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Volume 37 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2019  >  37(4): 348-351

LI Qing, HE Mingyue, QU Falin, TAN Xingqi, DONG Wenshen, NI Yujia, SHAO Liuying. Determination of content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 348-351. doi: 10.3969/j.issn.1006-0111.2019.04.012
Citation: LI Qing, HE Mingyue, QU Falin, TAN Xingqi, DONG Wenshen, NI Yujia, SHAO Liuying. Determination of content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 348-351. doi: 10.3969/j.issn.1006-0111.2019.04.012
shu

Determination of content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC

doi: 10.3969/j.issn.1006-0111.2019.04.012

  • Hospital 904 of Joint Logistic Support Force of PLA, Changzhou 213003, China

  • Received Date: 2018-09-27
  • Rev Recd Date: 2019-03-07
  • Objective To determine the content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC. Methods The chromatographic column was Lichrospher C18 (150 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.05% H3PO4 solution (47:53) with a flow rate of 1.0 ml·min-1,the UV detection wavelength at 313 nm and the column temperature at 30℃. Results There was a good linear relationship for α-Asarone in the range of 0.097 0~1.934 μg (r=0.999 8).The average recovery was 99.94% (RSD=1.13%).The sample was stable within 24 h.The method had good repeatability. Conclusions This method is simple and accurate for the content assay of α-Asarone in Piper sarmentosum Roxb.It can be used to control the quality of Piper sarmentosum Roxb.There was a significant difference of α-Asarone content in Piper sarmentosum Roxb.from different provinces in China.The Piper sarmentosum Roxb.from Guangxi province has the highest content of α-Asarone.
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    [3] 广西壮族自治区食品药品监督管理局.广西壮族自治区壮药质量标准[S].南宁:广西科技出版社,2011:270.
    [4] AMRAN A A,ZAKARIA Z,OTHMAN F,et al.Aqueous extract of Piper sarmentosum decreases atherosclerotic lesions in high cholesterolemic experimental rabbits[J].Lipids Health Dis,2010,9(1):44.
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    [7] SAWANGJAROEN N,SAWANGJAROEN K,POONPANANG P.Effects of Piper longum fruit,Piper sarmentosum root and Quercus infectoria nut gall on caecal amoebiasis in mice[J].J Ethnopharmacol,2004,91(2-3):357-360.
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    [10] 蔡毅,董栋,么春艳,等.GC-MS分析广西产4种栽培胡椒属植物叶中的挥发油成分[J].华西药学杂志,2010,25(6):641-644.
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Determination of content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC

doi: 10.3969/j.issn.1006-0111.2019.04.012
  • Hospital 904 of Joint Logistic Support Force of PLA, Changzhou 213003, China
  • Received Date: 2018-09-27
  • Rev Recd Date: 2019-03-07

Abstract: Objective To determine the content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC. Methods The chromatographic column was Lichrospher C18 (150 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.05% H3PO4 solution (47:53) with a flow rate of 1.0 ml·min-1,the UV detection wavelength at 313 nm and the column temperature at 30℃. Results There was a good linear relationship for α-Asarone in the range of 0.097 0~1.934 μg (r=0.999 8).The average recovery was 99.94% (RSD=1.13%).The sample was stable within 24 h.The method had good repeatability. Conclusions This method is simple and accurate for the content assay of α-Asarone in Piper sarmentosum Roxb.It can be used to control the quality of Piper sarmentosum Roxb.There was a significant difference of α-Asarone content in Piper sarmentosum Roxb.from different provinces in China.The Piper sarmentosum Roxb.from Guangxi province has the highest content of α-Asarone.

LI Qing, HE Mingyue, QU Falin, TAN Xingqi, DONG Wenshen, NI Yujia, SHAO Liuying. Determination of content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 348-351. doi: 10.3969/j.issn.1006-0111.2019.04.012
Citation: LI Qing, HE Mingyue, QU Falin, TAN Xingqi, DONG Wenshen, NI Yujia, SHAO Liuying. Determination of content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 348-351. doi: 10.3969/j.issn.1006-0111.2019.04.012
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