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ZHOU Jing, YE Zhaohui, WU Yanhua. The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 337-341,347. doi: 10.3969/j.issn.1006-0111.2019.04.010
Citation: ZHOU Jing, YE Zhaohui, WU Yanhua. The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 337-341,347. doi: 10.3969/j.issn.1006-0111.2019.04.010

The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro

doi: 10.3969/j.issn.1006-0111.2019.04.010
  • Received Date: 2018-12-20
  • Rev Recd Date: 2019-05-31
  • Objective To establish a method for simultaneous determination of three triterpenoid saponins (BeesiosideⅠ、Ⅱ、Ⅲ),and investigate antioxidant effects of triterpenoid saponin extracted from Bessia calthaefolia. Methods The content assay of triterpenoid saponin extracted from Bessia calthaefolia was conducted by high performance liquid chromatography method with evaporative light-scattering detector (HPLC-ELSD).The methodology validation was performed to confirm the content determination.The free radicals scavenging activities and antioxidant effects of the triterpenoid saponin were evaluated in vitro. Results The HPLC-ELSD method can simultaneously determine the content of Beesioside I,Ⅱ and Ⅲ.It has good linearity,precision,repeatability and stability with high recovery.The triterpenoid saponins showed great activity on scavenging ABTS+、DPPH·and OH free radicals and antioxidant effects. Conclusion The HPLC-ELSD method has good specificity,stability,repeatability and accuracy.This method can be used for content determination and quality standard of three triterpenoid saponins (BeesiosideⅠ、Ⅱ、Ⅲ) from Bessia calthaefolia.The triterpenoid saponins exhibited free radicals scavenging effects and antioxidant effects.
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    [3] SAKURAI N,NAGAI M,NAGASE H,et al.Studies on the constituents of Beesia calthaefolia and Souliea vaginata.Ⅱ:Beesioside Ⅱ,a cyclolanostanol xyloside from rhizome of Beesia calthaefolia[J].Chem Pharm Bull,1986,34(2):582-589.
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The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro

doi: 10.3969/j.issn.1006-0111.2019.04.010

Abstract: Objective To establish a method for simultaneous determination of three triterpenoid saponins (BeesiosideⅠ、Ⅱ、Ⅲ),and investigate antioxidant effects of triterpenoid saponin extracted from Bessia calthaefolia. Methods The content assay of triterpenoid saponin extracted from Bessia calthaefolia was conducted by high performance liquid chromatography method with evaporative light-scattering detector (HPLC-ELSD).The methodology validation was performed to confirm the content determination.The free radicals scavenging activities and antioxidant effects of the triterpenoid saponin were evaluated in vitro. Results The HPLC-ELSD method can simultaneously determine the content of Beesioside I,Ⅱ and Ⅲ.It has good linearity,precision,repeatability and stability with high recovery.The triterpenoid saponins showed great activity on scavenging ABTS+、DPPH·and OH free radicals and antioxidant effects. Conclusion The HPLC-ELSD method has good specificity,stability,repeatability and accuracy.This method can be used for content determination and quality standard of three triterpenoid saponins (BeesiosideⅠ、Ⅱ、Ⅲ) from Bessia calthaefolia.The triterpenoid saponins exhibited free radicals scavenging effects and antioxidant effects.

ZHOU Jing, YE Zhaohui, WU Yanhua. The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 337-341,347. doi: 10.3969/j.issn.1006-0111.2019.04.010
Citation: ZHOU Jing, YE Zhaohui, WU Yanhua. The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 337-341,347. doi: 10.3969/j.issn.1006-0111.2019.04.010
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