GUO Li-an, WANG Shu-ping, XIANG Li, JIANG Peng, ZHANG Wei-dong, LIU Run-hui. Identification of volatility components of Shexiang Baoxin pill in rat plasma by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(3): 207-210. doi: 10.3969/j.issn.1006-0111.2012.03.013
| Citation:
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GUO Li-an, WANG Shu-ping, XIANG Li, JIANG Peng, ZHANG Wei-dong, LIU Run-hui. Identification of volatility components of Shexiang Baoxin pill in rat plasma by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(3): 207-210. doi: 10.3969/j.issn.1006-0111.2012.03.013
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Identification of volatility components of Shexiang Baoxin pill in rat plasma by GC-MS
- 1.
Second Military Medical University, Training Department, Shanghai 200433,China
- 2.
School of Pharmacy, Second Military Medical University, Shanghai 200433,China
- Received Date: 2012-04-16
- Rev Recd Date:
2012-05-09
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Abstract
Objective To identify the volatile components in rat serum of Shexiang Baoxin Pill (SBP) by gas chromatography couples with mass spectrometry (GC-MS). Methods Serum samples from control and SBP administration rats were deproteinated by adding methanol prior to gas chromatography. The separation was carried out on a Thermo TR-5 ms column (30 m×0.25 mm I.D.). The inlet temperature was set at 220 ℃ and ion source temperature was 250 ℃ with gradient warming. The carrier gas was N2 with a gas flow rate of 1.0 ml/min, and the injection volume was 1μl. The total analysis time was 59min. The data acquisition were performed on Xcalibur workstation. Results Ten components including 6 components from SBP and 4 metabolites were observed from a comprehensive analysis of the chromatography of SBP solution. All the observed components were identified by using the online NIST05 library. Conclusion An integrated method for screening volatile bioactive components in serum after oral administration of SBP was established, which provided helpful information for further mechanism research.
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