HUANG Yun, ZHANG Zhengyin, JIN Ying, ZHENG YiJing, LI Tiejun, SUN Lili. Analysis of resistance situation and resistance genes of clinical isolates of carbapenem-resistant Klebsiella pneumoniae and Escherichia coli[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(10): 439-444. doi: 10.12206/j.issn.2097-2024.202309059
| Citation:
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CAO Jin-hua, GU Li, LV Lei, LI Yue-yue, ZHOU Gui-chen, ZHAO Liang, ZHANG Guo-qing. Determination of asiaticoside in Centella asiatica and its formula particles by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 384-385,392. doi: 10.3969/j.issn.1006-0111.2013.05.019
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Determination of asiaticoside in Centella asiatica and its formula particles by HPLC-ELSD
- 1.
Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
- 2.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- Received Date: 2012-12-18
- Rev Recd Date:
2013-04-07
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Abstract
Objective To develop a method for determination of assiticoside in Centella asiatica and its formula particles. Methods Hypersil ODS C18 column was used(4.6 mmá250 mm,5 μm),withacetonitrile-water(v/v=3:8) as mobile phase, and a flow rate of 0.6 ml/min. Results The result showed that the calibration curve was linear in the range of 23.2~580.0 μg/ml for asiaticoside, intra-day and inter-day precision results were 2.1% and 3.6%, the average recovery of asiaticoside were 95.2%(RSD=3.2%, n=6) in centella asiatica and 104.6%(RSD=1.0%,n=6) in its formula particles. The contents of asiaticoside in 5 medicinal materials were 0.33%,0.52%,0.22%,0.28% and 0.39%;while in 5 formula particles were 0.40%,0.44%,0.39%,0.44% and 0.42%. Conclusions The method was simple and accurate for quality control of Centella asiatica and its formula particles.
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