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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(5): 384-385,392

XIN Bo, WAN Lili, WANG Jing, LIU Jinbian, HUO Yan, GUO Cheng. Cisatracurium assay in human plasma by LC-MS[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(2): 148-151. doi: 10.3969/j.issn.1006-0111.201909001
Citation: CAO Jin-hua, GU Li, LV Lei, LI Yue-yue, ZHOU Gui-chen, ZHAO Liang, ZHANG Guo-qing. Determination of asiaticoside in Centella asiatica and its formula particles by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 384-385,392. doi: 10.3969/j.issn.1006-0111.2013.05.019
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Determination of asiaticoside in Centella asiatica and its formula particles by HPLC-ELSD

doi: 10.3969/j.issn.1006-0111.2013.05.019
  • 1.

    Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China

  • 2.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2012-12-18
  • Rev Recd Date: 2013-04-07
  • Objective To develop a method for determination of assiticoside in Centella asiatica and its formula particles. Methods Hypersil ODS C18 column was used(4.6 mmá250 mm,5 μm),withacetonitrile-water(v/v=3:8) as mobile phase, and a flow rate of 0.6 ml/min. Results The result showed that the calibration curve was linear in the range of 23.2~580.0 μg/ml for asiaticoside, intra-day and inter-day precision results were 2.1% and 3.6%, the average recovery of asiaticoside were 95.2%(RSD=3.2%, n=6) in centella asiatica and 104.6%(RSD=1.0%,n=6) in its formula particles. The contents of asiaticoside in 5 medicinal materials were 0.33%,0.52%,0.22%,0.28% and 0.39%;while in 5 formula particles were 0.40%,0.44%,0.39%,0.44% and 0.42%. Conclusions The method was simple and accurate for quality control of Centella asiatica and its formula particles.
  • [1] 中国药典2010年版一部[S]. 2010:199.
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    [4] 张 莉, 崔晓立. 薄层扫描法测定积雪甙片中积雪草苷的含量[J]. 中国卫生工程学, 2009, 8(3): 174.[JP3]
    [5] Diallo B, Vanhaelen FR, Vanhaelen M. Direct coupling of high-speed counter-current chromatography to thin-layer chromatography:Application to the separation of asiaticoside and madecassoside from Centella asiatica[J]. J Chromatogr A, 1991,558(2): 446.
    [6] 白斌艳,张爱荣,朱小芝,等. 高效液相色谱法测定积雪苷片中积雪草苷和羟基积雪草苷的含量[J]. 山西中医学院学报, 2009, 10(3): 28.[JP2]
    [7] 黄 晟,谷 莉,娄子洋,等. 高效液相法测定积雪草配方颗粒中积雪草苷的含量[J]. 药物分析杂志, 2004, 24(5): 516.
    [8] 向 前,杜书良,唐 强,等. HPLC-ELSD法测定积雪草中积雪草苷的含量[J]. 成都中医药大学学报, 2007, 30(1): 54.
    [9] 于泉林,高文远,陈海霞,等. HPLC-ELSD测定积雪草提取物中积雪草苷的含量[J]. 中国中药杂志, 2007, 32(6): 503.
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    1. 徐佳明，周晓芳，郭建荣. 基于UPLC-MS/MS技术监测老年患者术后血浆中苯磺酸顺阿曲库铵残留. 药学实践与服务. 2023(03): 192-196 . 本站查看

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Determination of asiaticoside in Centella asiatica and its formula particles by HPLC-ELSD

doi: 10.3969/j.issn.1006-0111.2013.05.019
  • 1. Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
  • 2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2012-12-18
  • Rev Recd Date: 2013-04-07

Abstract: Objective To develop a method for determination of assiticoside in Centella asiatica and its formula particles. Methods Hypersil ODS C18 column was used(4.6 mmá250 mm,5 μm),withacetonitrile-water(v/v=3:8) as mobile phase, and a flow rate of 0.6 ml/min. Results The result showed that the calibration curve was linear in the range of 23.2~580.0 μg/ml for asiaticoside, intra-day and inter-day precision results were 2.1% and 3.6%, the average recovery of asiaticoside were 95.2%(RSD=3.2%, n=6) in centella asiatica and 104.6%(RSD=1.0%,n=6) in its formula particles. The contents of asiaticoside in 5 medicinal materials were 0.33%,0.52%,0.22%,0.28% and 0.39%;while in 5 formula particles were 0.40%,0.44%,0.39%,0.44% and 0.42%. Conclusions The method was simple and accurate for quality control of Centella asiatica and its formula particles.

XIN Bo, WAN Lili, WANG Jing, LIU Jinbian, HUO Yan, GUO Cheng. Cisatracurium assay in human plasma by LC-MS[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(2): 148-151. doi: 10.3969/j.issn.1006-0111.201909001
Citation: CAO Jin-hua, GU Li, LV Lei, LI Yue-yue, ZHOU Gui-chen, ZHAO Liang, ZHANG Guo-qing. Determination of asiaticoside in Centella asiatica and its formula particles by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 384-385,392. doi: 10.3969/j.issn.1006-0111.2013.05.019
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