ZHAO Wenjun, LIN Yang, LI Pengfei, LIU Yang. Analysis of chemical constituents of Moutan cortex by HPLC-QTOFMS[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(4): 261-265. doi: 10.3969/j.issn.1006-0111.2014.04.006
| Citation:
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ZHAO Wenjun, LIN Yang, LI Pengfei, LIU Yang. Analysis of chemical constituents of Moutan cortex by HPLC-QTOFMS[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(4): 261-265. doi: 10.3969/j.issn.1006-0111.2014.04.006
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Analysis of chemical constituents of Moutan cortex by HPLC-QTOFMS
- 1.
Institute for Drug and Instrument Control, Shenyang Military Region, Shenyang 110026, China
- 2.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- Received Date: 2013-07-05
- Rev Recd Date:
2014-05-14
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Abstract
Objective To analyze the main chemical constituents of Moutan cortex by high performance liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-QTOFMS). Methods A CAPCELL PAK C18(3.0 mm×100 mm, 3 μm) column was used to do chromatographic separation.0.1% aqueous formic acid solution and acetonitrile were used as mobile phase composition.Gradient elution was used with a flow rate of 0.5 ml/min; QTOFMS was applied for qualitative analysis under positive and negative ion mode. Results 48 major constituents of Moutan cortex were identified by QTOFMS and structure-relevant fragment ions under the optimized condition. Conclusion A simple and reliable method with the usage of HPLC-QTOFMS was established to identify the main chemical constituents of Moutan cortex.
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