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HUANG Fengai, ZHANG Junping. Research progress on active ingredients from traditional Chinese medicine as inhibitors of PD-1/PD-L1 of cancer immune checkpoint[J]. Journal of Pharmaceutical Practice and Service, 2023, 41(5): 277-283, 290. doi: 10.12206/j.issn.2097-2024.202208079
Citation:
DENG Jingjing, JI Shengjun, CHEN Ye, LIN Xiaolin, WANG Rui, XU Lifeng. Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(4): 266-269. doi: 10.3969/j.issn.1006-0111.2014.04.007
Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay
School of Pharmacy, Liaoning University, Shenyang 110036, China
Received Date: 2013-03-22
Rev Recd Date:
2013-09-14
Abstract
Objective To evaluated the inhibitory effect of garcinia glycosides on growth of 8 kinds of human tumor cells in vivo by hollow fiber assay and confirm the reliability of hollow fiber assay in anticancer effect by the nude mice xenograft test. Methods Hollow fibers containing tumor cells were inserted underneath the skin of the NOD/SCID mice.The fibers were collected from the mice on the day after the administration and subjected to the stable endpoint MTT assay.The tumor cells of HL-60 and B16 were subcutaneously implanted into the right flank of BALb /c nude mice.The positive control group was treated with cyclophosphamide.Each group was administered for 10 days.24 hours after the last administration, the mice were sacrificed and the tumors were excised and weighted, the inhibition rate of tumor growth was calculated. Results The high-dose group of 8 mg/(kg·d), middle dose group of 4 mg/(kg·d) of garcinia glycosides were measured by hollow fiber assay and nude mice test significantly inhibited the in vivo growth of HL-60 and B16 comparing with those in the solvent control group(P<0.01). Conclusion As a new model by hollow fiber assay to evaluate the inhibitory effect of garcinia glycosides, the test results were basically the same with nude mice test results.It made the experiment more rapidly, accurately and economically.An instruction and reliable evidence for follow-up study of garcinia glycosides was provided in this study.
Hollingshead MG,Alley MC, Camalier RF,et al.In vivo cultivation of tumor cells in hollow fibers[J].Life Sci,1995,57(2):131-141.
[3]
Lee KH,Rhee KH.Correlative effect between in vivo hollow fiber assay and xenografts assay in drug screening[J].Cancer Res Treat,2005,37(3):196-200.
[4]
Zhang GJ,Chen TB,Bednar B,et al.Optical imaging of tumor cells in hollow fibers:evaluation of the antitumor activities of anticancer drugs and target validation[J].Neoplasia,2007,9(8):652-661.
[5]
Veiga JP,Cooper PA,Pors K,et al.Use of the hollow fiber assay for the evaluation of DNA damaging agents[J].J Pharmacol Toxicol Methods,2011,64(3):226-232.
Abstract: Objective To evaluated the inhibitory effect of garcinia glycosides on growth of 8 kinds of human tumor cells in vivo by hollow fiber assay and confirm the reliability of hollow fiber assay in anticancer effect by the nude mice xenograft test. Methods Hollow fibers containing tumor cells were inserted underneath the skin of the NOD/SCID mice.The fibers were collected from the mice on the day after the administration and subjected to the stable endpoint MTT assay.The tumor cells of HL-60 and B16 were subcutaneously implanted into the right flank of BALb /c nude mice.The positive control group was treated with cyclophosphamide.Each group was administered for 10 days.24 hours after the last administration, the mice were sacrificed and the tumors were excised and weighted, the inhibition rate of tumor growth was calculated. Results The high-dose group of 8 mg/(kg·d), middle dose group of 4 mg/(kg·d) of garcinia glycosides were measured by hollow fiber assay and nude mice test significantly inhibited the in vivo growth of HL-60 and B16 comparing with those in the solvent control group(P<0.01). Conclusion As a new model by hollow fiber assay to evaluate the inhibitory effect of garcinia glycosides, the test results were basically the same with nude mice test results.It made the experiment more rapidly, accurately and economically.An instruction and reliable evidence for follow-up study of garcinia glycosides was provided in this study.
HUANG Fengai, ZHANG Junping. Research progress on active ingredients from traditional Chinese medicine as inhibitors of PD-1/PD-L1 of cancer immune checkpoint[J]. Journal of Pharmaceutical Practice and Service, 2023, 41(5): 277-283, 290. doi: 10.12206/j.issn.2097-2024.202208079
Citation:
DENG Jingjing, JI Shengjun, CHEN Ye, LIN Xiaolin, WANG Rui, XU Lifeng. Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(4): 266-269. doi: 10.3969/j.issn.1006-0111.2014.04.007
Hollingshead MG,Alley MC, Camalier RF,et al.In vivo cultivation of tumor cells in hollow fibers[J].Life Sci,1995,57(2):131-141.
[3]
Lee KH,Rhee KH.Correlative effect between in vivo hollow fiber assay and xenografts assay in drug screening[J].Cancer Res Treat,2005,37(3):196-200.
[4]
Zhang GJ,Chen TB,Bednar B,et al.Optical imaging of tumor cells in hollow fibers:evaluation of the antitumor activities of anticancer drugs and target validation[J].Neoplasia,2007,9(8):652-661.
[5]
Veiga JP,Cooper PA,Pors K,et al.Use of the hollow fiber assay for the evaluation of DNA damaging agents[J].J Pharmacol Toxicol Methods,2011,64(3):226-232.
HUANG Fengai, ZHANG Junping. Research progress on active ingredients from traditional Chinese medicine as inhibitors of PD-1/PD-L1 of cancer immune checkpoint[J]. Journal of Pharmaceutical Practice and Service, 2023, 41(5): 277-283, 290. doi: 10.12206/j.issn.2097-2024.202208079
HUANG Fengai, ZHANG Junping. Research progress on active ingredients from traditional Chinese medicine as inhibitors of PD-1/PD-L1 of cancer immune checkpoint[J]. Journal of Pharmaceutical Practice and Service, 2023, 41(5): 277-283, 290. doi: 10.12206/j.issn.2097-2024.202208079
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