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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2014  >  32(5): 354-356

JIANG Nan, YANG Yongge, SONG Lixue, XU Xueting. Determination of febuxostate in human plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(5): 354-356. doi: 10.3969/j.issn.1006-0111.2014.05.010
Citation: JIANG Nan, YANG Yongge, SONG Lixue, XU Xueting. Determination of febuxostate in human plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(5): 354-356. doi: 10.3969/j.issn.1006-0111.2014.05.010
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Determination of febuxostate in human plasma by LC-MS-MS

doi: 10.3969/j.issn.1006-0111.2014.05.010

  • Department of Pharmacy, General Hospital, Beijing Military Region, Beijing 100700, China

  • Received Date: 2013-08-08
  • Rev Recd Date: 2014-04-14
  • Objective To establish a LC-MS-MS method for determining febuxostate in human plasma. Methods Febuxostate added into blank plasma was sedimented by acetonitrile, and the supernatant was determined by LC-MS-MS. Analytical column was Thermo Biobasic-8, 5 μm, 50 mm×2.1 mm(ID). The mobile phase consisted of acetonitrile-10 mmol/L ammonium acetate (0.05% acid=70:30 at a flow rate of 0.2 ml/min. Mass spectrum conditions:ESI-was performed in the SRM mode using target ions m/z 315→271 (10 eV)(febuxostate), m/z 360→274 (18 eV)(bezafibrate), SP 3 500 kV, SGP 10 Arb, AGP45 Arb, TEM 270℃. Results The calibration curve was linear over the range of 10-8 000 μg/L. The LLOQ of Febuxostate in plasma was 10 μg/L.The extracted recovery was >85%.The intra-and inter-day RSD were <15%. Conclusion The method was sensitive, simple and accurate to determinate febuxostate plasma concentration and to study pharmacokinetics of febuxostate.
  • [1] Mayer MD, Khosravan R, Vernillet L, et al. Pharmacokinetics and pharmacodynamics of febuxostal, a new non-purine selective inhibitor of xanthine oxidase, in subjects with renal impairment[J]. Am J Ther, 2005,12(1):22-34.
    [2] 张文丽,程 航,阳国平.高效液相-荧光法测定人血浆中非布司他的浓度及其人体药动学研究[J].临床药理学,2011,16(10):1148-1152.
    [3] 施 政,刘 健,申屠建中,等. 非布司他在中国健康人体的生物等效性[J].中国临床药理学杂志,2013,162(4):276-279.
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Determination of febuxostate in human plasma by LC-MS-MS

doi: 10.3969/j.issn.1006-0111.2014.05.010
  • Department of Pharmacy, General Hospital, Beijing Military Region, Beijing 100700, China
  • Received Date: 2013-08-08
  • Rev Recd Date: 2014-04-14

Abstract: Objective To establish a LC-MS-MS method for determining febuxostate in human plasma. Methods Febuxostate added into blank plasma was sedimented by acetonitrile, and the supernatant was determined by LC-MS-MS. Analytical column was Thermo Biobasic-8, 5 μm, 50 mm×2.1 mm(ID). The mobile phase consisted of acetonitrile-10 mmol/L ammonium acetate (0.05% acid=70:30 at a flow rate of 0.2 ml/min. Mass spectrum conditions:ESI-was performed in the SRM mode using target ions m/z 315→271 (10 eV)(febuxostate), m/z 360→274 (18 eV)(bezafibrate), SP 3 500 kV, SGP 10 Arb, AGP45 Arb, TEM 270℃. Results The calibration curve was linear over the range of 10-8 000 μg/L. The LLOQ of Febuxostate in plasma was 10 μg/L.The extracted recovery was >85%.The intra-and inter-day RSD were <15%. Conclusion The method was sensitive, simple and accurate to determinate febuxostate plasma concentration and to study pharmacokinetics of febuxostate.

JIANG Nan, YANG Yongge, SONG Lixue, XU Xueting. Determination of febuxostate in human plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(5): 354-356. doi: 10.3969/j.issn.1006-0111.2014.05.010
Citation: JIANG Nan, YANG Yongge, SONG Lixue, XU Xueting. Determination of febuxostate in human plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(5): 354-356. doi: 10.3969/j.issn.1006-0111.2014.05.010
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