Determination of sodium, kalium, magesium and calcium in liquor of cardioplegia by high performance liquor chromatography-indirect photothetric determination method
Abstract: To determine the content of sodium,kalium, magnesium and calcium in liquor of cardioplegia by HPLC-IPD method.METHOD: The chromatographic system consisted of Soherisorb SCX column and mobile phase of the solution of 0.35 mmol/L cerium sulphate in water-acetonitrile(85:15), that were regulated to pH 3.2~3.4 with phosphoric acid.Ceritnn was a balance-ion in mobile phase, therefore the content of sodium, kalium, magnesium and calcium were determined directly by photometer.The detected wave length was UV254 um.RESULTS:The mean recovery of 4 ion were between in 97~104% and deteCtion limits of sodium,kalium,magnesium and calcium were 5, 8, 5 and 4 ng.There was noremarkable betWeen in this HPLC-IPD method and titrative method.CONCLUSION: The method is constant, sensitive, have a concentration and is good for determination of sodium, kalium, magnesium and calcium concentration.
Citation: | Yuan Cheng, Wang Jingxiang, Liang Zhu, . Determination of sodium, kalium, magesium and calcium in liquor of cardioplegia by high performance liquor chromatography-indirect photothetric determination method[J]. Journal of Pharmaceutical Practice and Service, 1997, (4): 234-237. |