SHA Ri-na, HE Chun-long, WANG Huan-yun. Determination of hydroxysaffor yellow A in Yihe decoction by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 140-142. doi: 10.3969/j.issn.1006-0111.2013.02.017
| Citation:
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SHA Ri-na, HE Chun-long, WANG Huan-yun. Determination of hydroxysaffor yellow A in Yihe decoction by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 140-142. doi: 10.3969/j.issn.1006-0111.2013.02.017
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Determination of hydroxysaffor yellow A in Yihe decoction by HPLC
- 1.
Inner Mongolia Medical university, Department of pharmacy, Second affiliated hospita
- 2.
School of Pharmay, Hohhot 010030, China
- Received Date: 2012-06-25
- Rev Recd Date:
2012-09-19
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Abstract
Objective To establish a RP-HPLC method for the determination of hydroxysaffor yellows A in Yihe decoction. Methods The separation was performed on a Yilite C18 column(250 mm×4.6 mm, 5 μm). The mobile phase was methanol-0.5% acetic acid(20:80) with a flow rate of 1.0 ml/min. The detection wavelength was set at 403 nm. Results Hydroxysaffor yellow A in the range of 0.816 0~32.64 μg/ml showed a good linear relationship (r=0.999 9). The average recovery was 101.0% and RSD was 1.8% (n=6). Conclusion This accurate and reliable HPLC method could be used for the determination of hydroxysaffor yellow A in Yihe decoction.
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