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| Citation:
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XU Xue-jun, TAO Qing, XU De-qin. Determination of fluoxetine hydrochloride in plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(3): 222-225. doi: 10.3969/j.issn.1006-0111.2012.03.019
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Determination of fluoxetine hydrochloride in plasma by HPLC
- 1.
Department of Pharmacy, Anhui General Hospital of the Chinese People's Armed Police Forces, Hefei 230061, China
- 2.
Department of Pharmacy ,The 108th Hospital of PLA, Shanghai 200336,China
- Received Date: 2012-02-10
- Rev Recd Date:
2012-03-27
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Abstract
Objective To establish and optimize a simple, rapid, reliable, high sensitive and high selective method for the determination of fluoxetine hydrochloride in plasma by high-performance liquid chromatographic (HPLC) so as to monitor the clinical drug use. Methods The plasma samples were deproteined by acetonitrile. Separation was performed on a Hypersil ODS2 column(5 μm, 4.6 mm×200 mm). The mobile phase contained acetonitrile-25 mmol/ L phosphate buffer(pH6.5)(34:66). The flow rate and sample volume injected were 1.2 ml/min and 20 μl, respectively. Detection wavelength was 227 nm. Results The calibration curve of fluoxetine hydrochloride in plasma was linear in the concentration range of 0.5~50 ng/ml and coefficient was 0.996 9. The intra-assay precision did not exceed 8.06% and inter-assay precision did not exceed 10.46% for low, medium and high quality samples, respectively. The average recovery of the described method was 91.7%,97.06% and 100.75%, respectively. Conclusion In comparison with previous work, the improved chromatographic method was more economical, less polluted and better protected column, besides simple preparation of plasma samples, high reproducibility, and high selectivity.
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