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HE Xiaobo, ZHU Jiahui, GAO Fangyuan. Progress on pharmacokinetics of biotechnology drugs[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 394-399. doi: 10.3969/j.issn.1006-0111.2019.05.003
Citation:
CHEN Zongliang, LÜ Xiaoxia, CHEN Guiqian, CHEN Jie. One step assay for five components in Xianlinggubao capsule by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 444-446,452. doi: 10.3969/j.issn.1006-0111.2017.05.014
One step assay for five components in Xianlinggubao capsule by HPLC method
Jinhua Institute for Food and Drug Control, Jinhua 321000, China
2.
Jinhua Jindong City Food and Drug Administration, Jinhua 321000, China
Received Date: 2016-06-28
Rev Recd Date:
2016-11-01
Abstract
Objective To assay Icariine, Epimedin C, asperosaponin Ⅵ, psoralen and angelicin in Xianlinggubao capsules via multi-wavelength HPLC method. Methods Separation was carried out on Welch Ultimate® XB-C18 column. The mobile phase was acetonitrile-water system and a linear gradient elution was used. The column temperature was 30 ℃. The detection wavelength for Icariine, Epimedin C,asperosaponin Ⅵ was set at 212 nm, psoralen and angelicin at 246 nm. Results Five components reached baseline separation, the linearity was good when sample size was in the range of 0.008 2-0.328 μg for Icariine(r=0.999 5), 0.055 6-2.224 μg for Epimedin C (r=0.999 6), 0.144 1-5.764 μg for asperosaponin Ⅵ(r=0.999 6), 0.005 4-0.215 2 μg for psoralen(r=0.998 0), 0.006 6-0.265 6 μg for angelicin(r=0.998 5). The average recoveries were 97.59%, 98.58%, 98.11%, 97.86%, 98.22% respectively. The RSDs of recovery were all less than 2.0%. Conclusion This method is simple, accurate, with good separation, high sensitivity for the assay of multiple components in Xianlinggubao capsule.
Abstract: Objective To assay Icariine, Epimedin C, asperosaponin Ⅵ, psoralen and angelicin in Xianlinggubao capsules via multi-wavelength HPLC method. Methods Separation was carried out on Welch Ultimate® XB-C18 column. The mobile phase was acetonitrile-water system and a linear gradient elution was used. The column temperature was 30 ℃. The detection wavelength for Icariine, Epimedin C,asperosaponin Ⅵ was set at 212 nm, psoralen and angelicin at 246 nm. Results Five components reached baseline separation, the linearity was good when sample size was in the range of 0.008 2-0.328 μg for Icariine(r=0.999 5), 0.055 6-2.224 μg for Epimedin C (r=0.999 6), 0.144 1-5.764 μg for asperosaponin Ⅵ(r=0.999 6), 0.005 4-0.215 2 μg for psoralen(r=0.998 0), 0.006 6-0.265 6 μg for angelicin(r=0.998 5). The average recoveries were 97.59%, 98.58%, 98.11%, 97.86%, 98.22% respectively. The RSDs of recovery were all less than 2.0%. Conclusion This method is simple, accurate, with good separation, high sensitivity for the assay of multiple components in Xianlinggubao capsule.
HE Xiaobo, ZHU Jiahui, GAO Fangyuan. Progress on pharmacokinetics of biotechnology drugs[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 394-399. doi: 10.3969/j.issn.1006-0111.2019.05.003
Citation:
CHEN Zongliang, LÜ Xiaoxia, CHEN Guiqian, CHEN Jie. One step assay for five components in Xianlinggubao capsule by HPLC method[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 444-446,452. doi: 10.3969/j.issn.1006-0111.2017.05.014
HE Xiaobo, ZHU Jiahui, GAO Fangyuan. Progress on pharmacokinetics of biotechnology drugs[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 394-399. doi: 10.3969/j.issn.1006-0111.2019.05.003
HE Xiaobo, ZHU Jiahui, GAO Fangyuan. Progress on pharmacokinetics of biotechnology drugs[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 394-399. doi: 10.3969/j.issn.1006-0111.2019.05.003
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