Determination of zoledronic acid for injection by HPLC

Key words: 
• zoledronic acid for injection /
• determination /
• HPLC

Abstract: OBJECTIVE To establish an HPLC method for the determination of zoledronic acid for injection. METHOD:The analysis was achieved by using Diamonsil C₁₈ column with acetonitrile-ion-pair buffer solution(25:75)as mobile phase and UV-detector at wavelength 210nm. The ion-pair buffer solution was a mixture of 0.02mmo1/mL tetrabutyl ammonium hydroxide and 0.02mmo1/mL ammonium dihydrogen phosphate adjusted pH 2.3 with 50% phosphoric acid RESULTS:The linear range was from 50 to 240μg/mL(r=1.0). The average recovery was 100.0%(RSD=0.4%).CONCLUSIONS This method is simple,sensitive,accurate and suitable for the determination of zoledronic acid for injection.