Screening of active compounds of pro-angiogenic in Shexiang Baoxin pill

LV Chao; HUANG Huimei; CHANG Wanlin; LIU Runhui

doi:10.3969/j.issn.1006-0111.2014.05.007

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Objective To screen and identify the main pro-angiogenic compounds of Shexiang Baoxin pill (SBP) presenting in the plasma. Methods The pro-angiogenic effects of SBP and its compounds absorbed into blood were measured by the cell proliferation and cell migration assays by xCELLigence. And the cell tube formation and rat aortic ring models were established to evaluate their pro-angiogenic effect. Results SBP(10^-4~10^-2 μg/ml), ginsenoside Rg3(1~10 μmol/L) and ginsenoside Rh2(1~10 μmol/L)significantly stimulated human umbilical vein endothelial cells (HUVECs) proliferation, migration and tube-like structures formation at different concentrations (P<0.05). In addition, compared to the control group, only the high concentration group of SBP (10^-2 μg/ml), Rg3(10 μmol/L) and Rh2(10 μmol/L)could induce endothelial cell sprouting from the aortic ring(P<0.05). Conclusion SBP, ginsenosideRg3 and Rh2 exhibited significantly pro-angiogenic effect in vitro.

Screening of active compounds of pro-angiogenic in Shexiang Baoxin pill

School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian 350108, China

Received Date: 2014-04-03

Rev Recd Date: 2014-06-27

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Abstract: Objective To screen and identify the main pro-angiogenic compounds of Shexiang Baoxin pill (SBP) presenting in the plasma. Methods The pro-angiogenic effects of SBP and its compounds absorbed into blood were measured by the cell proliferation and cell migration assays by xCELLigence. And the cell tube formation and rat aortic ring models were established to evaluate their pro-angiogenic effect. Results SBP(10^-4~10^-2 μg/ml), ginsenoside Rg3(1~10 μmol/L) and ginsenoside Rh2(1~10 μmol/L)significantly stimulated human umbilical vein endothelial cells (HUVECs) proliferation, migration and tube-like structures formation at different concentrations (P<0.05). In addition, compared to the control group, only the high concentration group of SBP (10^-2 μg/ml), Rg3(10 μmol/L) and Rh2(10 μmol/L)could induce endothelial cell sprouting from the aortic ring(P<0.05). Conclusion SBP, ginsenosideRg3 and Rh2 exhibited significantly pro-angiogenic effect in vitro.

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Reference (16)

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Screening of active compounds of pro-angiogenic in Shexiang Baoxin pill

doi: 10.3969/j.issn.1006-0111.2014.05.007

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