Determination of norvancomycin in cerebrospinal fluid by HPLC
- Received Date: 2000-07-20
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Key words:
- norvancomycin /
- cerebrospinal fluid /
- HPLC
Abstract: OBJECTIVE To establish a HPLC method for the determination of norvancomycin in cerebrospinal fluid.METHODS With tinidazole as the internal standard, the content of norvancomycin was assayed on a ODS-hypersil C18 column with a mobile phase composed of methanol-phosphate buffer solution(16:84,pH=5.0). The detection wavelength was 280nm.RESULTS Concentration compared with the peak area ratio, the linearity was obtained over the range of 1~60 μg.ml-1 (r=0.9993).The average recovery was 98.70%±2.65% and the intra-day and the inter-day RSD were within 1.73%~1.95% and 3.62~6.78% respectively.
Citation: | XU Jun, WANG-Qinmin. Determination of norvancomycin in cerebrospinal fluid by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2000, (6): 379-380. |