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XU Yi, HE Ruihua, HUANG Jin. Study on the treatment of cerebral infarction with Chinese medicine Shenmajingfu granule by network pharmacology[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(2): 66-72. doi: 10.12206/j.issn.2097-2024.202303013
Citation:
WANG Yan-an, WANG Xiao-bo, Liu dan, XI Rong-gang. Determination of contents of ginsenoside Rg1, Re and Rb1 in Naili capsule Nov. 3 by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 302-303,309. doi: 10.3969/j.issn.1006-0111.2013.04.018
Determination of contents of ginsenoside Rg1, Re and Rb1 in Naili capsule Nov. 3 by HPLC
Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
2.
The 210th Hospital of PLA, Dalian 116015, China
Received Date: 2013-03-14
Rev Recd Date:
2013-04-15
Abstract
Objective To establish a method for the determination of ginsenoside Rg1、ginsenoside Re、ginsenoside Rb1 in Naili capsule Nov. 3. Methods HPLC with C18 column was revised to control the content of Radix Panax quinguefolium, the detective wavelength was set at 203 nm, reflux extraction with water saturation of n-butyl alcohol, true and false identification by TCL, the mobile phase was a linear gradient of acetonitrile(A) and 0.1% phosphate acid(B). Results Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 showed the good linear relationships at the range of 0.2~1.0 μg (r=0.999 2), 0.8~4.0 μg (r=0.999 1), 2.0~10.0μg (r=0.999 1), respectively. Their recovery rates:Ginsenoside Rg1 average recovery was 96.67%, RSD=1.14%;Ginsenoside Re average recovery was 100.52%, RSD=1.18%;and Ginsenoside Rb1 average recovery was 96.94%, RSD=0.59%, respectively. Conclusion These methods were simple and accurate, and was repeatable, which could be used in the quality control of Naili capsule Nov. 3.
Abstract: Objective To establish a method for the determination of ginsenoside Rg1、ginsenoside Re、ginsenoside Rb1 in Naili capsule Nov. 3. Methods HPLC with C18 column was revised to control the content of Radix Panax quinguefolium, the detective wavelength was set at 203 nm, reflux extraction with water saturation of n-butyl alcohol, true and false identification by TCL, the mobile phase was a linear gradient of acetonitrile(A) and 0.1% phosphate acid(B). Results Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 showed the good linear relationships at the range of 0.2~1.0 μg (r=0.999 2), 0.8~4.0 μg (r=0.999 1), 2.0~10.0μg (r=0.999 1), respectively. Their recovery rates:Ginsenoside Rg1 average recovery was 96.67%, RSD=1.14%;Ginsenoside Re average recovery was 100.52%, RSD=1.18%;and Ginsenoside Rb1 average recovery was 96.94%, RSD=0.59%, respectively. Conclusion These methods were simple and accurate, and was repeatable, which could be used in the quality control of Naili capsule Nov. 3.
XU Yi, HE Ruihua, HUANG Jin. Study on the treatment of cerebral infarction with Chinese medicine Shenmajingfu granule by network pharmacology[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(2): 66-72. doi: 10.12206/j.issn.2097-2024.202303013
Citation:
WANG Yan-an, WANG Xiao-bo, Liu dan, XI Rong-gang. Determination of contents of ginsenoside Rg1, Re and Rb1 in Naili capsule Nov. 3 by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 302-303,309. doi: 10.3969/j.issn.1006-0111.2013.04.018
XU Yi, HE Ruihua, HUANG Jin. Study on the treatment of cerebral infarction with Chinese medicine Shenmajingfu granule by network pharmacology[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(2): 66-72. doi: 10.12206/j.issn.2097-2024.202303013
XU Yi, HE Ruihua, HUANG Jin. Study on the treatment of cerebral infarction with Chinese medicine Shenmajingfu granule by network pharmacology[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(2): 66-72. doi: 10.12206/j.issn.2097-2024.202303013