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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(1): 38-41

HE Xiaobo, ZHU Jiahui, GAO Fangyuan. Progress on pharmacokinetics of biotechnology drugs[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 394-399. doi: 10.3969/j.issn.1006-0111.2019.05.003
Citation: ZHOU Xi, TANG Ying, SUN Huan, WANG Qing-min, LEI Cheng-xiang. Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 38-41. doi: 10.3969/j.issn.1006-0111.2013.01.011
shu

Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells

doi: 10.3969/j.issn.1006-0111.2013.01.011
  • 1.

    Hubei University of Traditional Chinese Medicine, Wuhan 430065 China

  • 2.

    Institute of Naval Medicine, Shanghai 200433 China

  • Received Date: 2012-09-26
  • Rev Recd Date: 2012-10-30
  • Objective To investigate the proliferation inhibition of total alkaloids from Pinellia ternate (TATP) in human lung cancer line A549. Methods The proliferation inhibition of human lung cancer cell line A549 treated with TATP was measured with methyl thiazolil tetracolium colorimetic method and plate clone formation assay. The DNA damage of A549 cell induced by TATP was detected with the single cell gel electrophoresis (SCGE). Results The proliferation in A549 cell treated with TATP was decreased significantly compared with control group and the proliferation inhibition was positively correlated to the TATP concentration and the reaction time. The difference between the control group and the TATP experimental groups was significant in statistic. Compared with the control group, the tail DNA content, the tail length and the tail movement of the TATP experimental groups were different significantly and presented a dose-effect relationship. Conclusion TATP could inhibit the proliferation of A549 cells in vitro, which might be correlated to the DNA damage induced by TAPT.
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    [8] 蒋亦燕, 尹丽慧, 费正华, 等. 去甲斑蝥酸钠对耐顺铂肺腺癌A549/DDP细胞耐药相关基因表达的影响[J].中药药理与临床, 2009, 25(5): 37.
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    [10] 冯觉平, 孔庆志, 黄 涛, 等. 三氧化二砷逆转人肺腺A549/R细胞耐药及对GSTs表达的影响[J]. 肿瘤防治研究, 2005, 32(8): 493.
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    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 38.0 %其他: 38.0 %其他: 0.3 %其他: 0.3 %China: 0.8 %China: 0.8 %[]: 0.1 %[]: 0.1 %上海: 2.6 %上海: 2.6 %东京: 0.6 %东京: 0.6 %东莞: 0.8 %东莞: 0.8 %临汾: 0.1 %临汾: 0.1 %保定: 0.3 %保定: 0.3 %北京: 6.6 %北京: 6.6 %十堰: 0.1 %十堰: 0.1 %南京: 1.2 %南京: 1.2 %南昌: 0.7 %南昌: 0.7 %合肥: 0.6 %合肥: 0.6 %呼伦贝尔: 0.3 %呼伦贝尔: 0.3 %哈尔滨: 0.1 %哈尔滨: 0.1 %天津: 1.0 %天津: 1.0 %广州: 1.4 %广州: 1.4 %延边: 0.1 %延边: 0.1 %张家口: 0.3 %张家口: 0.3 %德罕: 0.3 %德罕: 0.3 %成都: 0.3 %成都: 0.3 %扬州: 0.1 %扬州: 0.1 %昆明: 0.1 %昆明: 0.1 %杭州: 1.2 %杭州: 1.2 %武汉: 1.2 %武汉: 1.2 %沈阳: 0.6 %沈阳: 0.6 %洛阳: 0.1 %洛阳: 0.1 %济南: 0.1 %济南: 0.1 %石家庄: 0.1 %石家庄: 0.1 %秦皇岛: 0.1 %秦皇岛: 0.1 %芒廷维尤: 15.6 %芒廷维尤: 15.6 %芝加哥: 0.3 %芝加哥: 0.3 %苏州: 0.4 %苏州: 0.4 %衡水: 0.1 %衡水: 0.1 %西宁: 15.0 %西宁: 15.0 %西安: 0.3 %西安: 0.3 %许昌: 0.1 %许昌: 0.1 %赣州: 0.3 %赣州: 0.3 %赤峰: 0.4 %赤峰: 0.4 %达尔斯: 0.1 %达尔斯: 0.1 %运城: 0.7 %运城: 0.7 %郑州: 0.6 %郑州: 0.6 %重庆: 0.3 %重庆: 0.3 %长沙: 3.2 %长沙: 3.2 %长治: 0.3 %长治: 0.3 %青岛: 0.7 %青岛: 0.7 %黄冈: 1.1 %黄冈: 1.1 %黄冈市麻城: 0.1 %黄冈市麻城: 0.1 %其他其他China[]上海东京东莞临汾保定北京十堰南京南昌合肥呼伦贝尔哈尔滨天津广州延边张家口德罕成都扬州昆明杭州武汉沈阳洛阳济南石家庄秦皇岛芒廷维尤芝加哥苏州衡水西宁西安许昌赣州赤峰达尔斯运城郑州重庆长沙长治青岛黄冈黄冈市麻城Highcharts.com

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Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells

doi: 10.3969/j.issn.1006-0111.2013.01.011
  • 1. Hubei University of Traditional Chinese Medicine, Wuhan 430065 China
  • 2. Institute of Naval Medicine, Shanghai 200433 China
  • Received Date: 2012-09-26
  • Rev Recd Date: 2012-10-30

Abstract: Objective To investigate the proliferation inhibition of total alkaloids from Pinellia ternate (TATP) in human lung cancer line A549. Methods The proliferation inhibition of human lung cancer cell line A549 treated with TATP was measured with methyl thiazolil tetracolium colorimetic method and plate clone formation assay. The DNA damage of A549 cell induced by TATP was detected with the single cell gel electrophoresis (SCGE). Results The proliferation in A549 cell treated with TATP was decreased significantly compared with control group and the proliferation inhibition was positively correlated to the TATP concentration and the reaction time. The difference between the control group and the TATP experimental groups was significant in statistic. Compared with the control group, the tail DNA content, the tail length and the tail movement of the TATP experimental groups were different significantly and presented a dose-effect relationship. Conclusion TATP could inhibit the proliferation of A549 cells in vitro, which might be correlated to the DNA damage induced by TAPT.

HE Xiaobo, ZHU Jiahui, GAO Fangyuan. Progress on pharmacokinetics of biotechnology drugs[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 394-399. doi: 10.3969/j.issn.1006-0111.2019.05.003
Citation: ZHOU Xi, TANG Ying, SUN Huan, WANG Qing-min, LEI Cheng-xiang. Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 38-41. doi: 10.3969/j.issn.1006-0111.2013.01.011
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