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ZHANG Yuanlin, SONG Kai, SUN Rui, SHU Fei, SHU Lixin, YANG Zhangwei. Review of drug utilization research based on real-world data[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(6): 238-243. doi: 10.12206/j.issn.2097-2024.202312010
Citation:
SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD
Food and Drug Inspection Center of Tangshan, Tangshan 063020, China
2.
The Children's Hospital of Hebei Province, Shijiazhuang 050030, China
Received Date: 2016-03-21
Rev Recd Date:
2016-05-26
Abstract
Objective To develop a new high performance liquid chromatography (HPLC) coupled with Evaporative Light Scattering Detector (ELSD) method for simultaneous determination of two indicative components (peimine and peiminine) in Xiaoer Baotaikang granules. Methods The analysis was performed on a Thermo C18 column (250 mm×4.6 mm, 5 μm) by using a mobile phase consisted of acetonitrile-water and diethylamine(65:35:0.03), and the flow rate was 1.0 ml/min; ELSD parameters:the temperature of drift tube was 85℃ and the carrier gas flow rate was 2.2 L/min. Results The linear ranges of peimine and peiminine were 0.586~5.860 μg (r=0.9996) and 0.564~5.640 μg (r=0.9999), respectively. The average recovery rate for peimine and peiminine was 99.44% (RSD was 0.7%) and 98.56% (RSD was 1.0%),respectively. Conclusion s The method is sample,accurate and reproducible, which could be used for the quality control of Xiaoer Baotaikang granules.
Abstract: Objective To develop a new high performance liquid chromatography (HPLC) coupled with Evaporative Light Scattering Detector (ELSD) method for simultaneous determination of two indicative components (peimine and peiminine) in Xiaoer Baotaikang granules. Methods The analysis was performed on a Thermo C18 column (250 mm×4.6 mm, 5 μm) by using a mobile phase consisted of acetonitrile-water and diethylamine(65:35:0.03), and the flow rate was 1.0 ml/min; ELSD parameters:the temperature of drift tube was 85℃ and the carrier gas flow rate was 2.2 L/min. Results The linear ranges of peimine and peiminine were 0.586~5.860 μg (r=0.9996) and 0.564~5.640 μg (r=0.9999), respectively. The average recovery rate for peimine and peiminine was 99.44% (RSD was 0.7%) and 98.56% (RSD was 1.0%),respectively. Conclusion s The method is sample,accurate and reproducible, which could be used for the quality control of Xiaoer Baotaikang granules.
ZHANG Yuanlin, SONG Kai, SUN Rui, SHU Fei, SHU Lixin, YANG Zhangwei. Review of drug utilization research based on real-world data[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(6): 238-243. doi: 10.12206/j.issn.2097-2024.202312010
Citation:
SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
ZHANG Yuanlin, SONG Kai, SUN Rui, SHU Fei, SHU Lixin, YANG Zhangwei. Review of drug utilization research based on real-world data[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(6): 238-243. doi: 10.12206/j.issn.2097-2024.202312010
ZHANG Yuanlin, SONG Kai, SUN Rui, SHU Fei, SHU Lixin, YANG Zhangwei. Review of drug utilization research based on real-world data[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(6): 238-243. doi: 10.12206/j.issn.2097-2024.202312010
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