Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code

ZHANG Enhui, WANG Haifeng, CHEN Li, LIU Dianhua, YU Jianguang, CAI Guojun. Circadian rhythms of myocardial ischemia and chronotherapy[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 8-11,47. doi: 10.3969/j.issn.1006-0111.2016.01.003
Citation: SUN Huijuan, ZHAO Tao. Protective effects of Gallic acid on ionizing irradiated HIEC cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 299-303. doi: 10.3969/j.issn.1006-0111.2019.04.003

Protective effects of Gallic acid on ionizing irradiated HIEC cells

doi: 10.3969/j.issn.1006-0111.2019.04.003
  • Received Date: 2018-11-26
  • Rev Recd Date: 2019-04-23
  • Objective To investigate the protective effect and primary mechanism of Gallic acid on 60Co-γ ray induced Human Intestinal Epithelial cell (HIEC) damage. Methods HIEC in the exponential phase were inoculated in petri dishes and were randomly divided into sham exposure group and exposure group.The exposure group was exposed to 4Gy 60Co-γ ray.CCK-8 method was used to detect cell proliferation ability.Clone formation method was used to observe the effects on clone formation ability,ELISA assay was used to evaluate the resistance effects on the lipid oxidation reaction,and Western-Blot test was used to observe the effects of GA on cycle protein Cyclin D1 and CDK4 in irradiated HIEC cells. Results 1.GA can promote the growth of HIEC cells 12h after GA treatment (P<0.05).But GA has a toxic effect on the HIEC growth 24h and 48h after the treatment.2.Compared with 4Gy 60Co-γ ray irradiation exposure group,GA(1,10,20 μmol/L) can increase the proliferation rate of HIEC cell significantly (P< 0.05).3.Compared with 4Gy 60Co-γ ray irradiation exposure group,pretreatment with GA(1,20 μmol/L) increased the clone formation rates of HIEC cell significantly (P<0.05).4.Compared with 4Gy 60Co-γ ray irradiation exposure group,SOD activity of GA(1,10,20μmol/L)treatment group increased significantly (P<0.05).GSH activity of 10 μmol/L GA treatment group increased significantly (P<0.05).5.The expression level of cell cycle protein Cyclin D1 and CDK4 in GA (1 μmol/L) treatment group were significantly increased compare with γ ray irradiation exposure group. Conclusion GA has protective effect on 60Co-γ ray irradiated HIEC cells.The mechanism may be related to the GA's capability as antioxidant,scavenging free radicals,regulating cell cycle and inhibiting cell apoptosis.
  • [1] 郭娟,张琰君,安广洲,等.丹参素对于电离辐射损伤小鼠的防护作用[J].时珍国医国药,2015,26(8):1811-1813.
    [2] 郑雪花,杨君,杨跃辉.没食子酸药理作用的研究进展[J].中国医院药学杂志,2017,37(1):94-98.
    [3] 孙会娟,张瑜,赵涛.中药五倍子及其活性成分的研究进展[J].基层医学论坛,2018,22(19):2728-2730.
    [4] 陈胜,刘珂,秦珊,等.CCK-8法检测蓝光和白光对ARPE-19细胞增殖的影响[J].国际眼科杂志,2018,18(08):1385-1388.
    [5] 胡雅梦,刘琅嬛,刘宝婵,等.黄芪甲苷对肝细胞的辐射防护作用[J].辐射研究与辐射工艺学报,2015,12(33):1021-1026.
    [6] KARIMI-KHOUZANI O,HEIDARIAN E,AMINI S A.Anti-inflammatory and ameliorative effects of gallic acid on fluoxetine-induced oxidative stress and liver damage in rats[J].Pharmacol Rep,2017,69(4):830-835.
    [7] PERKINS C L,FANG G,KIM C N,et al.The role of Apaf-1,caspase-9,and bid proteins in etoposide or paclitaxel-induced mitochondrial events during apoptosis[J].Cancer Res,2000,60(6):1645-1653.
    [8] 杨阳,陶仕英,牛建昭,等.紫草素对宫颈癌SiHa细胞增殖周期的影响[J].环球中医药,2018,11(01):6-10.
  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-040123456Highcharts.com
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 86.3 %FULLTEXT: 86.3 %META: 9.9 %META: 9.9 %PDF: 3.8 %PDF: 3.8 %FULLTEXTMETAPDFHighcharts.com
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 21.5 %其他: 21.5 %其他: 0.2 %其他: 0.2 %China: 0.8 %China: 0.8 %Falls Church: 0.2 %Falls Church: 0.2 %[]: 0.8 %[]: 0.8 %三亚: 0.2 %三亚: 0.2 %三明: 0.2 %三明: 0.2 %上海: 1.6 %上海: 1.6 %中卫: 0.2 %中卫: 0.2 %乐山: 0.2 %乐山: 0.2 %佛山: 0.2 %佛山: 0.2 %北京: 19.3 %北京: 19.3 %十堰: 0.8 %十堰: 0.8 %南京: 0.4 %南京: 0.4 %南平: 0.2 %南平: 0.2 %厦门: 0.2 %厦门: 0.2 %台州: 0.6 %台州: 0.6 %周口: 0.2 %周口: 0.2 %嘉峪关: 0.2 %嘉峪关: 0.2 %天水: 0.2 %天水: 0.2 %威海: 0.2 %威海: 0.2 %宿迁: 0.6 %宿迁: 0.6 %岳阳: 0.4 %岳阳: 0.4 %广州: 0.2 %广州: 0.2 %张家口: 1.2 %张家口: 1.2 %惠州: 0.2 %惠州: 0.2 %成都: 0.4 %成都: 0.4 %新乡: 0.2 %新乡: 0.2 %无锡: 0.2 %无锡: 0.2 %昌吉: 0.2 %昌吉: 0.2 %杭州: 1.0 %杭州: 1.0 %格兰特县: 0.8 %格兰特县: 0.8 %梅州: 0.2 %梅州: 0.2 %榆林: 0.2 %榆林: 0.2 %武汉: 0.8 %武汉: 0.8 %泰安: 0.2 %泰安: 0.2 %泸州: 0.2 %泸州: 0.2 %济南: 0.2 %济南: 0.2 %海口: 0.2 %海口: 0.2 %淄博: 0.2 %淄博: 0.2 %渥太华: 0.4 %渥太华: 0.4 %滁州: 0.2 %滁州: 0.2 %漳州: 0.2 %漳州: 0.2 %濮阳: 0.2 %濮阳: 0.2 %盐城: 0.2 %盐城: 0.2 %石家庄: 0.2 %石家庄: 0.2 %秦皇岛: 0.4 %秦皇岛: 0.4 %绵阳: 0.2 %绵阳: 0.2 %美国伊利诺斯芝加哥: 1.2 %美国伊利诺斯芝加哥: 1.2 %聊城: 0.2 %聊城: 0.2 %芒廷维尤: 19.7 %芒廷维尤: 19.7 %蚌埠: 0.4 %蚌埠: 0.4 %衡水: 0.2 %衡水: 0.2 %衡阳: 0.2 %衡阳: 0.2 %西宁: 15.5 %西宁: 15.5 %西安: 1.0 %西安: 1.0 %运城: 0.4 %运城: 0.4 %郑州: 0.2 %郑州: 0.2 %鄂州: 0.2 %鄂州: 0.2 %重庆: 0.6 %重庆: 0.6 %锦州: 0.2 %锦州: 0.2 %长春: 0.2 %长春: 0.2 %长沙: 0.2 %长沙: 0.2 %长治: 0.4 %长治: 0.4 %青岛: 0.2 %青岛: 0.2 %黄冈: 0.8 %黄冈: 0.8 %其他其他ChinaFalls Church[]三亚三明上海中卫乐山佛山北京十堰南京南平厦门台州周口嘉峪关天水威海宿迁岳阳广州张家口惠州成都新乡无锡昌吉杭州格兰特县梅州榆林武汉泰安泸州济南海口淄博渥太华滁州漳州濮阳盐城石家庄秦皇岛绵阳美国伊利诺斯芝加哥聊城芒廷维尤蚌埠衡水衡阳西宁西安运城郑州鄂州重庆锦州长春长沙长治青岛黄冈Highcharts.com
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(3702) PDF downloads(330) Cited by()

Related
Proportional views

Protective effects of Gallic acid on ionizing irradiated HIEC cells

doi: 10.3969/j.issn.1006-0111.2019.04.003

Abstract: Objective To investigate the protective effect and primary mechanism of Gallic acid on 60Co-γ ray induced Human Intestinal Epithelial cell (HIEC) damage. Methods HIEC in the exponential phase were inoculated in petri dishes and were randomly divided into sham exposure group and exposure group.The exposure group was exposed to 4Gy 60Co-γ ray.CCK-8 method was used to detect cell proliferation ability.Clone formation method was used to observe the effects on clone formation ability,ELISA assay was used to evaluate the resistance effects on the lipid oxidation reaction,and Western-Blot test was used to observe the effects of GA on cycle protein Cyclin D1 and CDK4 in irradiated HIEC cells. Results 1.GA can promote the growth of HIEC cells 12h after GA treatment (P<0.05).But GA has a toxic effect on the HIEC growth 24h and 48h after the treatment.2.Compared with 4Gy 60Co-γ ray irradiation exposure group,GA(1,10,20 μmol/L) can increase the proliferation rate of HIEC cell significantly (P< 0.05).3.Compared with 4Gy 60Co-γ ray irradiation exposure group,pretreatment with GA(1,20 μmol/L) increased the clone formation rates of HIEC cell significantly (P<0.05).4.Compared with 4Gy 60Co-γ ray irradiation exposure group,SOD activity of GA(1,10,20μmol/L)treatment group increased significantly (P<0.05).GSH activity of 10 μmol/L GA treatment group increased significantly (P<0.05).5.The expression level of cell cycle protein Cyclin D1 and CDK4 in GA (1 μmol/L) treatment group were significantly increased compare with γ ray irradiation exposure group. Conclusion GA has protective effect on 60Co-γ ray irradiated HIEC cells.The mechanism may be related to the GA's capability as antioxidant,scavenging free radicals,regulating cell cycle and inhibiting cell apoptosis.

ZHANG Enhui, WANG Haifeng, CHEN Li, LIU Dianhua, YU Jianguang, CAI Guojun. Circadian rhythms of myocardial ischemia and chronotherapy[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 8-11,47. doi: 10.3969/j.issn.1006-0111.2016.01.003
Citation: SUN Huijuan, ZHAO Tao. Protective effects of Gallic acid on ionizing irradiated HIEC cells[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 299-303. doi: 10.3969/j.issn.1006-0111.2019.04.003
Reference (8)

Catalog

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return