Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 35 Issue 5
. 
Turn off MathJax
Article Contents
Abstract
References
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2017  >  35(5): 402-406,426

QIAO Jin, CHEN Min, DOU Zhihua, XU Jiliang, WU Feng, MENG Guoliang. Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 402-406,426. doi: 10.3969/j.issn.1006-0111.2017.05.004
Citation: QIAO Jin, CHEN Min, DOU Zhihua, XU Jiliang, WU Feng, MENG Guoliang. Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 402-406,426. doi: 10.3969/j.issn.1006-0111.2017.05.004
shu

Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney

doi: 10.3969/j.issn.1006-0111.2017.05.004
  • 1.

    Department of Pharmacy, Third Hospital Affiliated to Nantong University, Nantong 226006, China

  • 2.

    Department of Pharmacology, Medical College, Nantong University, Nantong 226001, China

  • Received Date: 2017-05-18
  • Rev Recd Date: 2017-06-26
  • Objective To study the protective effect of Rhein on the kidney of type 2 diabetic rats induced by high fat diet. Methods A rat model of type 2 diabetes was induced by high fat diet combined with low dose streptozotocin 35 mg/kg. The diabetic rats were randomly divided into diabetes group, Low, middle and high rhein dose groups (50,100,150 mg/kg), metformin group (300 mg/kg) and normal control group. Blood glucose and urine micro albumin were measured at 0, 2, 4 and 8 weeks respectively. Serum creatinine, urea nitrogen, total cholesterol and triglyceride were measured at 8 weeks. HE staining was used to observe the pathological changes of renal tissue. Effects of rhein on the expression of transforming growth factor-β1 and Smad3 protein in renal tissue of diabetic rats were detected with Western Blot. Results The blood glucose and urine micro albumin in model group were significantly higher than those in normal control group. Each rhein dose group exhibited reduced blood glucose and urinary micro albumin in diabetic rats. The high rhein dose group showed significant reduction of blood glucose and urine micro albumin in diabetic rats (P<0.05). Compared with model group, rhein reduced the serum Cr, BUN, TC and TG values in each dose group, most significantly in the high rhein dose group (P<0.05). The obvious pathological changes of renal tissue in model group were observed with most improved changes in the high rhein dose group. The expression of TGF-β1 and Smad3 protein in renal tissue of diabetic rats decreased significantly (P<0.05). Conclusion Rhein has preventive effect on diabetic nephropathy. The mechanism may relate to the improvement of renal function, regulation of blood lipid and down regulation of TGF-β1 and Smad3 protein expression in renal tissue.
  • [1] Sheng NP,Hui HZ,Ai XF,et al. Protection of rhein on IgA nephropathy mediated by inhibition of fibronectin expression in rats［J］. Indian J Pharmacol,2013,45(2):174-179.
    [2] Xu Y,Wang L,He J,et al. Prevalence and control of diabetes in Chinese adults［J］.JAMA,2013,310(9):948-959.
    [3] 陈才铭,张苗苗,胡利明.大黄酸对肥胖糖尿病大鼠肾皮质PPARγ_和TGF-β1表达的影响［J］.中药材,2015,38(4):810-812.
    [4] 吴影懿,段俗言,钱 军,等.大黄酸对糖尿病小鼠肾脏的保护作用［J］.江苏医药,2015,41 (16):1864-1866.
    [5] 陈卫东,常保超,张 燕,等.大黄酸增加2型糖尿病大鼠肾组织SIRT1的表达［J］.细胞与分子免疫学杂志,2015,31(5):615-619.
    [6] Geng CC,Chen GR,Wu QJ,et al.The molecular mechanism of rhein in diabetic nephropathy［J］.Evid Based Complement Alternat Med,2014,20(14):487-497.
    [7] 乔 进,窦志华,吴 锋,等.灵芝多糖联合二甲双胍对2型糖尿病大鼠心肌AGEs及CTGF的影响及其机制［J］.中国药理学通报,2014,30(4):536-541.
    [8] 乔 进,窦志华,吴 锋,等.灵芝多糖联合二甲双胍预防糖尿病大鼠主动脉病变及对VEGF表达的影响［J］.中国药理学通报,2014,30(8):1079-1084.
    [9] 乔 进,窦志华,吴 锋,等.灵芝多糖联合二甲双胍对2型糖尿病大鼠心肌结构及血流动力学的影响［J］.中国药理学通报,2016,32(7):1012-1016.
    [10] 陈 颖,刘竹影,周福兴,等.双丹口服液对大鼠糖尿病肾病的作用研究［J］.西北药学杂志,2016,31(1):56-61.
    [11] Chen Y,Liu Z Y,Zhou F X,et al. Effects of Shuangdan Oral Liquid on diabetic nephropathy rat models［J］.Northwest Pharm J,2016,31(1):56-61.
    [12] 李梦莹,谢春光,王晓灿.大黄酸治疗糖尿病肾病实验研究进展［J］.湖南中医杂志,2015,31(10):172-173.
    [13] Loeffler I,Hopfer U,Koczan D,et al. Type VIII collagen modulater TGF-β1- induced proliferation of mesangial cells［J］.J Am Soc Nephrol, 2011,22(4):649-663.
    [14] Overstreet JM,Samara Koon R,Meldrum KK,et al. Redox control of p53 in the transcriptional regulation of TGF-betal target genes through SMAD cooperativity ［J］.Cell Signal,2014,26(7):1427-1436.
    [15] Kim D,Lee AS,Jung YJ,et al. Tamoxifen ameliorates renal tubulointerstitial fibrosis by modulation of estrogen receptor alpha-mediated transforming growth factor-betal/Smad signaling pathway［J］.Nephrol Dial Transplant,2014,29(11):2043-2053.
    [16] Samarakoon R,Overstreet JM,Higgins PJ.TGF-beta signal in tissue fibrosis:redox controls,target genes and therapeutic opportunities［J］.Cell Signal,2013,25(1):264-268.
    [17] Zhang J,Zhang X,Xie F,et al.The regulation of TGF-beta/SMAD signaling by protein deubiquitination［J］.Protein Cell,2014,5(7):503-517.
    [18] Xie F,Zhang Z,van Dam H,et al. Regulation of TGF-beta superfamily signaling by SMAD mono-ubiquitination[j].Cells,2014,3(4):981-993.
  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-0402468Highcharts.com
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 79.4 %FULLTEXT: 79.4 %META: 17.2 %META: 17.2 %PDF: 3.4 %PDF: 3.4 %FULLTEXTMETAPDFHighcharts.com
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 33.2 %其他: 33.2 %Al Hufuf: 1.1 %Al Hufuf: 1.1 %China: 0.5 %China: 0.5 %Malvern: 0.4 %Malvern: 0.4 %United States: 0.2 %United States: 0.2 %Westbury: 0.4 %Westbury: 0.4 %[]: 0.2 %[]: 0.2 %上海: 2.7 %上海: 2.7 %东莞: 0.2 %东莞: 0.2 %临汾: 0.2 %临汾: 0.2 %佛山: 0.2 %佛山: 0.2 %北京: 5.4 %北京: 5.4 %南京: 0.2 %南京: 0.2 %哈尔滨: 0.4 %哈尔滨: 0.4 %圣路易斯: 0.4 %圣路易斯: 0.4 %天津: 0.2 %天津: 0.2 %广州: 0.2 %广州: 0.2 %张家口: 0.9 %张家口: 0.9 %成都: 0.4 %成都: 0.4 %杭州: 0.7 %杭州: 0.7 %格兰特县: 0.2 %格兰特县: 0.2 %桂林: 0.2 %桂林: 0.2 %武汉: 0.2 %武汉: 0.2 %漳州: 0.2 %漳州: 0.2 %芒廷维尤: 24.9 %芒廷维尤: 24.9 %西宁: 15.6 %西宁: 15.6 %西安: 0.2 %西安: 0.2 %运城: 0.4 %运城: 0.4 %郑州: 0.9 %郑州: 0.9 %长沙: 0.2 %长沙: 0.2 %长治: 0.2 %长治: 0.2 %驻马店: 8.6 %驻马店: 8.6 %黄冈: 0.7 %黄冈: 0.7 %其他Al HufufChinaMalvernUnited StatesWestbury[]上海东莞临汾佛山北京南京哈尔滨圣路易斯天津广州张家口成都杭州格兰特县桂林武汉漳州芒廷维尤西宁西安运城郑州长沙长治驻马店黄冈Highcharts.com

  • Cited by

    Periodical cited type(19)

    1. 陈智娴，乔进，陈霞，窦志华，徐济良. 基于抑制氧化应激与AGEs表达探讨大黄酸对2型糖尿病大鼠主动脉内皮功能的保护作用. 中国老年学杂志. 2024(21): 5294-5297 .
    2. 张湃，崔成姬，张守琳，张洪宝，刘洪凯，王子昆，李凡. TGF-β1/Smad3与自噬作用在糖尿病肾脏病中的作用机制及中药治疗. 中国老年学杂志. 2024(22): 5614-5617 .
    3. 庞健丽，钟润芬. 左归丸加减联合培哚普利叔丁胺片治疗气阴两虚夹瘀证早期糖尿病肾病临床观察. 中国实验方剂学杂志. 2023(01): 105-112 .
    4. 乔进，赵彦，陈霞，窦志华，孟国梁，吴锋，徐济良. 基于PI3K/Akt/FoxO1通路探讨大黄酸对2型糖尿病大鼠肾损伤的作用. 中成药. 2023(02): 609-613 .
    5. 刘月清，俞曼殊，单云，盛梅笑. 慢性肾脏病泄浊六法浅析. 中医药临床杂志. 2023(05): 862-866 .
    6. 古卓强，梁文辉. 大黄酸对革兰阴性菌的体外抑菌作用及其机制研究. 临床合理用药. 2023(24): 32-34+38 .
    7. 周其其，闵洁，刁婷婷，张雨晨，肖峰，要辉，白育庭. 山楂叶总黄酮对高脂血症肾损伤大鼠的保护作用. 医药导报. 2022(05): 608-615 .
    8. 刘琪文，杨德智，杨秀颖，吕扬，杜冠华. 大黄酸对糖尿病肾病的作用及其机制研究进展. 医药导报. 2022(06): 848-852 .
    9. 刘洋，郭璐萱，郝娜. 肾康注射液的药理作用及在慢性肾脏病3～5期临床治疗研究进展. 现代中药研究与实践. 2022(04): 98-102 .
    10. 赵奕，郑曙琴. 中药单体基于转化生长因子β1-Smad信号通路机制干预糖尿病肾病大鼠模型的系统评价和贝叶斯网状Meta分析. 中医临床研究. 2022(28): 135-141 .
    11. 汪四海，方朝晖，倪英群，赵进东，熊国慧，方舟，张竣玮，毕正. 丹蛭降糖胶囊对糖尿病肾病大鼠肾脏TGF-β1/Smad3信号通路和AGEs/RAGE水平的影响. 中华中医药杂志. 2021(04): 2019-2024 .
    12. 包玉华，良良，郭丽娟，郭德森. 蒙成药给喜古纳-3汤研究进展. 内蒙古民族大学学报(自然科学版). 2021(02): 154-159 .
    13. 段晓星，常永丽，张国光，潘雪. 基于Notch1-RBP-Jk/Msx2信号通路探讨大黄酸对糖尿病肾病主动脉钙化的作用及机制研究. 临床肾脏病杂志. 2020(01): 51-56 .
    14. 马凯玲，龚飞，李红. 通过干预炎症介质核转录因子转化生长因子研究益肾宁延缓大鼠肾间质纤维化的作用机制. 山西医药杂志. 2020(23): 3210-3213 .
    15. 殷文贤，赵萌，吴知桂，顾立，陈美娟. 盐酸小檗碱对2型糖尿病大鼠血管平滑肌细胞增殖、迁移的影响. 重庆医学. 2019(05): 756-759+764 .
    16. 周丽霞，张娜娜，王瑞琪. 黄连降糖方治疗热盛津伤型消渴病的药理学研究. 光明中医. 2019(07): 1135-1137 .
    17. 郑舟琴，杜宏. 大黄酸药理作用的新进展. 重庆医学. 2019(22): 3897-3901 .
    18. 徐利娟，马丽. 大黄抑制糖尿病肾病肾纤维化机制研究进展. 新疆中医药. 2018(04): 137-140 .
    19. 王晓芹，邓小燕，于晓斌，王海. 茶多酚通过降脂、抗炎、抗氧化以及调控TGF-β/Smad信号通路缓解2型糖尿病. 中药药理与临床. 2018(03): 46-50 .

    Other cited types(17)

通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(5744) PDF downloads(1015) Cited by(36)

Related
Proportional views

Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney

doi: 10.3969/j.issn.1006-0111.2017.05.004
  • 1. Department of Pharmacy, Third Hospital Affiliated to Nantong University, Nantong 226006, China
  • 2. Department of Pharmacology, Medical College, Nantong University, Nantong 226001, China
  • Received Date: 2017-05-18
  • Rev Recd Date: 2017-06-26

Abstract: Objective To study the protective effect of Rhein on the kidney of type 2 diabetic rats induced by high fat diet. Methods A rat model of type 2 diabetes was induced by high fat diet combined with low dose streptozotocin 35 mg/kg. The diabetic rats were randomly divided into diabetes group, Low, middle and high rhein dose groups (50,100,150 mg/kg), metformin group (300 mg/kg) and normal control group. Blood glucose and urine micro albumin were measured at 0, 2, 4 and 8 weeks respectively. Serum creatinine, urea nitrogen, total cholesterol and triglyceride were measured at 8 weeks. HE staining was used to observe the pathological changes of renal tissue. Effects of rhein on the expression of transforming growth factor-β1 and Smad3 protein in renal tissue of diabetic rats were detected with Western Blot. Results The blood glucose and urine micro albumin in model group were significantly higher than those in normal control group. Each rhein dose group exhibited reduced blood glucose and urinary micro albumin in diabetic rats. The high rhein dose group showed significant reduction of blood glucose and urine micro albumin in diabetic rats (P<0.05). Compared with model group, rhein reduced the serum Cr, BUN, TC and TG values in each dose group, most significantly in the high rhein dose group (P<0.05). The obvious pathological changes of renal tissue in model group were observed with most improved changes in the high rhein dose group. The expression of TGF-β1 and Smad3 protein in renal tissue of diabetic rats decreased significantly (P<0.05). Conclusion Rhein has preventive effect on diabetic nephropathy. The mechanism may relate to the improvement of renal function, regulation of blood lipid and down regulation of TGF-β1 and Smad3 protein expression in renal tissue.

QIAO Jin, CHEN Min, DOU Zhihua, XU Jiliang, WU Feng, MENG Guoliang. Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 402-406,426. doi: 10.3969/j.issn.1006-0111.2017.05.004
Citation: QIAO Jin, CHEN Min, DOU Zhihua, XU Jiliang, WU Feng, MENG Guoliang. Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 402-406,426. doi: 10.3969/j.issn.1006-0111.2017.05.004
shu

    HTML

Reference (18)

Catalog

  • HTML

版权所有：《药学实践与服务》编辑委员会

Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

网站备案号 沪ICP备05003363号-1

Supported by: Beijing Renhe Information Technology Co., Ltd.  

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return