Determinatin of chlorogenic acid in hawthorn by HPLC

LIANG Rui-shu; ZHANG Chuan; ZOU Hao; JIN Lei

doi:10.3969/j.issn.1006-0111.2012.06.018

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Objective To establish a HPLC method for determination of chlorogenic acid in the hawthorn. Methods Sunfire C₁₈ (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was methanol:sodium dihydrogen phosphate(25:75). The flow rate was 1.0 ml/min, and the temperature was at 28℃. The detection wavelength was 327 nm. Results The calibration curve was at the range of 7.03~70.3 μg/ml (r=0.999 6), RSD was 0. 92% (n=6) and the average recovery was 96.92%. Conclusion The method was simple, reliable, accurate for determination of chlorogenic acid in the hawthorn.

Determinatin of chlorogenic acid in hawthorn by HPLC

1. Guang Dong Shuang Lin Bio-pharmacy Company Limited, Zhanjiang 524009, China

2. New Drug Research Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2012-06-01

Rev Recd Date: 2012-09-12

Key words:

chlorogenic acid /
HPLC /
hawthorn

Abstract: Objective To establish a HPLC method for determination of chlorogenic acid in the hawthorn. Methods Sunfire C₁₈ (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was methanol:sodium dihydrogen phosphate(25:75). The flow rate was 1.0 ml/min, and the temperature was at 28℃. The detection wavelength was 327 nm. Results The calibration curve was at the range of 7.03~70.3 μg/ml (r=0.999 6), RSD was 0. 92% (n=6) and the average recovery was 96.92%. Conclusion The method was simple, reliable, accurate for determination of chlorogenic acid in the hawthorn.

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Reference (9)

[1]	郑虎占, 董泽宏, 佘靖. 中药现代研究与应用:第一卷[M]. 北京:学苑出版社, 1997:597.
[2]	冯凤莲, 张卉朱. 山楂的研究进展[J].河北医科大学学报, 1997, 18(6):383.
[3]	高光跃,冯毓秀,秦秀琴.山楂类果实的化学成分分析及其质量评价[J].药学学报,1995,30(2):138.
[4]	袁华,邓良,孙炎彬,等.可见分光光度法测定杜仲叶中的绿原酸[J].生物加工过程,2007,5(1):71.
[5]	朱玉,张书胜,张西林,等.薄层色谱法分析葵花仁粕中的绿原酸[J].色谱,2001,19(1):82.
[6]	张风云,毛富春.用毛细管区带电泳技术测定杜仲叶中绿原酸含量的研究[J].分析测试学报,1996,15(3):66.
[7]	崔伟伟,范永春.不同提取方法测定野马追药材中绿原酸含量比较研究[J].辽宁中医药大学学报,2011,13(3):178.
[8]	韩哲,崔同, 锁然, 等. 山楂果实中活性成分的高效液相色谱法[J].食品科技, 2006(6):124.
[9]	胡志国,王远兴. 反相高效液相色谱法测定山楂中绿原酸的含量[J].食品科学,2007(11):407.

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Determinatin of chlorogenic acid in hawthorn by HPLC

doi: 10.3969/j.issn.1006-0111.2012.06.018

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