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ZHANG Juanjuan, YU Hai, WU Zhihong, XU Shun, ZHANG Lichao. The effect of triamcinolone acetonide delivered by microneedle roller on nude mouse model xenografted with human hypertrophic scar[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 342-347. doi: 10.3969/j.issn.1006-0111.2019.04.011
Citation: ZHANG Juanjuan, YU Hai, WU Zhihong, XU Shun, ZHANG Lichao. The effect of triamcinolone acetonide delivered by microneedle roller on nude mouse model xenografted with human hypertrophic scar[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 342-347. doi: 10.3969/j.issn.1006-0111.2019.04.011

The effect of triamcinolone acetonide delivered by microneedle roller on nude mouse model xenografted with human hypertrophic scar

doi: 10.3969/j.issn.1006-0111.2019.04.011
  • Received Date: 2019-02-24
  • Rev Recd Date: 2019-06-25
  • Objective To investigate the effect of triamcinolone acetonide cream (TAC) delivered by microneedle roller on the treatment for human hypertrophic scar. Methods The model was established by xenografting human hypertrophic scar onto the back of nude mouse.Seventy-two nude mice were randomly divided into TAC itself,TAC with microneedle and intralesional injection group with 24 mice in each group.Six nude mice from each group were euthanized at 15,30,45 and 60 days after treatment.Xenografted scars were harvested for histologic analysis.The expression of CD34 and α-smooth muscle actin(α-SMA) were detected by immunohistochemistry.Cell apoptosis was detected by TUNEL method. Results 60 days after the treatment,there was no significant morphology change in TAC group.The scar was shrunk,soft and flat with lighter color in the microneedle and injection groups.The values of CD34 expression,α-smooth muscle actin and cell apoptosis in TAC group were 15.83±1.84,47.36±1.95,and 21.50±3.62,respectively,which had no significant difference compared with those after 15 days treatment(P>0.05).The decrease of the expression of CD34,α-SMA(P<0.05)and the increase of apoptotic cells(P<0.05)were observed 30 days after the microneedle treatment and 15 days after intralesional injection.60 days after the administration,the value of CD34 expression,α-SMA,and cell apoptosis in those two groups were 7.83±0.75,9.87±1.96,26.30±8.48 and 27.23±4.68,34.00±1.55,38.67±3.98,respectively,which had significant difference compared to TAC group(P<0.05).There was no significant difference (P>0.05) between the microneedle group and the injection group in the expression of CD34,α-smooth muscle actin,and cell apoptosis. Conclusion The anti-scar effect of triamcinolone acetonide delivered by microneedle roller was similar to that by intralesional injection in the nude mouse model.
  • [1] GAUGLITZ GG.Management of keloids and hypertrophic scars:current and emerging options[J].Clin Cosmet Investig Dermatol,2013,6:103-114.
    [2] 李雅,张娟娟,张立超,等.改装滚轮微针促进人增生性瘢痕皮肤对醋酸曲安奈德的吸收[J].第二军医大学学报,2015,36(1):58-64.
    [3] 张娟娟,张立超,朱全刚,等.改装滚轮微针用于醋酸曲安奈德经皮给药的特性试验[J].中国医院药学杂志,2015,35(4):292-296.
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    [5] DAROUGHEH A,ASILIAN A,SHARIATI F.Intralesional triamcinolone alone or in combination with 5-fluorouracil for the treatment of keloid and hypertrophic scars[J].Clin Exp Dermatol,2009,34(2):219-223.
    [6] REISH R G,ERIKSSON E.Scar treatments:preclinical and clinical studies[J].J Am Coll Surg,2008,206(4):719-730.
    [7] MARTANTO W,DAVIS S P,HOLIDAY N R,et al.Transdermal delivery of insulin using microneedlesin vivo[J].Pharm Res,2004,21(6):947-952.
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The effect of triamcinolone acetonide delivered by microneedle roller on nude mouse model xenografted with human hypertrophic scar

doi: 10.3969/j.issn.1006-0111.2019.04.011

Abstract: Objective To investigate the effect of triamcinolone acetonide cream (TAC) delivered by microneedle roller on the treatment for human hypertrophic scar. Methods The model was established by xenografting human hypertrophic scar onto the back of nude mouse.Seventy-two nude mice were randomly divided into TAC itself,TAC with microneedle and intralesional injection group with 24 mice in each group.Six nude mice from each group were euthanized at 15,30,45 and 60 days after treatment.Xenografted scars were harvested for histologic analysis.The expression of CD34 and α-smooth muscle actin(α-SMA) were detected by immunohistochemistry.Cell apoptosis was detected by TUNEL method. Results 60 days after the treatment,there was no significant morphology change in TAC group.The scar was shrunk,soft and flat with lighter color in the microneedle and injection groups.The values of CD34 expression,α-smooth muscle actin and cell apoptosis in TAC group were 15.83±1.84,47.36±1.95,and 21.50±3.62,respectively,which had no significant difference compared with those after 15 days treatment(P>0.05).The decrease of the expression of CD34,α-SMA(P<0.05)and the increase of apoptotic cells(P<0.05)were observed 30 days after the microneedle treatment and 15 days after intralesional injection.60 days after the administration,the value of CD34 expression,α-SMA,and cell apoptosis in those two groups were 7.83±0.75,9.87±1.96,26.30±8.48 and 27.23±4.68,34.00±1.55,38.67±3.98,respectively,which had significant difference compared to TAC group(P<0.05).There was no significant difference (P>0.05) between the microneedle group and the injection group in the expression of CD34,α-smooth muscle actin,and cell apoptosis. Conclusion The anti-scar effect of triamcinolone acetonide delivered by microneedle roller was similar to that by intralesional injection in the nude mouse model.

ZHANG Juanjuan, YU Hai, WU Zhihong, XU Shun, ZHANG Lichao. The effect of triamcinolone acetonide delivered by microneedle roller on nude mouse model xenografted with human hypertrophic scar[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 342-347. doi: 10.3969/j.issn.1006-0111.2019.04.011
Citation: ZHANG Juanjuan, YU Hai, WU Zhihong, XU Shun, ZHANG Lichao. The effect of triamcinolone acetonide delivered by microneedle roller on nude mouse model xenografted with human hypertrophic scar[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 342-347. doi: 10.3969/j.issn.1006-0111.2019.04.011
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