Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 16 Issue 5
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 1998  >  (5): 296-298

Chen Jian, Fang Weijun, Zhang Dengling, Ding Zongzheng, Chen Rui. Determination of paracetamol in human serum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 1998, (5): 296-298.
Citation: Chen Jian, Fang Weijun, Zhang Dengling, Ding Zongzheng, Chen Rui. Determination of paracetamol in human serum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 1998, (5): 296-298.
shu

Determination of paracetamol in human serum by HPLC

  • Shanghai Songiiang Center Hospital, Shanghai 201600

  • An assay for paracetamol in human serum was developed using reverse phase high-performance liquid chromatographic with UV detection at 244nm. Analytial column of YWG C18 is used. The mobile phase consisted of a mixture of methanol: acetic acid-sodium acetate buffer solution: diethylamine (20:80:0.5)at the flow rate of 1.5ml/min. Theophylline is used as internal standard. Methanol is used for the sedimentation of protein. And minimum detective concentaion 0.05μg/ml. The standard curve is linear in the rang of 0.25~25μg/ml,the correlation coefficient is 0.9999.The method is suitable for the determination of human serum paracetamol and pharmacokinetic reserches.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2593) PDF downloads(351) Cited by()

Related
Proportional views

Determination of paracetamol in human serum by HPLC

  • Shanghai Songiiang Center Hospital, Shanghai 201600

Abstract: An assay for paracetamol in human serum was developed using reverse phase high-performance liquid chromatographic with UV detection at 244nm. Analytial column of YWG C18 is used. The mobile phase consisted of a mixture of methanol: acetic acid-sodium acetate buffer solution: diethylamine (20:80:0.5)at the flow rate of 1.5ml/min. Theophylline is used as internal standard. Methanol is used for the sedimentation of protein. And minimum detective concentaion 0.05μg/ml. The standard curve is linear in the rang of 0.25~25μg/ml,the correlation coefficient is 0.9999.The method is suitable for the determination of human serum paracetamol and pharmacokinetic reserches.

Chen Jian, Fang Weijun, Zhang Dengling, Ding Zongzheng, Chen Rui. Determination of paracetamol in human serum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 1998, (5): 296-298.
Citation: Chen Jian, Fang Weijun, Zhang Dengling, Ding Zongzheng, Chen Rui. Determination of paracetamol in human serum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 1998, (5): 296-298.
shu

    HTML

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return