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Volume 36 Issue 6
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2018  >  36(6): 503-506

CHAI Ruiping, LU Juan, ZHAO Ying, WANG Xiaojing, LÜ Xinkai, CHEN Xi. Establishment of HPLC fingerprint for Wenjing Tang[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006
Citation: CHAI Ruiping, LU Juan, ZHAO Ying, WANG Xiaojing, LÜ Xinkai, CHEN Xi. Establishment of HPLC fingerprint for Wenjing Tang[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006
shu

Establishment of HPLC fingerprint for Wenjing Tang

doi: 10.3969/j.issn.1006-0111.2018.06.006
  • 1.

    Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China

  • 2.

    Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;Shandong University of Chinese Medicine, Jinan 250355, China

  • Received Date: 2017-12-12
  • Rev Recd Date: 2018-06-21
  • Objective To establish chromatographic fingerprint for Wenjing Tang by HPLC-DAD. Methods The fingerprint was determined by Waters Xselect HSS T3 column (4.6 mm×250 mm,5μm) with acetoniteile-0.1% formic acid solution as mobile phase for gradient elution. The temperature of column was 25℃ at a flow rate of 0.8 ml·min-1. The detective wavelength was set at 254 nm and ammonium glycyrrhizinate was selected as the reference peak. The HPLC fingerprints of 10 batches of Wenjing Tang were analyzed and the similarity was evaluated with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine. Results There were 39 common peaks in the HPLC fingerprints of Wenjing Tang and 6 common peaks were determined by the relative time of the standard substance. The similarities of 10 batches were all above 0.98 and the deviations among these batches are very small. Conclusion The HPLC-DAD fingerprint method for Wenjing Tang is accurate, stable and reliable. The fingerprint is the characteristic of the overall chemical components in Wenjing Tang. It can be used as an effective method for the quality control of Wenjing Tang.
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    [4] 唐卓,刘宇新. 温经汤治疗原发性寒凝血瘀型痛经的疗效观察80例[J]. 中医临床研究,2015, 7(31):95-97.
    [5] 张晓珍. 金匮温经汤治疗不孕不育的临床疗效观察[J]. 海峡药学,2015, 27(10):159.
    [6] 温慧玲,程齐来,吴龙火,等. 基于指纹图谱探讨吴茱萸不同炮制品成分的差异[J]. 中国实验方剂学杂志,2013, 19(5):76-80.
    [7] 王元清,韩彬,陈婷,等. 桂枝指纹图谱的建立及识别模式研究[J]. 中药材,2017,40(7):1623-1628.
    [8] 张慧,王涛,陈杰,等. 白芍多波长叠加指纹图谱研究[J]. 中药材,2017,40(9):2121-2124.
    [9] 李成义,马艳茹,李越峰,等. 西北地区商品甘草HPLC指纹图谱[J]. 中国实验方剂学杂志,2012, 18(9):129-132.
    [10] 钟永翠,别甜甜,翟文泽,等. 牡丹皮炮制前后HPLC指纹图谱比较[J]. 中国实验方剂学杂志,2016, 22(10):71-75.
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Establishment of HPLC fingerprint for Wenjing Tang

doi: 10.3969/j.issn.1006-0111.2018.06.006
  • 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China
  • 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;Shandong University of Chinese Medicine, Jinan 250355, China
  • Received Date: 2017-12-12
  • Rev Recd Date: 2018-06-21

Abstract: Objective To establish chromatographic fingerprint for Wenjing Tang by HPLC-DAD. Methods The fingerprint was determined by Waters Xselect HSS T3 column (4.6 mm×250 mm,5μm) with acetoniteile-0.1% formic acid solution as mobile phase for gradient elution. The temperature of column was 25℃ at a flow rate of 0.8 ml·min-1. The detective wavelength was set at 254 nm and ammonium glycyrrhizinate was selected as the reference peak. The HPLC fingerprints of 10 batches of Wenjing Tang were analyzed and the similarity was evaluated with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine. Results There were 39 common peaks in the HPLC fingerprints of Wenjing Tang and 6 common peaks were determined by the relative time of the standard substance. The similarities of 10 batches were all above 0.98 and the deviations among these batches are very small. Conclusion The HPLC-DAD fingerprint method for Wenjing Tang is accurate, stable and reliable. The fingerprint is the characteristic of the overall chemical components in Wenjing Tang. It can be used as an effective method for the quality control of Wenjing Tang.

CHAI Ruiping, LU Juan, ZHAO Ying, WANG Xiaojing, LÜ Xinkai, CHEN Xi. Establishment of HPLC fingerprint for Wenjing Tang[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006
Citation: CHAI Ruiping, LU Juan, ZHAO Ying, WANG Xiaojing, LÜ Xinkai, CHEN Xi. Establishment of HPLC fingerprint for Wenjing Tang[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(6): 503-506. doi: 10.3969/j.issn.1006-0111.2018.06.006
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