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SUN Huijuan, ZHAO Tao. Protective effects of Gallic acid on ionizing irradiated HIEC cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 299-303. doi: 10.3969/j.issn.1006-0111.2019.04.003
Citation:
SUN Huijuan, ZHAO Tao. Protective effects of Gallic acid on ionizing irradiated HIEC cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 299-303. doi: 10.3969/j.issn.1006-0111.2019.04.003
Protective effects of Gallic acid on ionizing irradiated HIEC cells
Department of Nursing, Weinan Vocational & Technical College, Weinan 714000, China;Department of Radiation Protective Medicine, Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Shaanxi Provincial Key Laboratory of Free Radical Biology and Medicine, Faculty of Military Preventive Medicine, Air Force Medical University, Xi'an 710032, China
2.
Department of Radiation Protective Medicine, Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Shaanxi Provincial Key Laboratory of Free Radical Biology and Medicine, Faculty of Military Preventive Medicine, Air Force Medical University, Xi'an 710032, China
Received Date: 2018-11-26
Rev Recd Date:
2019-04-23
Abstract
Objective To investigate the protective effect and primary mechanism of Gallic acid on 60Co-γ ray induced Human Intestinal Epithelial cell (HIEC) damage. Methods HIEC in the exponential phase were inoculated in petri dishes and were randomly divided into sham exposure group and exposure group.The exposure group was exposed to 4Gy 60Co-γ ray.CCK-8 method was used to detect cell proliferation ability.Clone formation method was used to observe the effects on clone formation ability,ELISA assay was used to evaluate the resistance effects on the lipid oxidation reaction,and Western-Blot test was used to observe the effects of GA on cycle protein Cyclin D1 and CDK4 in irradiated HIEC cells. Results 1.GA can promote the growth of HIEC cells 12h after GA treatment (P<0.05).But GA has a toxic effect on the HIEC growth 24h and 48h after the treatment.2.Compared with 4Gy 60Co-γ ray irradiation exposure group,GA(1,10,20 μmol/L) can increase the proliferation rate of HIEC cell significantly (P< 0.05).3.Compared with 4Gy 60Co-γ ray irradiation exposure group,pretreatment with GA(1,20 μmol/L) increased the clone formation rates of HIEC cell significantly (P<0.05).4.Compared with 4Gy 60Co-γ ray irradiation exposure group,SOD activity of GA(1,10,20μmol/L)treatment group increased significantly (P<0.05).GSH activity of 10 μmol/L GA treatment group increased significantly (P<0.05).5.The expression level of cell cycle protein Cyclin D1 and CDK4 in GA (1 μmol/L) treatment group were significantly increased compare with γ ray irradiation exposure group. Conclusion GA has protective effect on 60Co-γ ray irradiated HIEC cells.The mechanism may be related to the GA's capability as antioxidant,scavenging free radicals,regulating cell cycle and inhibiting cell apoptosis.
KARIMI-KHOUZANI O,HEIDARIAN E,AMINI S A.Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats[J].Pharmacol Rep,2017,69(4):830-835.
[7]
PERKINS C L,FANG G,KIM C N,et al.The role of Apaf-1,caspase-9,and bid proteins in etoposide or paclitaxel-induced mitochondrial events during apoptosis[J].Cancer Res,2000,60(6):1645-1653.
1. Department of Nursing, Weinan Vocational & Technical College, Weinan 714000, China;Department of Radiation Protective Medicine, Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Shaanxi Provincial Key Laboratory of Free Radical Biology and Medicine, Faculty of Military Preventive Medicine, Air Force Medical University, Xi'an 710032, China
2. Department of Radiation Protective Medicine, Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Shaanxi Provincial Key Laboratory of Free Radical Biology and Medicine, Faculty of Military Preventive Medicine, Air Force Medical University, Xi'an 710032, China
Abstract: Objective To investigate the protective effect and primary mechanism of Gallic acid on 60Co-γ ray induced Human Intestinal Epithelial cell (HIEC) damage. Methods HIEC in the exponential phase were inoculated in petri dishes and were randomly divided into sham exposure group and exposure group.The exposure group was exposed to 4Gy 60Co-γ ray.CCK-8 method was used to detect cell proliferation ability.Clone formation method was used to observe the effects on clone formation ability,ELISA assay was used to evaluate the resistance effects on the lipid oxidation reaction,and Western-Blot test was used to observe the effects of GA on cycle protein Cyclin D1 and CDK4 in irradiated HIEC cells. Results 1.GA can promote the growth of HIEC cells 12h after GA treatment (P<0.05).But GA has a toxic effect on the HIEC growth 24h and 48h after the treatment.2.Compared with 4Gy 60Co-γ ray irradiation exposure group,GA(1,10,20 μmol/L) can increase the proliferation rate of HIEC cell significantly (P< 0.05).3.Compared with 4Gy 60Co-γ ray irradiation exposure group,pretreatment with GA(1,20 μmol/L) increased the clone formation rates of HIEC cell significantly (P<0.05).4.Compared with 4Gy 60Co-γ ray irradiation exposure group,SOD activity of GA(1,10,20μmol/L)treatment group increased significantly (P<0.05).GSH activity of 10 μmol/L GA treatment group increased significantly (P<0.05).5.The expression level of cell cycle protein Cyclin D1 and CDK4 in GA (1 μmol/L) treatment group were significantly increased compare with γ ray irradiation exposure group. Conclusion GA has protective effect on 60Co-γ ray irradiated HIEC cells.The mechanism may be related to the GA's capability as antioxidant,scavenging free radicals,regulating cell cycle and inhibiting cell apoptosis.
SUN Huijuan, ZHAO Tao. Protective effects of Gallic acid on ionizing irradiated HIEC cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 299-303. doi: 10.3969/j.issn.1006-0111.2019.04.003
Citation:
SUN Huijuan, ZHAO Tao. Protective effects of Gallic acid on ionizing irradiated HIEC cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 299-303. doi: 10.3969/j.issn.1006-0111.2019.04.003
KARIMI-KHOUZANI O,HEIDARIAN E,AMINI S A.Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats[J].Pharmacol Rep,2017,69(4):830-835.
[7]
PERKINS C L,FANG G,KIM C N,et al.The role of Apaf-1,caspase-9,and bid proteins in etoposide or paclitaxel-induced mitochondrial events during apoptosis[J].Cancer Res,2000,60(6):1645-1653.
SUN Huijuan, ZHAO Tao. Protective effects of Gallic acid on ionizing irradiated HIEC cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 299-303. doi: 10.3969/j.issn.1006-0111.2019.04.003
SUN Huijuan, ZHAO Tao. Protective effects of Gallic acid on ionizing irradiated HIEC cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 299-303. doi: 10.3969/j.issn.1006-0111.2019.04.003
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