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Volume 31 Issue 1
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(1): 38-41

ZHOU Xi, TANG Ying, SUN Huan, WANG Qing-min, LEI Cheng-xiang. Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 38-41. doi: 10.3969/j.issn.1006-0111.2013.01.011
Citation: ZHOU Xi, TANG Ying, SUN Huan, WANG Qing-min, LEI Cheng-xiang. Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 38-41. doi: 10.3969/j.issn.1006-0111.2013.01.011
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Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells

doi: 10.3969/j.issn.1006-0111.2013.01.011
  • 1.

    Hubei University of Traditional Chinese Medicine, Wuhan 430065 China

  • 2.

    Institute of Naval Medicine, Shanghai 200433 China

  • Received Date: 2012-09-26
  • Rev Recd Date: 2012-10-30
  • Objective To investigate the proliferation inhibition of total alkaloids from Pinellia ternate (TATP) in human lung cancer line A549. Methods The proliferation inhibition of human lung cancer cell line A549 treated with TATP was measured with methyl thiazolil tetracolium colorimetic method and plate clone formation assay. The DNA damage of A549 cell induced by TATP was detected with the single cell gel electrophoresis (SCGE). Results The proliferation in A549 cell treated with TATP was decreased significantly compared with control group and the proliferation inhibition was positively correlated to the TATP concentration and the reaction time. The difference between the control group and the TATP experimental groups was significant in statistic. Compared with the control group, the tail DNA content, the tail length and the tail movement of the TATP experimental groups were different significantly and presented a dose-effect relationship. Conclusion TATP could inhibit the proliferation of A549 cells in vitro, which might be correlated to the DNA damage induced by TAPT.
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    [8] 蒋亦燕, 尹丽慧, 费正华, 等. 去甲斑蝥酸钠对耐顺铂肺腺癌A549/DDP细胞耐药相关基因表达的影响[J].中药药理与临床, 2009, 25(5): 37.
    [9] 梁 莉, 刘叙仪, 王 洁, 等. 马蔺子素对耐顺铂人肺腺癌细胞系A549/DDP耐药逆转及可能的机制[J].中华医学杂志, 2001, 81(22): 1392.
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    [11] 张梅春, 赵子文, 曾 军, 等. 姜黄素对耐顺铂人肺腺癌细胞Survivin表达及其化疗敏感性的影响[J]. 中华肿瘤防治杂志, 2007, 14(19): 1454.
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Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells

doi: 10.3969/j.issn.1006-0111.2013.01.011
  • 1. Hubei University of Traditional Chinese Medicine, Wuhan 430065 China
  • 2. Institute of Naval Medicine, Shanghai 200433 China
  • Received Date: 2012-09-26
  • Rev Recd Date: 2012-10-30

Abstract: Objective To investigate the proliferation inhibition of total alkaloids from Pinellia ternate (TATP) in human lung cancer line A549. Methods The proliferation inhibition of human lung cancer cell line A549 treated with TATP was measured with methyl thiazolil tetracolium colorimetic method and plate clone formation assay. The DNA damage of A549 cell induced by TATP was detected with the single cell gel electrophoresis (SCGE). Results The proliferation in A549 cell treated with TATP was decreased significantly compared with control group and the proliferation inhibition was positively correlated to the TATP concentration and the reaction time. The difference between the control group and the TATP experimental groups was significant in statistic. Compared with the control group, the tail DNA content, the tail length and the tail movement of the TATP experimental groups were different significantly and presented a dose-effect relationship. Conclusion TATP could inhibit the proliferation of A549 cells in vitro, which might be correlated to the DNA damage induced by TAPT.

ZHOU Xi, TANG Ying, SUN Huan, WANG Qing-min, LEI Cheng-xiang. Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 38-41. doi: 10.3969/j.issn.1006-0111.2013.01.011
Citation: ZHOU Xi, TANG Ying, SUN Huan, WANG Qing-min, LEI Cheng-xiang. Proliferation inhibition of total alkaloids from Pinellia Ternata in human lung cancer cells[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 38-41. doi: 10.3969/j.issn.1006-0111.2013.01.011
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