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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(6): 530-533

XU Shaolan, ZHAO Dan, MAO Junqin. Study on quality control of Jianpibushen granules[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 530-533. doi: 10.3969/j.issn.1006-0111.2016.06.012
Citation: XU Shaolan, ZHAO Dan, MAO Junqin. Study on quality control of Jianpibushen granules[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 530-533. doi: 10.3969/j.issn.1006-0111.2016.06.012
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Study on quality control of Jianpibushen granules

doi: 10.3969/j.issn.1006-0111.2016.06.012

  • Department of Pharmacy, No. 85 Hospital of PLA, Shanghai 200052, China

  • Received Date: 2015-09-01
  • Rev Recd Date: 2015-10-21
  • Objective To establish a quality standard for Jianpibushen granules. Methods The five main ingredients of the formulation:Astragalus membranaceus, Salvia miltiorrhiza Bunge, Codonopsis pilosula, Citrus reticulata Blanco and Paeonia lactiflora Pall, were identified by thin layer chromatography (TLC) respectively. The content of peoniflorin in Paeonia lactiflora was determined by high performance liquid chromatography (HPLC). The separation was performed on Agilent Eclipse Plus C18 column(4.6 mm×250 mm, 5 μm). The mobile phase was acetonitrile and 0.1% potassium dihydrogen phosphate solution for gradient elution. The flow rate was 1 ml/min, the column temperature was 25℃, the UV detection was performed at 230 nm. Results The spots in TLC were clear without interference in negative control. A good linear relationship was shown within the range of 8.676-277.632 μg/ml for peoniflorin (r=0.999 9). Conclusion This method is simple, accurate and reproducible, and can be used as an effective quality control of Jianpibushen granules.
  • [1] 国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2010:70,176,264,283-284,282-283.
    [2] 吴笛,王德勤,李楚源.复方丹参片薄层色谱鉴别方法研究[J].药物分析杂志,2012,32(9):1658-1660.
    [3] 赵越平,张晓楠,张延风,等.益胃胶囊中部分有效成分的薄层鉴别及芍药苷的HPLC测定[J].第四军医大学学报,2001,22(17):1583-1585.
    [4] 李伯军,付艳敏,李铁强,等.HPLC法测定痢必灵片中芍药苷的含量[J].中国药事,2007,21(2):110-112.
    [5] 尹宁宁,徐华玲,徐丽华.白芍中芍药苷含量测定影响因素的实验研究[J]. 中国医药导报,2009(11):43-44.
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Study on quality control of Jianpibushen granules

doi: 10.3969/j.issn.1006-0111.2016.06.012
  • Department of Pharmacy, No. 85 Hospital of PLA, Shanghai 200052, China
  • Received Date: 2015-09-01
  • Rev Recd Date: 2015-10-21

Abstract: Objective To establish a quality standard for Jianpibushen granules. Methods The five main ingredients of the formulation:Astragalus membranaceus, Salvia miltiorrhiza Bunge, Codonopsis pilosula, Citrus reticulata Blanco and Paeonia lactiflora Pall, were identified by thin layer chromatography (TLC) respectively. The content of peoniflorin in Paeonia lactiflora was determined by high performance liquid chromatography (HPLC). The separation was performed on Agilent Eclipse Plus C18 column(4.6 mm×250 mm, 5 μm). The mobile phase was acetonitrile and 0.1% potassium dihydrogen phosphate solution for gradient elution. The flow rate was 1 ml/min, the column temperature was 25℃, the UV detection was performed at 230 nm. Results The spots in TLC were clear without interference in negative control. A good linear relationship was shown within the range of 8.676-277.632 μg/ml for peoniflorin (r=0.999 9). Conclusion This method is simple, accurate and reproducible, and can be used as an effective quality control of Jianpibushen granules.

XU Shaolan, ZHAO Dan, MAO Junqin. Study on quality control of Jianpibushen granules[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 530-533. doi: 10.3969/j.issn.1006-0111.2016.06.012
Citation: XU Shaolan, ZHAO Dan, MAO Junqin. Study on quality control of Jianpibushen granules[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 530-533. doi: 10.3969/j.issn.1006-0111.2016.06.012
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