Linear determination of puerarin and isoflavones in Radix Puerariae in Plasma
- Received Date: 2002-02-15
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Key words:
- HPLC /
- puerarin /
- isoflavones
Abstract: OBJECTIVE To study the pharmacokinetics behavior of puerarin, isoflavones and its Products in human body, make the determination of puerarin and isoflavones in Radix Puerariae. METHODS Carry out the determination by HPLC. The chromatographic condition: the flow rate was 1mL·min-1. The temperature of the column was 20C:the detection wavelength was at 250nm. The mobile phase was consisted of methanol-water(25:75). RESULTS R = 6.56 RSD <5.02%. The average recoveries:(100.9 + 0.5)%, Linear range is 0.0295~0.708μg·mL-1 for puerarin and isoflavones in human plasma. CONCLUSIONS Pharmacokinetics of Puerarin and isoflavones in human plasma could be determined by HPLC, supplying the method for examining isoflavones in Radix Puerariae in human body.
Citation: | GUO Jian-ping, LI Guo-dong, ZHAO Tong-ying, FAN Wei. Linear determination of puerarin and isoflavones in Radix Puerariae in Plasma[J]. Journal of Pharmaceutical Practice and Service, 2003, (4): 219-221. |