSD均小于2%（n=5）。3批样品的含量分别为97.81%、101.20%、98.53%。 结论 该方法准确可靠、简单快速，可用于丹参素棕榈醇酯脂质体含量的测定。

English Abstract

王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
引用本文: 王晶, 张川, 栗意, 任金妹, 唐扣明. 用HPLC法测定丹参素棕榈醇酯脂质体药物含量[J]. 药学实践与服务, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
shu
WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
Citation: WANG Jing, ZHANG Chuan, LI Yi, REN Jinmei, TANG Kouming. Determination of palmitul 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate liposome by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 450-452,480. doi: 10.3969/j.issn.1006-0111.2019.05.012
shu

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