The determination of thyotropin-releasing hormone in plasma by ion pair Rp-HPLC

Liu Zhengliang; Li Mingxiu; Cui Zhipin

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Volume 13 Issue 4

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Article Navigation > Journal of Pharmaceutical Practice and Service > 1995 > (4): 234-236

Liu Zhengliang, Li Mingxiu, Cui Zhipin. The determination of thyotropin-releasing hormone in plasma by ion pair Rp-HPLC[J]. Journal of Pharmaceutical Practice and Service, 1995, (4): 234-236.

Citation:

Liu Zhengliang, Li Mingxiu, Cui Zhipin. The determination of thyotropin-releasing hormone in plasma by ion pair Rp-HPLC[J]. Journal of Pharmaceutical Practice and Service, 1995, (4): 234-236.

The determination of thyotropin-releasing hormone in plasma by ion pair Rp-HPLC

1.
College of Pharmacy, Second Military Medical University, Shanghai 200433
2.
College of Pharmacy, Second Military Medical University, Shanghai 200433

Abstract

The method for the determination of Thyrotropin-Releasing Hormore in plasma by ion pair RP-HPLC was studied.(column:ODS:mobile phase:0.02mol/1.HAC-acetonitril-sodium 1-heptanesulfonate (89.85:10:0.15):flow rate:1.5ml/min detector:UV 220nm,0.1UFS.The average recovery was 101.69 ± 1.31%. The relation coefficient reached 0.9998.The method was simple and gave correct result and better reproducibility.
- ION pair,
- RP-HPLC,
- thyrotropin-releasing hormone,
- plasma concentration
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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