Research on the determination of total flavonoids in compound Yinchen mixture

LIU Xiaoling; CAI Xiaohui; NI Xiaoxia; WANG Qingfen

doi:10.3969/j.issn.1006-0111.2017.06.016

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Objective To establish a method for the determination of the total flavonoids content in compound Yinchen mixture by UV spectrophotometry. Methods Using rutin as comparison, three coloration methods were studied to find the optimal assay method. Results The sample was detected at 508 nm wavelength by NaNO₂-Al(NO₃)₃-NaOH reaction with rutin as reference. The rutin content had a liner relationship in the range of 0.012 5-0.062 6 g/L (n=9,r=0.999 9), and the average recovery rate was 99.49% with RSD of 0.84%. Conclusion The NaNO₂-Al(NO₃)₃-NaOH coloration method is proved to be simple, quick, stable and reliable for the determination of total flavonoids in compound Yinchen mixture.

Research on the determination of total flavonoids in compound Yinchen mixture

No. 175 Hospital of PLA, Zhangzhou 363000, China

Received Date: 2017-05-11

Rev Recd Date: 2017-07-31

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Abstract: Objective To establish a method for the determination of the total flavonoids content in compound Yinchen mixture by UV spectrophotometry. Methods Using rutin as comparison, three coloration methods were studied to find the optimal assay method. Results The sample was detected at 508 nm wavelength by NaNO₂-Al(NO₃)₃-NaOH reaction with rutin as reference. The rutin content had a liner relationship in the range of 0.012 5-0.062 6 g/L (n=9,r=0.999 9), and the average recovery rate was 99.49% with RSD of 0.84%. Conclusion The NaNO₂-Al(NO₃)₃-NaOH coloration method is proved to be simple, quick, stable and reliable for the determination of total flavonoids in compound Yinchen mixture.

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Research on the determination of total flavonoids in compound Yinchen mixture

doi: 10.3969/j.issn.1006-0111.2017.06.016

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