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LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012
Citation:
QIAO Liye, FANG Li, CAO Yanli, LU Yin, SU Hua, WANG Shudong. Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablets by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 438-440. doi: 10.3969/j.issn.1006-0111.2015.05.015
Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablets by HPLC
Department of Preparation, Nanjing General Hospital of Nanjing Military Region, PLA, Nanjing 210002, China
Received Date: 2014-05-27
Rev Recd Date:
2014-07-30
Abstract
Objective To establish method of the determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablet,for the production of quality protection. Methods HPLC was performed on a Lichrospher-C18 column(250 mm×4.6mm,5 μm)with the mobile phase of methanol-0.1% H3PO4 (70:30).The flow rate was 1.0 ml/min,the column temperature was at 35 ℃ and the detection wavelength was 254 nm. Results The linear rang of aloe-emodin,rhein,emodin,chrysophanol and physcion were obtained between 2.30-18.4 μg/ml ( r=1.0),2.930-23.44 μg/ml( r=1.0),5.00-40.0 μg/ml ( r=1.0),14.870-118.96 μg/ml( r= 0.9998 ) and 7.410-59.28 μg/ml(r=0.999 9),the average recoveries were 100.16%,102.91%,99.76%,100.32%,100.44%,RSD were 1.58%,1.27%,1.67%,1.33%,1.03% (n=9). Conclusion The improved method was accurate and feasible which could be used convenient in quality control.
Abstract: Objective To establish method of the determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablet,for the production of quality protection. Methods HPLC was performed on a Lichrospher-C18 column(250 mm×4.6mm,5 μm)with the mobile phase of methanol-0.1% H3PO4 (70:30).The flow rate was 1.0 ml/min,the column temperature was at 35 ℃ and the detection wavelength was 254 nm. Results The linear rang of aloe-emodin,rhein,emodin,chrysophanol and physcion were obtained between 2.30-18.4 μg/ml ( r=1.0),2.930-23.44 μg/ml( r=1.0),5.00-40.0 μg/ml ( r=1.0),14.870-118.96 μg/ml( r= 0.9998 ) and 7.410-59.28 μg/ml(r=0.999 9),the average recoveries were 100.16%,102.91%,99.76%,100.32%,100.44%,RSD were 1.58%,1.27%,1.67%,1.33%,1.03% (n=9). Conclusion The improved method was accurate and feasible which could be used convenient in quality control.
LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012
Citation:
QIAO Liye, FANG Li, CAO Yanli, LU Yin, SU Hua, WANG Shudong. Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablets by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 438-440. doi: 10.3969/j.issn.1006-0111.2015.05.015
LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012
LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012