HUANG Jin, LIU Jingxue, ZENG Ying. Study of the effect of polysaccharides from Yupingfeng on keratinocytes[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(1): 51-54. doi: 10.3969/j.issn.1006-0111.2019.01.012
| Citation:
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HUANG Jin, LIU Jingxue, ZENG Ying. Study of the effect of polysaccharides from Yupingfeng on keratinocytes[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(1): 51-54. doi: 10.3969/j.issn.1006-0111.2019.01.012
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Study of the effect of polysaccharides from Yupingfeng on keratinocytes
- 1.
Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Pharmacy, Pudong New Area People's Hospital, Shanghai 201299, China
- 2.
Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
- 3.
Department of Pharmacy, Pudong New Area People's Hospital, Shanghai 201299, China
- Received Date: 2018-06-26
- Rev Recd Date:
2018-11-29
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Abstract
Objective To study the immunomodulatory effect of Yupingfeng polysaccharides on keratinocytes in vitro. Methods The effects of Yupingfeng polysaccharides on the expression of various inflammatory factors in the activated keratinocytes were detected by the Real-time PCR method.The mRNA and protein expression levels of Filaggrin were detected by Real-time PCR and Elisa respectively.The western bolt assay was used to detect the regulation of NF-κB signaling pathway by polysaccharides. Results Yupingfeng polysaccharides inhibited the secretion of inflammatory factors in the activated keratinocytes, promoted the expression of Filaggrin and exhibited significant inhibitory effect on the NF-κB signaling pathway, which plays an important role in the inflammation and immune response. Conclusion Yupingfeng polysaccharides had an immune regulation effect on skin keratinocytes in vitro and alleviated the development of local inflammation.These results lay a theoretical foundation for the clinical application of Yupingfeng prescription.
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