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WANG Xuelian, ZHENG Sili, LI Zhiyong, LUO Hengyu, MIAO Chaoyu. Construction and validation of a mouse model with systemic overexpression of human METRNL gene[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(5): 198-202, 222. doi: 10.12206/j.issn.2097-2024.202311014
Citation:
YANG Fanping, LI Guowen, XI Yan. Study on improvement of quality standard for Kechuan Liuwei oral liquid[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(1): 55-58,85. doi: 10.3969/j.issn.1006-0111.2019.01.013
Study on improvement of quality standard for Kechuan Liuwei oral liquid
Office of Clinical Trial Institution, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
2.
Department of Pharmacy, Shanghai Integrative Medical Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China
3.
Department of Pharmacy, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
Received Date: 2018-07-02
Rev Recd Date:
2018-09-10
Abstract
Objective To improve the quality control standard of the hospital preparation Kechuan Liuwei oral liquid in Longhua Hospital. Methods TLC was used for qualitative identification of Scutellaria baicalensis Georgi and Asarum sieboldi Mig in the Kechuan Liuwei oral liquid. Ephedrine and Baicalin content in ephedra and Scutellaria were determined by HPLC with Welch-C18 column (4.6mm×250mm, 5 μm). Acetonitrile-0.1% phosphoric acid solution (4:96) and methanol-0.1% phosphoric acid solution (47:53) were used as mobile phase. The detection wavelengths were 206 nm and 278 nm respectively. The flow rate was 1.0 ml/min. Results The TLC spots of Scutellaria baicalensis Georgi and asarum were clear without interference of the negative control. The linear range of Ephedrine hydrochloride was within 12.04-301.00 μg/ml (r=0.999 9). The average recovery was 101.7% (RSD=1.5%). The linear range of Pseudoephedrine hydrochloride was within 7.98-199.40 μg/ml (r=0.999 9). The average recovery was 101.6% (RSD=2.4%). The linear range of Baicalin was within 5.18-129.50 μg/ml (r=0.999 9). The average recovery was 101.0% (RSD=0.3%). Conclusion The qualitative identification and the active ingredient assay method established in this experiment were simple and feasible. Those methods can be used as the quality control standard for Kechuan Liuwei oral liquid.
1. Office of Clinical Trial Institution, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
2. Department of Pharmacy, Shanghai Integrative Medical Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China
3. Department of Pharmacy, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
Abstract: Objective To improve the quality control standard of the hospital preparation Kechuan Liuwei oral liquid in Longhua Hospital. Methods TLC was used for qualitative identification of Scutellaria baicalensis Georgi and Asarum sieboldi Mig in the Kechuan Liuwei oral liquid. Ephedrine and Baicalin content in ephedra and Scutellaria were determined by HPLC with Welch-C18 column (4.6mm×250mm, 5 μm). Acetonitrile-0.1% phosphoric acid solution (4:96) and methanol-0.1% phosphoric acid solution (47:53) were used as mobile phase. The detection wavelengths were 206 nm and 278 nm respectively. The flow rate was 1.0 ml/min. Results The TLC spots of Scutellaria baicalensis Georgi and asarum were clear without interference of the negative control. The linear range of Ephedrine hydrochloride was within 12.04-301.00 μg/ml (r=0.999 9). The average recovery was 101.7% (RSD=1.5%). The linear range of Pseudoephedrine hydrochloride was within 7.98-199.40 μg/ml (r=0.999 9). The average recovery was 101.6% (RSD=2.4%). The linear range of Baicalin was within 5.18-129.50 μg/ml (r=0.999 9). The average recovery was 101.0% (RSD=0.3%). Conclusion The qualitative identification and the active ingredient assay method established in this experiment were simple and feasible. Those methods can be used as the quality control standard for Kechuan Liuwei oral liquid.
WANG Xuelian, ZHENG Sili, LI Zhiyong, LUO Hengyu, MIAO Chaoyu. Construction and validation of a mouse model with systemic overexpression of human METRNL gene[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(5): 198-202, 222. doi: 10.12206/j.issn.2097-2024.202311014
Citation:
YANG Fanping, LI Guowen, XI Yan. Study on improvement of quality standard for Kechuan Liuwei oral liquid[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(1): 55-58,85. doi: 10.3969/j.issn.1006-0111.2019.01.013
WANG Xuelian, ZHENG Sili, LI Zhiyong, LUO Hengyu, MIAO Chaoyu. Construction and validation of a mouse model with systemic overexpression of human METRNL gene[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(5): 198-202, 222. doi: 10.12206/j.issn.2097-2024.202311014
WANG Xuelian, ZHENG Sili, LI Zhiyong, LUO Hengyu, MIAO Chaoyu. Construction and validation of a mouse model with systemic overexpression of human METRNL gene[J]. Journal of Pharmaceutical Practice and Service, 2024, 42(5): 198-202, 222. doi: 10.12206/j.issn.2097-2024.202311014
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