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ZENG Lingjun, CHEN Xu, ZHANG Lingna, ZHANG Jialiang, SONG Hongtao, ZHOU Xin. Preparation of propranolol hydrochloride cubosomes by pH gradient method[J]. Journal of Pharmaceutical Practice and Service, 2021, 39(6): 538-541, 565. doi: 10.12206/j.issn.1006-0111.202103034
Citation:
XU Xue-jun, TAO Qing, XU De-qin. Determination of fluoxetine hydrochloride in plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(3): 222-225. doi: 10.3969/j.issn.1006-0111.2012.03.019
Determination of fluoxetine hydrochloride in plasma by HPLC
Department of Pharmacy, Anhui General Hospital of the Chinese People's Armed Police Forces, Hefei 230061, China
2.
Department of Pharmacy ,The 108th Hospital of PLA, Shanghai 200336,China
Received Date: 2012-02-10
Rev Recd Date:
2012-03-27
Abstract
Objective To establish and optimize a simple, rapid, reliable, high sensitive and high selective method for the determination of fluoxetine hydrochloride in plasma by high-performance liquid chromatographic (HPLC) so as to monitor the clinical drug use. Methods The plasma samples were deproteined by acetonitrile. Separation was performed on a Hypersil ODS2 column(5 μm, 4.6 mm×200 mm). The mobile phase contained acetonitrile-25 mmol/ L phosphate buffer(pH6.5)(34:66). The flow rate and sample volume injected were 1.2 ml/min and 20 μl, respectively. Detection wavelength was 227 nm. Results The calibration curve of fluoxetine hydrochloride in plasma was linear in the concentration range of 0.5~50 ng/ml and coefficient was 0.996 9. The intra-assay precision did not exceed 8.06% and inter-assay precision did not exceed 10.46% for low, medium and high quality samples, respectively. The average recovery of the described method was 91.7%,97.06% and 100.75%, respectively. Conclusion In comparison with previous work, the improved chromatographic method was more economical, less polluted and better protected column, besides simple preparation of plasma samples, high reproducibility, and high selectivity.
Cheer SM, Goa KL. Fluoxetine: a review of its therapeutic potential in the treatment of depression associated with physical illness[J]. Drugs, 2001, 61(1)∶81.
Franceschi L, Faggiani A, Furlanut M. A simple method to monitor serum concentrations of fluoxetine and its major metabolite for pharmacokinetic studies[J]. J Pharm Biomed Anal. 2009, 49(2):554.
Abstract: Objective To establish and optimize a simple, rapid, reliable, high sensitive and high selective method for the determination of fluoxetine hydrochloride in plasma by high-performance liquid chromatographic (HPLC) so as to monitor the clinical drug use. Methods The plasma samples were deproteined by acetonitrile. Separation was performed on a Hypersil ODS2 column(5 μm, 4.6 mm×200 mm). The mobile phase contained acetonitrile-25 mmol/ L phosphate buffer(pH6.5)(34:66). The flow rate and sample volume injected were 1.2 ml/min and 20 μl, respectively. Detection wavelength was 227 nm. Results The calibration curve of fluoxetine hydrochloride in plasma was linear in the concentration range of 0.5~50 ng/ml and coefficient was 0.996 9. The intra-assay precision did not exceed 8.06% and inter-assay precision did not exceed 10.46% for low, medium and high quality samples, respectively. The average recovery of the described method was 91.7%,97.06% and 100.75%, respectively. Conclusion In comparison with previous work, the improved chromatographic method was more economical, less polluted and better protected column, besides simple preparation of plasma samples, high reproducibility, and high selectivity.
ZENG Lingjun, CHEN Xu, ZHANG Lingna, ZHANG Jialiang, SONG Hongtao, ZHOU Xin. Preparation of propranolol hydrochloride cubosomes by pH gradient method[J]. Journal of Pharmaceutical Practice and Service, 2021, 39(6): 538-541, 565. doi: 10.12206/j.issn.1006-0111.202103034
Citation:
XU Xue-jun, TAO Qing, XU De-qin. Determination of fluoxetine hydrochloride in plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(3): 222-225. doi: 10.3969/j.issn.1006-0111.2012.03.019
Cheer SM, Goa KL. Fluoxetine: a review of its therapeutic potential in the treatment of depression associated with physical illness[J]. Drugs, 2001, 61(1)∶81.
Franceschi L, Faggiani A, Furlanut M. A simple method to monitor serum concentrations of fluoxetine and its major metabolite for pharmacokinetic studies[J]. J Pharm Biomed Anal. 2009, 49(2):554.
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