Determination of dl-Tetrahydropamatine in Yanhusuo by HPLC
- Received Date: 2003-10-28
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Key words:
- Yanhusuo /
- dl-Tetrahydropamatine /
- HPLC
Abstract: OBJECTIVE An HPLC method was developed for determination of dl-Tetrahydropamatine in Yan-husuo. METHOD:The column was ODS Agilent zobax Extend C18(4.6×250mm,5μm),mobile phase was Meth-anol-0.1%. phosphoric acid(adjust pH 6.0 with Triethylamine)(55:45). Detection wavelength was set at 280nm. RESULT:Linearity range was 115-1376ng. The average recovery was 99.2%, RSD=1.5%(n=6). CONCLUSION The method is accurate and can be used for quality control of Yanhusuo.
Citation: | MA Lin-ke. Determination of dl-Tetrahydropamatine in Yanhusuo by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2003, (6): 351-353. |