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Volume 31 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(4): 302-303,309

WANG Yan-an, WANG Xiao-bo, Liu dan, XI Rong-gang. Determination of contents of ginsenoside Rg1, Re and Rb1 in Naili capsule Nov. 3 by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 302-303,309. doi: 10.3969/j.issn.1006-0111.2013.04.018
Citation: WANG Yan-an, WANG Xiao-bo, Liu dan, XI Rong-gang. Determination of contents of ginsenoside Rg1, Re and Rb1 in Naili capsule Nov. 3 by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 302-303,309. doi: 10.3969/j.issn.1006-0111.2013.04.018
shu

Determination of contents of ginsenoside Rg1, Re and Rb1 in Naili capsule Nov. 3 by HPLC

doi: 10.3969/j.issn.1006-0111.2013.04.018
  • 1.

    Liaoning University of Traditional Chinese Medicine, Dalian 116600, China

  • 2.

    The 210th Hospital of PLA, Dalian 116015, China

  • Received Date: 2013-03-14
  • Rev Recd Date: 2013-04-15
  • Objective To establish a method for the determination of ginsenoside Rg1、ginsenoside Re、ginsenoside Rb1 in Naili capsule Nov. 3. Methods HPLC with C18 column was revised to control the content of Radix Panax quinguefolium, the detective wavelength was set at 203 nm, reflux extraction with water saturation of n-butyl alcohol, true and false identification by TCL, the mobile phase was a linear gradient of acetonitrile(A) and 0.1% phosphate acid(B). Results Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 showed the good linear relationships at the range of 0.2~1.0 μg (r=0.999 2), 0.8~4.0 μg (r=0.999 1), 2.0~10.0μg (r=0.999 1), respectively. Their recovery rates:Ginsenoside Rg1 average recovery was 96.67%, RSD=1.14%;Ginsenoside Re average recovery was 100.52%, RSD=1.18%;and Ginsenoside Rb1 average recovery was 96.94%, RSD=0.59%, respectively. Conclusion These methods were simple and accurate, and was repeatable, which could be used in the quality control of Naili capsule Nov. 3.
  • [1] 中国药典2010版.一部[S]. 2010:122.
    [2] 刘莉,程龙琼,周世玉.西洋参中人参皂苷Rg1人参皂苷Re和人参皂苷Rb1的含量测定[J].医药导报,2011,30(9):1208.
    [3] 汪冰,尤慧莲,徐丽华.HPLC法测定芪参胶囊中的五种化学成分[J].中成药,2012,34(3):483.
    [4] 范辉,顾泉琳,贝伟剑.HPLC法测定乌参醒脑滴丸中人参皂苷Rg1、Re、Rb1和Rd[J].中成药,2012,134(7):1280.
    [5] 王贵金,贾继明,刘兴国,等.高效液相色谱法测定注射用萎肌灵中人参皂苷Rg1、Re、Rb1的含量[J].中国医院药学杂志,2011,3(15):375.
    [6] 王旭,邬国庆,张小茜.HPLC法测定进口西洋参中人参皂苷Rb1、Re、Rg1的含量[J].中国实验方剂学杂志,2004,10(6)27.
    [7] 于敏,焦连庆,严仲恺,等.西洋参饮片的高效液相特征图谱[J].中国医院药学杂志,2011,31(15):1310.
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Determination of contents of ginsenoside Rg1, Re and Rb1 in Naili capsule Nov. 3 by HPLC

doi: 10.3969/j.issn.1006-0111.2013.04.018
  • 1. Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
  • 2. The 210th Hospital of PLA, Dalian 116015, China
  • Received Date: 2013-03-14
  • Rev Recd Date: 2013-04-15

Abstract: Objective To establish a method for the determination of ginsenoside Rg1、ginsenoside Re、ginsenoside Rb1 in Naili capsule Nov. 3. Methods HPLC with C18 column was revised to control the content of Radix Panax quinguefolium, the detective wavelength was set at 203 nm, reflux extraction with water saturation of n-butyl alcohol, true and false identification by TCL, the mobile phase was a linear gradient of acetonitrile(A) and 0.1% phosphate acid(B). Results Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 showed the good linear relationships at the range of 0.2~1.0 μg (r=0.999 2), 0.8~4.0 μg (r=0.999 1), 2.0~10.0μg (r=0.999 1), respectively. Their recovery rates:Ginsenoside Rg1 average recovery was 96.67%, RSD=1.14%;Ginsenoside Re average recovery was 100.52%, RSD=1.18%;and Ginsenoside Rb1 average recovery was 96.94%, RSD=0.59%, respectively. Conclusion These methods were simple and accurate, and was repeatable, which could be used in the quality control of Naili capsule Nov. 3.

WANG Yan-an, WANG Xiao-bo, Liu dan, XI Rong-gang. Determination of contents of ginsenoside Rg1, Re and Rb1 in Naili capsule Nov. 3 by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 302-303,309. doi: 10.3969/j.issn.1006-0111.2013.04.018
Citation: WANG Yan-an, WANG Xiao-bo, Liu dan, XI Rong-gang. Determination of contents of ginsenoside Rg1, Re and Rb1 in Naili capsule Nov. 3 by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 302-303,309. doi: 10.3969/j.issn.1006-0111.2013.04.018
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