Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay

DENG Jingjing; JI Shengjun; CHEN Ye; LIN Xiaolin; WANG Rui; XU Lifeng

doi:10.3969/j.issn.1006-0111.2014.04.007

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Objective To evaluated the inhibitory effect of garcinia glycosides on growth of 8 kinds of human tumor cells in vivo by hollow fiber assay and confirm the reliability of hollow fiber assay in anticancer effect by the nude mice xenograft test. Methods Hollow fibers containing tumor cells were inserted underneath the skin of the NOD/SCID mice.The fibers were collected from the mice on the day after the administration and subjected to the stable endpoint MTT assay.The tumor cells of HL-60 and B16 were subcutaneously implanted into the right flank of BALb /c nude mice.The positive control group was treated with cyclophosphamide.Each group was administered for 10 days.24 hours after the last administration, the mice were sacrificed and the tumors were excised and weighted, the inhibition rate of tumor growth was calculated. Results The high-dose group of 8 mg/(kg·d), middle dose group of 4 mg/(kg·d) of garcinia glycosides were measured by hollow fiber assay and nude mice test significantly inhibited the in vivo growth of HL-60 and B16 comparing with those in the solvent control group(P<0.01). Conclusion As a new model by hollow fiber assay to evaluate the inhibitory effect of garcinia glycosides, the test results were basically the same with nude mice test results.It made the experiment more rapidly, accurately and economically.An instruction and reliable evidence for follow-up study of garcinia glycosides was provided in this study.

Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay

School of Pharmacy, Liaoning University, Shenyang 110036, China

Received Date: 2013-03-22

Rev Recd Date: 2013-09-14

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Abstract: Objective To evaluated the inhibitory effect of garcinia glycosides on growth of 8 kinds of human tumor cells in vivo by hollow fiber assay and confirm the reliability of hollow fiber assay in anticancer effect by the nude mice xenograft test. Methods Hollow fibers containing tumor cells were inserted underneath the skin of the NOD/SCID mice.The fibers were collected from the mice on the day after the administration and subjected to the stable endpoint MTT assay.The tumor cells of HL-60 and B16 were subcutaneously implanted into the right flank of BALb /c nude mice.The positive control group was treated with cyclophosphamide.Each group was administered for 10 days.24 hours after the last administration, the mice were sacrificed and the tumors were excised and weighted, the inhibition rate of tumor growth was calculated. Results The high-dose group of 8 mg/(kg·d), middle dose group of 4 mg/(kg·d) of garcinia glycosides were measured by hollow fiber assay and nude mice test significantly inhibited the in vivo growth of HL-60 and B16 comparing with those in the solvent control group(P<0.01). Conclusion As a new model by hollow fiber assay to evaluate the inhibitory effect of garcinia glycosides, the test results were basically the same with nude mice test results.It made the experiment more rapidly, accurately and economically.An instruction and reliable evidence for follow-up study of garcinia glycosides was provided in this study.

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Reference (8)

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Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay

doi: 10.3969/j.issn.1006-0111.2014.04.007

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