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Volume 31 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(4): 273-276

YANG Ming-hua, SUN Xue-mei, CHEN Gui-liang, ZHANG Yu-min. Qualitative and quantitative analysis of metronidazole injection solutions by confocal microprobe Raman spectroscopy[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 273-276. doi: 10.3969/j.issn.1006-0111.2013.04.009
Citation: YANG Ming-hua, SUN Xue-mei, CHEN Gui-liang, ZHANG Yu-min. Qualitative and quantitative analysis of metronidazole injection solutions by confocal microprobe Raman spectroscopy[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 273-276. doi: 10.3969/j.issn.1006-0111.2013.04.009
shu

Qualitative and quantitative analysis of metronidazole injection solutions by confocal microprobe Raman spectroscopy

doi: 10.3969/j.issn.1006-0111.2013.04.009
  • 1.

    Shanghai Pudong Institute for Food and Drug Control, Shanghai 201203, China

  • 2.

    Shanghai Institute for Food and Drug Control, Shanghai 201203, China

  • 3.

    Shanghai Municipal Food and Drug Administration, Shanghai 200010, China

  • Received Date: 2012-07-04
  • Rev Recd Date: 2012-10-29
  • Objective To explore the application of Raman spectroscopy in metronidazole injection solution analysis. Methods The variation of metronidazole (MET) injections' Raman spectra was analyzed when the pH varied from 1 to 12, and the related substances (2-methyl-5-nitroimidazole) in MET injection were detected. Two quantitative analysis techniques, multivariate partial least squares (PLS) and principal component regression (PCR) were compared in MET sample quantification. The quantification results of commercial MET injection product were compared with pharmacopoeias suggested UV Spectrometry method. Results The pH differences were reflected in Raman spectra. Because of the low concentration of the related substance in Commercial MET injection (1 mg/L), the related substance could not be detected by Raman spectrum. Conclusion Both PLS and PCR could reach satisfactory prediction, and the concentrations found from Raman and UV spectrometry showed no significant differences according to t-test (α=0.05).
  • [1] Ruchita SD, Agrawal YK. Raman spectroscopy:recent advancements, techniques and applications[J]. Vib Spectrosc,2011,8:3.
    [2] Sylwester M, Roman S. Quantitative determination of diclofenac sodium and aminophylline in injection solutions by FT-Raman spectroscopy[J]. J Pharma & Bio Analy,2006,40:1235.
    [3] Orkoula MG, Kontoyannis CG,Markopoulou CK. Validation of a direct non-destructive quantitative analysis of amiodarone hydrochloride in Angoron formulations using FT-Raman spectroscopy[J]. Talanta,2007,73:258.
    [4] 许莉莉,丁库克,郭学谦,等.水杨酸、对甲氧基苯甲酸和甲硝唑的拉曼光谱[J].首都医科大学学报,2006,27(6):785..
    [5] 杨玉平,陈 笑,姚 飞,等.甲硝唑、替硝唑和奥硝唑的拉曼光谱研究[J].中央民族大学学报(自然科学版),2010,19(4):26.
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Qualitative and quantitative analysis of metronidazole injection solutions by confocal microprobe Raman spectroscopy

doi: 10.3969/j.issn.1006-0111.2013.04.009
  • 1. Shanghai Pudong Institute for Food and Drug Control, Shanghai 201203, China
  • 2. Shanghai Institute for Food and Drug Control, Shanghai 201203, China
  • 3. Shanghai Municipal Food and Drug Administration, Shanghai 200010, China
  • Received Date: 2012-07-04
  • Rev Recd Date: 2012-10-29

Abstract: Objective To explore the application of Raman spectroscopy in metronidazole injection solution analysis. Methods The variation of metronidazole (MET) injections' Raman spectra was analyzed when the pH varied from 1 to 12, and the related substances (2-methyl-5-nitroimidazole) in MET injection were detected. Two quantitative analysis techniques, multivariate partial least squares (PLS) and principal component regression (PCR) were compared in MET sample quantification. The quantification results of commercial MET injection product were compared with pharmacopoeias suggested UV Spectrometry method. Results The pH differences were reflected in Raman spectra. Because of the low concentration of the related substance in Commercial MET injection (1 mg/L), the related substance could not be detected by Raman spectrum. Conclusion Both PLS and PCR could reach satisfactory prediction, and the concentrations found from Raman and UV spectrometry showed no significant differences according to t-test (α=0.05).

YANG Ming-hua, SUN Xue-mei, CHEN Gui-liang, ZHANG Yu-min. Qualitative and quantitative analysis of metronidazole injection solutions by confocal microprobe Raman spectroscopy[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 273-276. doi: 10.3969/j.issn.1006-0111.2013.04.009
Citation: YANG Ming-hua, SUN Xue-mei, CHEN Gui-liang, ZHANG Yu-min. Qualitative and quantitative analysis of metronidazole injection solutions by confocal microprobe Raman spectroscopy[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 273-276. doi: 10.3969/j.issn.1006-0111.2013.04.009
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