GONG Yingzhen, ZHENG Chengjian, QIN Luping. Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011
| Citation:
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GONG Yingzhen, ZHENG Chengjian, QIN Luping. Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011
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Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments
- 1.
School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- 2.
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- Received Date: 2013-10-17
- Rev Recd Date:
2014-03-17
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Abstract
Objective To optimize the extraction technology of total falvonoids from Hypericum sampsonii. Methods On the basis of single factor test, solid-liquid ratio, ethanol concentration, extraction time, extraction temperature and extraction times on the extraction rate were studied by taking the content of total falvonoids as indexes. Orthogonal test was conducted to establish the extraction technology of total falvonoids from Hypericum sampsonii. Results The influence degree of factors on the extraction rate of total falvonoids was in the order of ethanol concentration, solid-liquid ratio, extraction times, and temperature.The optimum extraction condition was confirmed as follows: ethanol concentration of 70%, extraction temperature of 60 ℃, and extraction times of 3×1.5 h, solid to liquid ratio of 120. Under this condition,the average extraction rate of total falvonoids reached 2.38%. Conclusion The test was reasonable in design and credible in findings, and was easily operated, which could provide scientific basis for the batch extraction of total flavonoids from Hypericum sampsonii.
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