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Volume 18 Issue 5
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2000  >  (5): 284-285

Isolation,Purification and characterization of NV-PP-4 from Nidus Vespae[J]. Journal of Pharmaceutical Practice and Service, 2000, (5): 284-285.
Citation: Isolation,Purification and characterization of NV-PP-4 from Nidus Vespae[J]. Journal of Pharmaceutical Practice and Service, 2000, (5): 284-285.
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Isolation,Purification and characterization of NV-PP-4 from Nidus Vespae

  • Received Date: 2000-08-28
  • OBJECTIVE To isolate and purify a new protein, nominated as NV-PP-4 from the anti-inflammatory of Nidus Vespae and study its characteristics.METHODS It was purified by Sephadex G-50 chromatography, followed by DEAE-Sephadex A50 anion exchange chromatography, hydroxyapatite column chromatography and high performance liquid chromatography, then it was characterized by HPLC, HPCE, amino acid analysis. RESULTS HPLC and IEF-HPCE showed that it was homogenous both in molecular weight and electric charge, its molecular weight was determined to be 8.711KD by HPLC. Its PI was estimated to be 1.79 by IEF-HPCE. Amino acid analysis showed that it consists of 87 amino acids and it was rich in Gly、Asp、Pro、Glu. CONCLUSION NV-Pp-4 was a new protein purified from the water extract of Nidus Vespae.
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Isolation,Purification and characterization of NV-PP-4 from Nidus Vespae

  • Received Date: 2000-08-28

Abstract: OBJECTIVE To isolate and purify a new protein, nominated as NV-PP-4 from the anti-inflammatory of Nidus Vespae and study its characteristics.METHODS It was purified by Sephadex G-50 chromatography, followed by DEAE-Sephadex A50 anion exchange chromatography, hydroxyapatite column chromatography and high performance liquid chromatography, then it was characterized by HPLC, HPCE, amino acid analysis. RESULTS HPLC and IEF-HPCE showed that it was homogenous both in molecular weight and electric charge, its molecular weight was determined to be 8.711KD by HPLC. Its PI was estimated to be 1.79 by IEF-HPCE. Amino acid analysis showed that it consists of 87 amino acids and it was rich in Gly、Asp、Pro、Glu. CONCLUSION NV-Pp-4 was a new protein purified from the water extract of Nidus Vespae.

Isolation,Purification and characterization of NV-PP-4 from Nidus Vespae[J]. Journal of Pharmaceutical Practice and Service, 2000, (5): 284-285.
Citation: Isolation,Purification and characterization of NV-PP-4 from Nidus Vespae[J]. Journal of Pharmaceutical Practice and Service, 2000, (5): 284-285.
shu

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