Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 34 Issue 3
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(3): 223-226,277

XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008
Citation: XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008
shu

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

doi: 10.3969/j.issn.1006-0111.2016.03.008
  • 1.

    AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China;School of Life Sciences, Fudan University, Shanghai 200438, China

  • 2.

    AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China

  • 3.

    School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

  • Received Date: 2015-06-26
  • Rev Recd Date: 2015-10-21
  • Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density(OD) in 490 nm and inhibition rate(GI50)was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation assay, which except for NUGC-4 and OCUM-1 did not showed obvious anti-proliferative effect, the other 24 human gastric cell lines showed some anti-proliferative effects, especially for 7 cell lines(NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU-216 and SNU-5) showing strong potency, with their GI50 less than 50 μg/ml. Conclusion FIPs showed strong anti-proliferative effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti-gastric cancer drugs.
  • [1] 苏恺琪, 王雪飞, 周选围. 黑灵芝免疫调节蛋白基因的克隆和生物信息学分析[J]. 上海交通大学学报(农业科学版), 2012, 30(1):65-71.
    [2] Li F, Wen H, Zhang Y, et al. Purification and characterization of a novel immunomodulatory protein from the medicinal mushroom Trametes versicolor [J]. Sci China Life Sci, 2011, 54(4):379-385.
    [3] Wang XF, Li QZ, Bao TW, et al. In vitro rapid evolution of fungal immunomodulatory proteins by DNA family shuffling[J]. Appl Microbiol Biotechnol, 2013, 97(6):2455-2465.
    [4] Li QZ, Huang L, Wang XF, et al. Fungal immunomodulatory protein from Flammulina velutipes induces cytokine gene expression in mouse spleen cells[J]. Curr Top Nutraceut R, 2011, 9(3):111-118.
    [5] Li QZ, Wang XF, Zhou XW. Recent status and prospects of the fungal immunomodulatory protein family[J]. Crit Rev Biotechnol, 2011, 31(4):365-375.
    [6] 王晓林, 梁重阳, 李泓睿, 等. 灵芝免疫调节蛋白(rLZ-8)诱导K562细胞发生细胞核介导的细胞凋亡的研究[J]. 中国免疫学杂志, 2010, 26(7):616-618.
    [7] Liao CH, Hsiao YM, Lin CH, et al. Induction of premature senescence in human lung cancer by fungal immunomodulatory protein from Ganoderma tsugae[J]. Food Chem Toxicol, 2008, 46(5):1851-1859.
    [8] Cong WR, Xu H, Liu Y, et al. Production and functional characterization of a novel fungal immunomodulatory protein FIP-SN shuffled from two genes of Ganoderma species[J]. App Microbiol Biotechnol, 2014, 98(13):5967-5975.
    [9] 刘建文.抗肿瘤药理实验方法[M].北京:化学工业出版社,2010:29.
    [10] 司徒镇强, 吴军正. 细胞培养[M]. 北京:世界图书出版社西安公司, 1996:135.
    [11] Tanaka M, Obata T, Sasaki T.Evaluation of antitumour effects of docetaxel (Taxotere) on human gastric cancers in vitro and in vivo [J]. Eur J Cancer, 1996, 32A (2):226-230.
    [12] Wang XF, Su KQ, Bao TW, et al. Immunomodulation effects of fungal proteins[J]. Curr Top Nutraceut R, 2012, 10(1):1-11.
    [13] Kino K, Yamashita A, Yamaoka K, et al. Isolation and characterization of a new immunomedulatory protein, ling zhi-8(LZ-8), from Ganoderma lucidum [J]. J Biol Chem, 1989, 264:472-478.
    [14] Lin WH, Huang CH, Hsu CI, et al. Dimerization of the N-terminal amphipathic alpha-helix domain of the fungal immunomodulatory protein from Ganoderma tsugae (Fip-gts) defined by a yeast two-hybrid system and site-directed mutagenesis[J]. J Biol Chem, 1997, 272(32):20044-20048.
    [15] Ishiyama M, Tominaga H, Shiga M, et al. Novel cell proliferation and cytotoxicity assays using a tetrazolium salt that produces a comparison of multiple cytotoxicity assays 61 water-soluble formazan dye[J]. In Vitro Toxicol, 1995, 8(2):187-190.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(3273) PDF downloads(7) Cited by()

Related
Proportional views

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

doi: 10.3969/j.issn.1006-0111.2016.03.008
  • 1. AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China;School of Life Sciences, Fudan University, Shanghai 200438, China
  • 2. AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China
  • 3. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
  • Received Date: 2015-06-26
  • Rev Recd Date: 2015-10-21

Abstract: Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density(OD) in 490 nm and inhibition rate(GI50)was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation assay, which except for NUGC-4 and OCUM-1 did not showed obvious anti-proliferative effect, the other 24 human gastric cell lines showed some anti-proliferative effects, especially for 7 cell lines(NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU-216 and SNU-5) showing strong potency, with their GI50 less than 50 μg/ml. Conclusion FIPs showed strong anti-proliferative effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti-gastric cancer drugs.

XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008
Citation: XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008
shu

    HTML

Reference (15)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return