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Volume 34 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(3): 249-251,254

GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
Citation: GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
shu

Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra

doi: 10.3969/j.issn.1006-0111.2016.03.014
  • 1.

    School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China

  • 2.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2014-08-14
  • Rev Recd Date: 2014-12-09
  • Objective To establish the HPLC fingerprint of Ginseng Radix et Rhizoma Rubra for its quality control. Methods Sharpsil-T C18 column(4.6 mm×250 mm, 5 μm)was used with acetonitrile-water in gradient elution mode. The flow rate was 1.0 ml/min, the column temperature was maintained at 30℃, and the detective wavelength was 203 nm. Results 24 co-possessing peaks were selected as fingerprint peaks and the similarities between each of the ten areas and the standard chromatographic fingerprints of Panax ginseng Rubra were calculated while Rb1 peak selected as the reference peak. The similarity was more than 0.940. Eleven chemical compounds were identified by comparing the reference substance. Conclusion The method could be used for the quality control of Panax ginseng Rubra with good precision, stability, and reproducibility.
  • [1] 国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社,2010:143.
    [2] 罗国安,王明义,曹进,等.建立我国现代中药质量标准体系的研究[J].世界科学技术-中药现代化,2002,4(4):5-11.
    [3] 刘唯芬,熊英,幕善学,等.红参HPLC指纹图谱研究[J].辽宁中医杂志,2009,36(7):1178-1179.
    [4] 蔡萍,肖娟,张水寒,等.红参超微饮片的指纹图谱研究[J].中华中医药杂志,2011,26(7):1513-1515.
    [5] 郑重,宋凤瑞,刘淑莹,等.人参、红参皂苷类成分指纹图谱研究[J].质谱学报,2012,33(6):327-333.
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Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra

doi: 10.3969/j.issn.1006-0111.2016.03.014
  • 1. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China
  • 2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2014-08-14
  • Rev Recd Date: 2014-12-09

Abstract: Objective To establish the HPLC fingerprint of Ginseng Radix et Rhizoma Rubra for its quality control. Methods Sharpsil-T C18 column(4.6 mm×250 mm, 5 μm)was used with acetonitrile-water in gradient elution mode. The flow rate was 1.0 ml/min, the column temperature was maintained at 30℃, and the detective wavelength was 203 nm. Results 24 co-possessing peaks were selected as fingerprint peaks and the similarities between each of the ten areas and the standard chromatographic fingerprints of Panax ginseng Rubra were calculated while Rb1 peak selected as the reference peak. The similarity was more than 0.940. Eleven chemical compounds were identified by comparing the reference substance. Conclusion The method could be used for the quality control of Panax ginseng Rubra with good precision, stability, and reproducibility.

GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
Citation: GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
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