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ZHOU Jiao, ZHENG Jianyu, WANG Sizhen, YANG Feng. Progress on mRNA tumor vaccine with non-viral delivery system[J]. Journal of Pharmaceutical Practice and Service, 2025, 43(3): 109-116. doi: 10.12206/j.issn.2097-2024.202410034
Citation:
CHAI Ruiping, LU Juan, ZHAO Ying, WANG Xiaojing, LÜ Xinkai, CHEN Xi. Establishment of HPLC fingerprint for Wenjing Tang[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006
Establishment of HPLC fingerprint for Wenjing Tang
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China
2.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;Shandong University of Chinese Medicine, Jinan 250355, China
Received Date: 2017-12-12
Rev Recd Date:
2018-06-21
Abstract
Objective To establish chromatographic fingerprint for Wenjing Tang by HPLC-DAD. Methods The fingerprint was determined by Waters Xselect HSS T3 column (4.6 mm×250 mm,5μm) with acetoniteile-0.1% formic acid solution as mobile phase for gradient elution. The temperature of column was 25℃ at a flow rate of 0.8 ml·min-1. The detective wavelength was set at 254 nm and ammonium glycyrrhizinate was selected as the reference peak. The HPLC fingerprints of 10 batches of Wenjing Tang were analyzed and the similarity was evaluated with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine.Results There were 39 common peaks in the HPLC fingerprints of Wenjing Tang and 6 common peaks were determined by the relative time of the standard substance. The similarities of 10 batches were all above 0.98 and the deviations among these batches are very small. Conclusion The HPLC-DAD fingerprint method for Wenjing Tang is accurate, stable and reliable. The fingerprint is the characteristic of the overall chemical components in Wenjing Tang. It can be used as an effective method for the quality control of Wenjing Tang.
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China
2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;Shandong University of Chinese Medicine, Jinan 250355, China
Abstract: Objective To establish chromatographic fingerprint for Wenjing Tang by HPLC-DAD. Methods The fingerprint was determined by Waters Xselect HSS T3 column (4.6 mm×250 mm,5μm) with acetoniteile-0.1% formic acid solution as mobile phase for gradient elution. The temperature of column was 25℃ at a flow rate of 0.8 ml·min-1. The detective wavelength was set at 254 nm and ammonium glycyrrhizinate was selected as the reference peak. The HPLC fingerprints of 10 batches of Wenjing Tang were analyzed and the similarity was evaluated with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine.Results There were 39 common peaks in the HPLC fingerprints of Wenjing Tang and 6 common peaks were determined by the relative time of the standard substance. The similarities of 10 batches were all above 0.98 and the deviations among these batches are very small. Conclusion The HPLC-DAD fingerprint method for Wenjing Tang is accurate, stable and reliable. The fingerprint is the characteristic of the overall chemical components in Wenjing Tang. It can be used as an effective method for the quality control of Wenjing Tang.
ZHOU Jiao, ZHENG Jianyu, WANG Sizhen, YANG Feng. Progress on mRNA tumor vaccine with non-viral delivery system[J]. Journal of Pharmaceutical Practice and Service, 2025, 43(3): 109-116. doi: 10.12206/j.issn.2097-2024.202410034
Citation:
CHAI Ruiping, LU Juan, ZHAO Ying, WANG Xiaojing, LÜ Xinkai, CHEN Xi. Establishment of HPLC fingerprint for Wenjing Tang[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006
ZHOU Jiao, ZHENG Jianyu, WANG Sizhen, YANG Feng. Progress on mRNA tumor vaccine with non-viral delivery system[J]. Journal of Pharmaceutical Practice and Service, 2025, 43(3): 109-116. doi: 10.12206/j.issn.2097-2024.202410034
ZHOU Jiao, ZHENG Jianyu, WANG Sizhen, YANG Feng. Progress on mRNA tumor vaccine with non-viral delivery system[J]. Journal of Pharmaceutical Practice and Service, 2025, 43(3): 109-116. doi: 10.12206/j.issn.2097-2024.202410034