Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 30 Issue 5
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2012  >  30(5): 378-379,389

ZHANG Hong-wu, REN Heng-chun, DING Gang, LI Rong-tao, WEI Jian-he, ZOU Zhong-mei. Determination of two flavones in Polygonum hydropiper Linn[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 378-379,389. doi: 10.3969/j.issn.1006-0111.2012.05.017
Citation: ZHANG Hong-wu, REN Heng-chun, DING Gang, LI Rong-tao, WEI Jian-he, ZOU Zhong-mei. Determination of two flavones in Polygonum hydropiper Linn[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 378-379,389. doi: 10.3969/j.issn.1006-0111.2012.05.017
shu

Determination of two flavones in Polygonum hydropiper Linn

doi: 10.3969/j.issn.1006-0111.2012.05.017
  • 1.

    Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100193, China

  • 2.

    Hainan Branch Institute of Medicinal Plant, Chinese Academy of Medical Science and Peking Union Medical College, Wanning 571533, China

  • Received Date: 2011-11-30
  • Rev Recd Date: 2012-05-17
  • To established the HPLC method for simultaneously determination of quercitin and quercitrin in Polygonum hydropiper Linn. Methods A VertiSep GES C18 (250 mm ×4.6 mm,5.0 μm)column was adopted with a mobile phase of acetonitrile-0.1% formic acid water(19:81) at the flow rate of 1 ml/min and the column temperature was set at 30℃.The detection wavelength was 350 nm. Results The linear range of quercitin and quercitrin was 4.827~80.455 μg／ml(r=0.999 9, n=5) and 2.934~48.900 μg/ml (r=0.999 7, n=5), respectively, and the average recovery(n=9) was 97.98%,98.29%, RSD was 1.91%,1.84% respectively. Conclusions The method was accurate, simple, reproducible, and could be used to determine the contents of quercitin and quercitrin in Polygonum hydropiper Linn.
  • [1] 全国中草药汇编编写组. 全国中草药汇编[M]. 北京:人民卫生出版社, 1986:896.
    [2] Yagi A, Uemura T, Okamura N, et al. Antioxidative sulphated flavonoids in leaves of Polygonum hydropiper[J]. Phytochemistry, 1994, 35 (4):885.
    [3] Haraguchi H, Hashimoto K, Yagi A. Antioxidative substances in leaves of Polygonum hydropiper[J]. J Agric Food Chem, 1992, 40 (8):1349.
    [4] Haraguchi H, Ohmi I, Sakai S, et al. Effect of Polygonum hydropiper flavonoids on lens aldose reductase and related enzymes[J]. J Nat Prod, 1996, 59 (4):443.
    [5] 巩忠福,杨国林,严作廷,等. 蓼属植物的化学成分与药理学活性研究进展[J]. 中草药,2002,33(1):82.
    [6] 罗杰,陆霞. HPLC法测定水辣蓼中芦丁的含量[J]. 中草药,2004,35(3):285.
    [7] 国家中医药管理局《中华本草》编委会.中华本草第6卷[M]. 上海:上海科学技术出版社,1999:662
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(3736) PDF downloads(260) Cited by()

Related
Proportional views

Determination of two flavones in Polygonum hydropiper Linn

doi: 10.3969/j.issn.1006-0111.2012.05.017
  • 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100193, China
  • 2. Hainan Branch Institute of Medicinal Plant, Chinese Academy of Medical Science and Peking Union Medical College, Wanning 571533, China
  • Received Date: 2011-11-30
  • Rev Recd Date: 2012-05-17

Abstract: To established the HPLC method for simultaneously determination of quercitin and quercitrin in Polygonum hydropiper Linn. Methods A VertiSep GES C18 (250 mm ×4.6 mm,5.0 μm)column was adopted with a mobile phase of acetonitrile-0.1% formic acid water(19:81) at the flow rate of 1 ml/min and the column temperature was set at 30℃.The detection wavelength was 350 nm. Results The linear range of quercitin and quercitrin was 4.827~80.455 μg／ml(r=0.999 9, n=5) and 2.934~48.900 μg/ml (r=0.999 7, n=5), respectively, and the average recovery(n=9) was 97.98%,98.29%, RSD was 1.91%,1.84% respectively. Conclusions The method was accurate, simple, reproducible, and could be used to determine the contents of quercitin and quercitrin in Polygonum hydropiper Linn.

ZHANG Hong-wu, REN Heng-chun, DING Gang, LI Rong-tao, WEI Jian-he, ZOU Zhong-mei. Determination of two flavones in Polygonum hydropiper Linn[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 378-379,389. doi: 10.3969/j.issn.1006-0111.2012.05.017
Citation: ZHANG Hong-wu, REN Heng-chun, DING Gang, LI Rong-tao, WEI Jian-he, ZOU Zhong-mei. Determination of two flavones in Polygonum hydropiper Linn[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 378-379,389. doi: 10.3969/j.issn.1006-0111.2012.05.017
shu

    HTML

Reference (7)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return